Cytochemical demonstrations of lipase and nucleic acid in Trichomonas vaginalis were attempted. Modified Gomori tween method for lipase and acridine orange method for nucleic acid was applied. Trichomonas vaginalis incubated in the isolation mediurn (C.P.L.M.) were pooled and fixed using 0.1 N McIlvaine buffer saline and cold acetone for lipase and Walpole acetate buffer saline and acetic alcohol for nucleic acid. The results were obtained as follows: Activity of lipase and nucleic acid were recognized in the cytoplasm of T. vaginalis as scattered positive granules stained in blue yellow-green bright reddish color respectively. However these reactions were not shown in nucleus, nuclear membrane, undulating membrane etc. Present authos believe the negative finding of acridine orange staining in nucleus should be studied further.
