Fine structure of <i>Cysticercus celluosae</i> from human brain

, Suk, Jong Sik; , Sim, Bo Sung; , Lee, Soon Hyung

doi:1980.18.1.1

Korean J Parasito > Volume 18(1):1980 > Article

Original Article


Korean J Parasitol. 1980 Jun;18(1):1-14. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1980.18.1.1
Copyright © 1980 by The Korean Society for Parasitology


Fine structure of Cysticercus celluosae from human brain
Jong Sik Suk,^*Bo Sung Sim and Soon Hyung Lee
Department of Neurosurgery, College of Medicine, Seoul National University, Korea.
Department of Parasitology, College of Medicine, Chung-Ang University, Korea.


Abstract
A transmission electron microscopic study was conducted in order to know basic tegumental structure of Cysticercus cellulosae which was obtained from the ventricles of human brain. In general, the electron microscopic appearance of the tegument resembled that of the body wall of adult and larval forms of other cestodes. The tegument both of the parenchymatous portion (scolex and spiral canal) and bladder portion consisted of such components , i.e., an outer vesicular layer with microtriches, and an inner fibrous layer. The subtegumental tissue below the fibrous layer was filled with muscle bundles and tegumental cells. The surface of the larva was covered by the microtriches of two distictly different types. The characteristic pyramid-shaped "tetrahedral" form was observed on the surface of the scolex portion, whereas the elongated, slender "filamentous" microtriches were distributed on the regions of the spiral canal and bladder portion. The tegumental and subtegumental tissues varied in thickenss from one region to the next. The possibility of application in making differential diagnosis from other larval cestodes and possible fuction of this larval tegument were discussed.

Figures


	Fig. 1 Transmission electron micrographs of the body wall of Cysticercus cellulosae from human brain The scolex portion showing numerous microtriches, vesicular layer, fibrous layer and longitudinal muscles, tegumental cell and parenchyma. ×2,000


	Fig. 2 Transmission electron micrographs of the body wall of Cysticercus cellulosae from human brain Section through the spiral canal. Note the different type of microtriches with that of the scolex, ×5,000


	Fig. 3 Transmission electron micrographs of the body wall of Cysticercus cellulosae from human brain The bladder portion with scanty muscle bundles and rather thin vesicular layer. The shape of microtriches is similar with Fig. 2. ×3,000


	Fig. 4 Transmission electron micrographs of the body wall of Cysticercus cellulosae from human brain Higher magnification of small segment of the scolex. This shows the arrangement of tegumental and subtegumental tissues. Note cytoplasmic extension, fibrous layer, parenchyma and well developed muscle bundles. ×12,000


	Fig. 5 The microtriches on the surface of scolex. These show characteristic elongated "tetrahedral" pyramid-shaped microtriches. Longitudinal section through microtriches showing characteristic feature. The distal cytoplasm also showing numerous vesicles of electron-dense material and mitochondria (arrows). ×5,000


	Fig. 6 The microtriches on the surface of scolex. These show characteristic elongated "tetrahedral" pyramid-shaped microtriches. Higher magnification of Fig. 5. revealing wider proximal part and thinner electron-dense distal part of the characteristic microtriches. There is another dense region (arrow) which is continuous down to the vesicular layer. ×12,000


	Fig. 7 The microtriches on the surface of scolex. These show characteristic elongated "tetrahedral" pyramid-shaped microtriches. Cross sections of microtriches of the scolex. Note triangular cut-surfaces, which suggest their stereo scopic structures to a "tetrahedral" pyramid shaped feature. Numerous vesicles, granules of electron-dense material and mitochondria are also visible. ×3,000


	Fig. 8 The microtriches on the surface of scolex. These show characteristic elongated "tetrahedral" pyramid-shaped microtriches. Higher magnification of Fig. 7. Cross section of distal part is electron-dense, while that of proximal part shows peripheral dense region and inner lucid medullary region. ×12,000


	Fig. 9 The microtriches on the surface of the bladder portion. In comparison with those of the scolex, the microtriches of this portion show slender, longer and cylindrical "filamentous" appearance. Longitudinal section through the microtriches. The shorter proximal part carries much longer electron dense "filamentous" distal part. ×5,000 & ×8,000


	Fig. 10 The microtriches on the surface of the bladder portion. In comparison with those of the scolex, the microtriches of this portion show slender, longer and cylindrical "filamentous" appearance. Longitudinal section through the microtriches. The shorter proximal part carries much longer electron dense "filamentous" distal part. ×5,000 & ×8,000


	Fig. 11 The microtriches on the surface of the bladder portion. In comparison with those of the scolex, the microtriches of this portion show slender, longer and cylindrical "filamentous" appearance. Higher magnification of "filamentous" microtriches. The electron-dense region inside the proximal part is also observable. ×12,000


	Fig. 12 The microtriches on the surface of the bladder portion. In comparison with those of the scolex, the microtriches of this portion show slender, longer and cylindrical "filamentous" appearance. Cross section of "filamentous" microtriches. Note circular cut-surfaces which support cylindrical stereoscopic structure of proximal part. ×12,000

Abbreviations


E	Cytoplasmin extension
F	Fibrous layer
M	Muscle layer
m	Microtriches
b	Proximal part
d	Distal part
P	Parenchyma
T	Tegumental cell
V	Vesicular layer

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