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Original Article

Fishborne Trematode Metacercariae Detected in Freshwater Fish from Vientiane Municipality and Savannakhet Province, Lao PDR

Han-Jong Rim1, Woon-Mok Sohn2, Tai-Soon Yong3, Keeseon S. Eom4, Jong-Yil Chai5, Duk-Young Min6, Soon-Hyung Lee7, Eui-Hyug Hoang7, Bounlay Phommasack8, Sithat Insisengmay8
The Korean Journal of Parasitology 2008;46(4):253-260.
Published online: December 20, 2008

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8Department of Hygiene and Prevention, Ministry of Public Health, Vientiane Lao PDR.

Corresponding author (wmsohn@gnu.ac.kr)
• Received: August 16, 2008   • Accepted: November 5, 2008

Copyright © 2008 by The Korean Society for Parasitology

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Fishborne Trematode Metacercariae Detected in Freshwater Fish from Vientiane Municipality and Savannakhet Province, Lao PDR
Korean J Parasitol. 2008;46(4):253-260.   Published online December 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.4.253
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Fishborne Trematode Metacercariae Detected in Freshwater Fish from Vientiane Municipality and Savannakhet Province, Lao PDR
Korean J Parasitol. 2008;46(4):253-260.   Published online December 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.4.253
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Fishborne Trematode Metacercariae Detected in Freshwater Fish from Vientiane Municipality and Savannakhet Province, Lao PDR
Image Image Image Image Image
Fig. 1 Surveyed areas, Vientiane Municipality (◉) and Savannakhet Province (○), in Lao PDR.
Fig. 2 (A) Opisthorchis viverrini metacercaria detected in a freshwater fish from Lao PDR. Scale bar = 75 µm. (B) An excysted O. viverrini metacercaria which has nearly equal sized 2 suckers (OS: oral sucker; VS: ventral sucker), brownish pigment scattered in the body, and a round excretory bladder (EB) in the posterior portion. Scale bar = 150 µm.
Fig. 3 (A) Haplorchis taichui metacercaria detected in a freshwater fish from Lao PDR. Scale bar = 75 µm. (B) Enlarged view of the A ventrogenital sac (arrow in A) which is baseball glove shaped.
Fig. 4 (A) Haplorchis yokogawai metacercaria detected in a freshwater fish from Lao PDR. Scale bar = 75 µm. (B) Enlarged view of the A ventrogenital sac (arrow in A) which has numerous minute spines.
Fig. 5 (A) Centrocestus formosanus metacercaria detected in a freshwater fish from Lao PDR. Scale bar = 50 µm. (B) Enlarged view of the oral sucker A armed with about 32 circumoral spines.

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Fishborne Trematode Metacercariae Detected in Freshwater Fish from Vientiane Municipality and Savannakhet Province, Lao PDR
ST category No. of fishexamined Length (cm)
Weight (g)
Range Average Range Average Cyprinidae  Puntius brevis 54 3.5-9.0 6.5 1.0-10.4 5.6  Rasbora tornieri 16 4.0-6.5 5.4 1.0-4.0 2.0  Puntioplites falcifer 14 4.3-13.0 7.6 12.0-16.0 15.1  Hampala dispar 11 10.5-13.0 10.5 14.0-40.0 21.3  Esomus metallicus 10 4.5-6.0 5.0 1.0-3.0 1.7  Sikukia gudgeri 7 7.0-10.0 8.4 6.0-10.0 7.0  Cyclocheilichthys apogon 6 5.0-12.5 11.8 19.0-34.0 25.0  Cyclocheilichthys armatus 6 7.6-9.9 8.5 10.1-22.9 14.5  Rasbora myersi 5 8.0-11.0 10.2 6.0-17.0 11.4  Rasbora dusonensis 5 6.5-8.5 7.5 - -  Thynnichthys thynnoides 5 9.0-10.5 9.8 - -  Mystacoleucus marginatus 4 6.5-8.5 7.6 4.0-8.0 6.0  Osteochilus hasseltii 3 11.5-12.0 11.9 22.0-37.0 27.7  Osteochilus lini 2 7.0-9.5 8.3 - - Ambassidae  Parambassis apogonoides 4 - 3.0 - 1.0 Anabantidae  Anabas testudineus 2 5.0-8.0 6.5 4.0-12.0 8.0 Mastacembelidae  Macrognathus siamensis 2 - 13.0 - 15.0 Species of fish No. of fishexamined Length (cm)
Weight (g)
Range Average Range Average Cyprinidae  Cyclocheilichthys repasson 35 5.0-7.5 6.0 3.1-9.1 5.3  Onychostoma elongatum 34 6.7-12.0 8.4 7.7-38.2 12.2  Puntius brevis 25 5.6-6.2 5.9 2.2-5.6 3.6  Cyclocheilichthys armatus 20 6.2-13.5 8.0 4.5-44.2 13.6  Hampala dispar 18 5.7-12.8 10.7 4.2-51.2 35.8  Labiobarbus leptocheila 11 7.0-8.7 7.9 5.6-11.3 8.8  Osteochilus hasseltii 10 6.0-9.0 7.5 5.8-17.8 10.4  Cirrhinus molitorella 8 5.0-7.2 6.4 2.8-9.1 6.6  Hypsibarbus wetmorei 4 11.5-18.0 13.9 45.8-164.0 84.2  Hypsibarbus lagleri 2 5.5-10.0 7.8 4.7-27.9 16.3 Anabantidae  Anabas testudineus 5 4.5-7.2 6.5 7.8-18.0 16.2 Osphronemidae  Trichogaster microlepis 5 - - - 4.5 Species of fish No. of fish examined No. (%) of fish infected No. of metacercariae detected
Total Range Average Puntius brevis 25 23 (92.0) 8,274 24-1,227 360 29 25 (86.2) 10-120a Hampala dispar 11 8 (72.7) 5-10a Puntioplites falcifer 9 3 (33.3) 4 1-2 1.3 Esomus metallicus 8 6 (75.0) 3-15a Cyclocheilichthys armatus 6 6 (100) 11,939 8-4,030 1,990 Mystacoleucus marginatus 4 2 (50.0) 2-5a Species of fish No. of fish examined No. (%) of fish infected No. of metacercariae detected
Total Range Average Puntius brevis 25 2 (8.0) 9 3-6 4.5 Puntioplites falcifer 9 2 (22.2) 2 1a 1.0 5 2 (40.0) Mystacoleucus marginatus 4 1 (25.0) 1a Species of fish No. of fish examined No. (%) of fish infected No. of metacercariae detected
Total Range Average Onychostoma elongatum 9 4 (44.4) 9,761 5-9,500 2,440 25 - 7,768 - - Cyclocheilichthys armatus 20 20 (100) 12,936 47-6,980 647 Hampala dispar 13 13 (100) 5,272 21-1,958 406 5 - 408 - - Puntius brevis 25 - 646 - - Cyclocheilichthys repasson 10 8 (80.0) 16 1-6 2.0 25 - 100 - - Osteochilus hasseltii 10 2 (20.0) 2 - 1.0 Hypsibarbus lagleri 2 1 (50.0) 115 - 115 Species of fish No. of fish examined No. (%) of fish infected No. of metacercariae detected
Total Range Average Cyclocheilichthys repasson 10 6 (60.0) 62 1-27 10.3 25 - 21 - - Onychostoma elongatum 9 4 (44.4) 26 3-15 6.5 25 - 22 - - Cyclocheilichthys armatus 20 2 (10.0) 35 1-34 17.5 Hampala dispar 13 4 (30.8) 7 1-2 1.8 Labiobarbus leptocheila 11 8 (72.7) 20 1-5 2.5 Cirrhinus molitorella 8 1 (12.5) 1 - 1.0 Species of fish No. of fish examined No. (%) of fish infected No. of metacercariae detected
Total Range Average Cyclocheilichthys repasson 10 1 (10.0) 5 - 5.0 25 - 6 - - Onychostoma elongatum 9 6 (66.7) 434 2-214 72.0 20 - 3,314 - - Cyclocheilichthys armatus 20 20 (100) 7,090 8-1,370 355 Hampala dispar 13 9 (69.2) 2,012 6-522 224 Labiobarbus leptocheila 11 7 (63.6) 223 2-112 40 Osteochilus hasseltii 10 5 (50.0) 15 1-7 3.0 Cirrhinus molitorella 8 2 (25.0) 4 1-3 2.0 Hypsibarbus wetmorei 4 3 (75.0) 279 61-123 93 Hypsibarbus lagleri 2 2 (100) 246 10-236 123 Species of fish No. of fish examined No. (%) of fish infected No. of metacercariae detected
Total Range Average Cyclocheilichthys repasson 10 2 (20.0) 7 3-4 3.5 25 - 18 - - Puntius brevis 25 - 713 - - Osteochilus hasseltii 10 4 (40.0) 39 1-35 9.8 Cirrhinus molitorella 8 2 (25.0) 15 4-11 7.5
Table 1. Freshwater fishesa collected from Savannakhet Province, Lao PDR

A total of 156 fish of 17 species were examined.

Table 2. Freshwater fisha collected from Vientiane Municipality, Lao PDR

A total of 177 fish of 12 species were examined.

Table 3. Infection status of fish with Opisthorchis viverrini metacercariae in Savannakhet Province

No. of metacercariae per a gram of fish flesh.

Table 4. Infection status of fish with Haplorchis taichui metacercariae in Savannakhet Province

No. of metacercariae per a gram of fish flesh.

Table 5. Infection status of fish with Opisthorchis viverrini metacercariae in Vientiane Municipality
Table 6. Infection status of fish with Haplorchis taichui metacercariae in Vientiane Municipality
Table 7. Infection status of fish with Haplorchis yokogawai metacercariae in Vientiane Municipality
Table 8. Infection status of fish with Centrocestus formosanus metacercariae in Vientiane Municipality

Table 1.

Table 2.

Table 3.

Table 4.

Table 5.

Table 6.

Table 7.

Table 8.

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