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Measurement of blood loss using 51Cr in heavy infection cases of Trichocephalus trichiurus
Seung Yull Cho
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.
Abstract
This study was undertaken to find out the possibility of iron-deficiency anemia by the intestinal blood loss in massive infection cases of T. trichiurus. Among the children of a community in which the whipworm infections were highly prevalent, 7 cases were selected whose E.P.G. of whipworm ova were more than 1,000. The hematological findings and estimated blood loss using 51Cr were undertaken. The results could be summarized as follows:
1) The E.P.G. and E.P.D. of T. trichiurus ova in studied 7 cases were 10,500-37,200/gm of feces and 0.90-5.14 million/day respectively.
2) Findings of hematological examination were in lower limit of normal in hemoglobin content and hematocrit. And erythrocytes were neither microcytic nor hypochromic. Differential count of leucocytes showed persistently low grade of eosinophilia.
3) Serum iron content measured by modification of Barkan method showed subnormal value in 5 out of 7 cases.
4) Erythrocyte radioactivity half disappearance time(T½) and intestinal blood loss were estimated using 51Cr-tagging erythrocytes. And the results showed that T½ were shortened (13-19 days ) in all cases and the blood loss was 0.94-6.54 ml/day which were correlated with worm burden (E.P.D.) significantly.
5) From the above results, it could be concluded that intestinal blood loss of T. trichiurus heavy infection were actually occurred but the amount of losses were easily compensated.
Figures
Fig. 1 Relation between E.P.D of T. trichiurus and daily blood loss measured by 51Cr.
Tables
Table 1 Result of egg counting by Stoll's method in this study
Table 2 Result of haematological examinations and serum iron contents
Table 3 Result of erythrocyte radioactivity half disappearence time (=T½) and blood loss
References
1.
Barkan G, et al. J Biol Chem 1940;135:37–42.
2.
Beck JE, et al. Abstr 1968;37(1):1–26.
3.
Burrows RB. On the estimation of Trichuris worm burdens in patients. J Parasitol 1950;36(3):227–231.
4.
Burrows RB, Lillis WG. The Whipworm as a Blood Sucker. J Parasitol 1964;50:675–680.
5.
Chandler AC. J Parasit 1930;16:198–206.
6.
Chitwood BG, et al. J Wash Acad Sci 1937;27:517–531.
7.
Fernan-Nunez M. Arch Int Med 1927;40:46–57.
8.
Getz L. Am J Dis Child 1945;70:19–24.
9.
Gray SJ, et al. J Cl Invest 1950;29:1604–1613.
10.
Jung RC, Beaver PC. Clinical observations on Trichocephalus trichiurus (whipworm) infestation in children. Pediatrics 1951;8(4):548–557.
11.
Kikuchi S, et al. Jpn J Parasit 1964;13:11–24.
12.
Layrisse M, Aparcedo L, Martinez-Torres C, Roche M. Blood loss due to infection with Trichuris trichiura. Am J Trop Med Hyg 1967;16(5):613–619.
13.
Lee M, et al. Korean J Nuclear Med 1967;1:55–66.
14.
Mahmood A. Blood loss caused by helminthic infections. Trans R Soc Trop Med Hyg 1966;60(6):766–769.
15.
Miller MJ. Canadian J Res Sect D 1947;25:1–11.
16.
Otto GF. Am J Trop Med 1935;15:693–704.
17.
Roche M, Perez-Gimenez ME, Layrisse M, Di Prisco ME. Study of urinary and fecal excretion of radioactive chromium Cr51 in man; its use in the measurement of intestinal blood loss associated with hookworm infection. J Clin Invest 1957;36(7):1183–1192.
18.
Seo BS, Rim HJ, Loh IK, Lee SH, Cho SY, Park SC, Bae JW, Kim JH, Lee JS, Koo BY, Kim KS. [Study On The Status Of Helminthic Infections In Koreans]. Korean J Parasitol 1969;7(1):53–70.
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