The Trichomonas immobilization-agglomeration reaction was studied using the sera from women with vaginal trichomoniasis and from rabbits inoculated with cultures of T. vaginalis. It was found that the greatest amount of immobilization occurred at 25 to 30 minutes, and that inactivation of the sera did not affect the results. An evidence of antigenic differences between T. vaginalis, T. hominis and C. albicans was confirmed. The immobilization reaction was positive in 80.3% of the 71 T. vaginalis positive cases, but in only 7.7% of the 77 persons of T. vaginalis negative cases. From the above results, it is suggested that immobilization reaction is a useful tool for the diagnosis of T. vaginalis infection.
