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Original Article

A New IgG Immunoblot Kit for Diagnosis of Toxoplasmosis in Pregnant Women

Imen Khammari1, Fatma Saghrouni1, Sami Lakhal2, Aida Bouratbine2, Moncef Ben Said1, Jalel Boukadida1
The Korean Journal of Parasitology 2014;52(5):493-499.
Published online: October 22, 2014

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Corresponding author (imenkhammari@yahoo.fr)
• Received: April 23, 2014   • Revised: June 12, 2014   • Accepted: June 15, 2014

© 2014, Korean Society for Parasitology and Tropical Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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A New IgG Immunoblot Kit for Diagnosis of Toxoplasmosis in Pregnant Women
Korean J Parasitol. 2014;52(5):493-499.   Published online October 22, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.5.493
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A New IgG Immunoblot Kit for Diagnosis of Toxoplasmosis in Pregnant Women
Korean J Parasitol. 2014;52(5):493-499.   Published online October 22, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.5.493
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A New IgG Immunoblot Kit for Diagnosis of Toxoplasmosis in Pregnant Women
Image Image Image
Fig. 1 Frequencies of antigenic bands recognized by IgG antibodies in positive (Group I) and negative sera (Group II). *Antigenic bands with a relevant Youden Index are indicated with an asterisk.
Fig. 2 Patterns shown by equivocal and/or discordant sera (Group III) tested in LDBIO Toxo II IgG®. Lane 1: positive control with the 45, 40, 33, 31, and 30 kDa. Lane 2: negative sera with the 30 kDa. Lane 3: positive sera with the 30, 31, and 33 kDa. Lane 4: negative sera with the 30 and 31kDa. Lane 5: negative sera with the 30 and 31 kDa. Lane 6: positive sera with the 30, 31, 40, and 45 kDa. Lane 7: sera with the 30 kDa. Lane 8: positive sera with the 30, 31, and 33 kDa. Lane 9: positive sera with the 30, 31, and 33 kDa. Lane 10: negative sera with the 30 and 31 kDa. Lane 11: negative sera with the 30 kDa.
Fig. 3 Patterns shown by equivocal and discordant sera (Group III) tested in our homemade immunoblot. Lane A: molecular weight marker. Lane 1: negative sera with the 36 kDa. Lane 2: negative sera with the 36 kDa. Lane 3: negative sera with the 36. Lane 4: negative sera. Lane 5: negative sera. Lane 6: negative sera with the 36 kDa. Lane 7: negative sera with the 36 and 33 kDa. Lane 8: negative sera with the 33 and 21 kDa. Lane 9: positive sera with the 36, 33, and 21 kDa. Lane 10: positive sera with the 98, 36, 33, 32, and 21 kDa.

Fig. 1

Fig. 2

Fig. 3

A New IgG Immunoblot Kit for Diagnosis of Toxoplasmosis in Pregnant Women
Bands kDa Positive sera (n = 154) Negative sera (n = 100) Sensitivity CI 95% Specificity CI 95% PPV CI 95% NPV CI 95% YI 98 128 13 83.1 (76.1, 88.5) 87.0 (78.4, 92.6) 90.8 (84.4, 94.8) 77.0 (67.9, 84.2) 0.701 36 146 11 94.8 (89.7, 97.6) 89.0 (80.8, 94.1) 93.0 (87.5, 96.3) 91.8 (83.9, 96.1) 0.838 33 112 7 72.7 (64.9, 79.4) 93.0 (85.6, 96.9) 94.1 (87.8, 97.4) 68.9 (60.3, 76.4) 0.657 32 144 8 93.5 (88.1, 96.7) 92.0 (84.4, 96.2) 94.7 (89.5, 97.5) 90.2 (82.3, 94.9) 0.855 21 125 12 81.2 (73.9, 86.8) 88.0 (79.6, 93.4) 91.2 (84.9, 95.2) 75.2 (66.2, 82.5) 0.692 Positive sera (n = 154) Negative sera (n = 100) Sensitivity (CI 95%) Specificity (CI 95%) PPV (CI 95%) NPV (CI 95%) YI Presence of at least 1 band 154 36 100.0 (97.0, 100.0) 64.0 (53.7, 73.2) 81.1 (74.0, 86.2) 100.0 (92.9, 100.0) 0.640 Presence of at least 2 bands 153 14 99.4 (95.9, 100.0) 86.0 (77.3, 91.9) 91.6 (86.1, 95.2) 98.9 (92.9, 99.9) 0.854 Presence of a least 3 bands 150 1 97.4 (93.1, 99.2) 99.0 (93.8, 99.9) 99.3 (95.8, 100.0) 96.1 (89.8, 98.7) 0.964 Presence of a least 4 bands 129 0 83.8 (76.8, 89.0) 100.0 (95.4, 100.0) 100.0 (96.4, 100.0) 80.0 (71.7, 86.4) 0.838 Simultaneous presence of 5 bands 69 0 44.8 (36.9, 53.0) 100.0 (93.4, 100.0) 100.0 (93.4, 100.0) 54.1 (46.6, 61.3) 0.448
Table 1. Intrinsic characteristics of the 5 relevant bands (98, 36, 33, 32, and 21 kDa) selected in our home-made immunoblot test

CI, confidence interval; PPV, positive predictive value; NPV, negative predictive value; YI, youden index.

Table 2. Intrinsic characteristics of the different profiles obtained with the 5 relevant bands (98, 36, 33, 32, and 21 kDa) detected in our immunoblot test

CI, confidence interval; PPV, positive predictive value; NPV, negative predictive value; YI, youden index.

Table 1.

Table 2.

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