Agar-gel precipitin reactions in experimental paragonimiasis

Original Article


Korean J Parasitol. 1981 Dec;19(2):101-108. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1981.19.2.101
Copyright © 1981 by The Korean Society for Parasitology


Agar-gel precipitin reactions in experimental paragonimiasis
Won-Young Choi and Ok-Ran Lee
Department of Parasitology, Catholic Medical College, Seoul, Korea.


Abstract
In an attempt to investigate the sensitivity of immunodiagnosis in cats experimentally infected with Paragonimus westermani, agar-gel precipitin reaction were studied. Metacercariae of P. westermani were administered to cats in various doses(2~100 metacercariae per cat) and antisera were obtained at an interval of a week. 1. Precipitin bands appeared in homologous antigen-antibody in experimental paragonimiasis between 3 and 5 weeks after infection in all the cats. 2. Almost all the cases in which a large number of worms were detected, showed strong reactions as revealed by deeply stained bands. 3. Precipitin reactions did not necessarily parallel with the number of worms detected. This may be attributable to the individual difference of a cat's conditions. 4. Very weak precipitin reactions were noticed between Clonorchis antigen and Paragonimus antisera of cats, but no reactions were noticed between Paragonimus antigen and Clonorchis antisera of cats or rabbits.

Figures

Fig. 1
Typical precipitin bands in each worm bur-den group of cat paragonimiasis,shown chronologically by week.

Center wells: Paragonimus antigen(=PA)

Outer wells: Weekly serum samples from experimental cats. C; control serum before infection. Numbers; serum of postinfection in weeks.

C1: Cat 100 metacercariae(=MC) given, C9: 50MC given, C13: 25MC given, C17: 10MC given, C25: 5MC given cat C29: 2MC given.


	Fig. 2 Strong of precipitin reactions at 18th week of infection when Paragonimus antigen (centre wells) and cat antiserum (outer wells) are diluted. S: undiluted serum.


	Fig. 3 Same as Fig. 2 (Cat No. 9).


	Fig. 4 Same as Fig. 2 (Cat No. 15).


	Fig. 5 Same as Fig. 2 (Cat No. 19).


	Fig. 6 Same as Fig. 2 (Cat No. 21).


	Fig. 7 Same as Fig. 2 (Cat No. 29).


	Fig. 8 Examples of very weak precipitin reaction in some experimental cats. Center wells: Paragonimus antigen(=PA) Outer wells: Cat serum collected at week interval (C: Control serum before infection).


	Fig. 9 Precipitin reaction of Clonorchis infected rabbits and cat sera (R₁, R₂, R₃ and C₃₁, C₃₂, C₃₃) with Clonorchis antigen (CA) and Paragonimus antigen (PA).

Tables


	Table 1 Agar-gel precipitin reaction in cats infected with Paragonimus westermani


	Table 2 Agar-gel precipitin reaction between the Clonorchis sinensis infected cat and rabbit sera with Clonorchis antigen(CA) and Paragonimus antigen (PA)

References


1.	Chang HT, Wang CW, Yu CF, Hsu CF, Fang JC. Paragonimiasis; a clinical study of 200 adult cases. Chin Med J 1958;77(1):3–9.

2.	Choi WY, Lee WK, Lee OR. [Indirect Fluorescent Antibody Test For Diagnosis Of Paragonimiasis]. Korean J Parasitol 1975;13(2):152–158.

3.	Choi WY, Kimura K, Tsuji M. [Immunoelectrophoresis For Anthelmintics Evaluation Against Experimental Paragonimiasis]. Korean J Parasitol 1976;14(2):94–102.

4.	Choi WY, Lee OR. [Immunoelectrophoresis for Fasciola hepatica]. Korean J Parasitol 1979;17(1):73–80.

5.	Choi WY, Lee OR. [Immunoelectrophoretic Studies Of Several Helminths]. Korean J Parasitol 1979;17(2):147–153.

6.	Han SH, Woo CK. [Immuno-Diffusion And Complement-Fixation Test In Paragonimiasis Of Cat]. Korean J Parasitol 1969;7(3):160–165.

7.	Katamine D, et al. Trop Med 1968;10(1):28–38.

8.	Kim JW, et al. J Med Center 1964;5:3–4.

9.	Seed JR, et al. J Parasit 1966;52(2):358–362.

*10.*	Yogore MG, et al. Am J Trop Med Hyg 1965;14(4):586–591.

*11.*	Yun DJ. Paragonimiasis in children in Korea, related to the custom of ingesting raw crayfish for the treatment of measles. J Pediatr 1960;56:736–751.