Studies on the larval trematodes from brackish water crabs. Studies on a kind of <i>Levinseniella</i>

Original Article


Korean J Parasitol. 1965 Jun;3(1):31-38. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1965.3.1.31
Copyright © 1965 by The Korean Society for Parasitology


Studies on the larval trematodes from brackish water crabs. Studies on a kind of Levinseniella
Dong Wik Choi, M.D.,Sang Whon Lee, M.D.,Jung Man Kim, M.D. and Jong Koo Kim, M.D.
Department of Preventive Medicine, School of Medicine, Kyungpook National University, Korea.


Abstract
1. Metacercariae of a kind of Levinseniella species were found from a brackish water crabs, Macrophthalmus japonicus de Haan, in the brackish area of Hyungsan river of Kyungpook province. 2. Habitats were the epigastric and progastric regions of Macrophthalmus japonicus. 3. One hundred and seventy five crabs out of 178 examined (98.3 per cent) were infested with metacercariae of Levinseniella species. The infested number of metacercariae in each crabs was average 100 ea. 4. The excystation of metacercaria has not developed in vitro (both saline and tyrode's solution). 5. Many eggs were observed in the metacercariae maintained for 24 or 48 hours in the water-bath. 6. No differences were observed between semi-adult fluke from rat host and those maintained for 24 or 48 hours in vitro.

Figures


	Fig. 1 Geographical illustration of brackish area where crabs were collected.


	Fig. 2 Macrophthalmus japonicus, dorsal view.


	Fig. 3 Macrophthalmus japonicus, ventral view.


	Fig. 4 Metacercarias in low power magnification (100×)


	Fig. 5 Metacercaria (400×) Note excretory bladder and seminal vesicle.


	Fig. 6 Twined cystic wall of metacercaria. (100×)


	Fig. 7 Excysted metacercaria, Iron-Hematoxylin stained view. (100×)


	Fig. 8 Excysted metacercaria. Note alreaady formed eggs.


	Fig. 9 Semi-adult fluke recovered from rat host which was collected 24hrs. after infection. 50~100 ea. eggs are visible in the uterus.


	Fig. 10 Semi-adult fluke recovered from rat host which was collected 48 hrs after infection. 200~350 ea. eggs are visible in the uterus.


	Fig. 11 Encysted metacerearia maintained for 48hrs. in vitro(Tyrode's solution). 200~500 ea. eggs are observable in the encysted larva.


	Fig. 12 Semi-adult fluke.


	Fig. 13 Egg of a kind of Levinseniella. (100×)

Tables


	Table 1 Size of encysted metacercarias(mm).


	Table 2 Size of encysted metacercarias(mm).


	Table 3 Results of distribution of excysted worms in gastrointestinal tract of rats and young chickens.


	Table 4 Measurements of 5 semi-adult Levinseniella species maintained in vitro and vivo (Iron-Hematoxylin stained specimens).

References


5.	Miyazaki I. Fukuoka Acta Medica 1939;32(5):887–896.

8.	Miyakawa M, et al. Medicine and Biology 1955;35(1):14–17.

9.	Miyakawa M, et al. Medicine and Biology 1955;35(1):25–28.

*10.*	Nakase M. Shikoku Acta Medica 1958;13(4):494–506.

*11.*	Nakase M. Shikoku Acta Medica 1958;13(4):507–519.

*12.*	Nakase M. Shikoku Acta Medica 1958;13(4):520–527.

*13.*	Otagaki H. Jap J Parast 1958;7(2):167–182.

*15.*	Seo BS, Yoon JS, Lee CW, Lee SH. Studies On Microphalloides Japonicus. Korean J Parasitol 1964 Jun;2(1):41–51.