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Brief Communication

Differential Effects of Two Widely Used Solvents, DMSO and Ethanol, on the Growth and Recovery of Trypanosoma cruzi Epimastigotes in Culture

The Korean Journal of Parasitology 2017;55(1):81-84.
Published online: February 28, 2017

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*Corresponding author (robertohf@biomedicas.unam.mx)
• Received: September 6, 2016   • Revised: December 15, 2016   • Accepted: January 3, 2017

Copyright © 2017 by The Korean Society for Parasitology and Tropical Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Differential Effects of Two Widely Used Solvents, DMSO and Ethanol, on the Growth and Recovery of Trypanosoma cruzi Epimastigotes in Culture
Korean J Parasitol. 2017;55(1):81-84.   Published online February 28, 2017
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Differential Effects of Two Widely Used Solvents, DMSO and Ethanol, on the Growth and Recovery of Trypanosoma cruzi Epimastigotes in Culture
Korean J Parasitol. 2017;55(1):81-84.   Published online February 28, 2017
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Differential Effects of Two Widely Used Solvents, DMSO and Ethanol, on the Growth and Recovery of Trypanosoma cruzi Epimastigotes in Culture
Image Image
Fig. 1 Effects of ethanol and DMSO on T. cruzi growth. The growth of T. cruzi epimastigotes was periodically monitored for 9 days, as indicated. All cultures were started at an initial cellular concentration of 1×106 parasites/ml. LIT medium without solvent was used as a control. (A) Growth curves of epimastigotes cultured in different ethanol concentrations, as indicated. (B) Growth curves of epimastigotes cultured in different DMSO concentrations, as indicated. Plotted values represent the arithmetic means from 3 independent experiments. (C, D) Final cellular density of 9 day-old epimastigotes cultured in the presence of different amounts of the indicated solvents, relative to the solvent-free LIT growth controls.
Fig. 2 Growth recovery of T. cruzi epimastigotes after treatment with either ethanol (A) or DMSO (B). Nine day-old epimastigote cultures, pre-grown in the presence of the indicated solvents, were washed with PBS and placed in solvent-free LIT medium at an adjusted cellular density of 1 million parasites per ml. Control cultures of cells previously grown in LIT medium were also included. Cellular growth was monitored at the indicated times. Plotted values represent the means of at least 2 independent experiments.
Differential Effects of Two Widely Used Solvents, DMSO and Ethanol, on the Growth and Recovery of Trypanosoma cruzi Epimastigotes in Culture