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Original Article

Characterization of a Toxocara canis species-specific excretory-secretory antigen (TcES-57) and development of a double sandwich ELISA for diagnosis of visceral larva migrans

R.D. Iddawela1, R.P.V.J. Rajapakse2, N.A.N.D. Perera2, Takeshi Agatsuma3
The Korean Journal of Parasitology 2007;45(1):19-26.
Published online: March 20, 2007

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Corresponding author (agatsuma@kochi-u.ac.jp)
• Received: March 22, 2006   • Accepted: January 4, 2007

Copyright © 2007 by The Korean Society for Parasitology

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Characterization of a Toxocara canis species-specific excretory-secretory antigen (TcES-57) and development of a double sandwich ELISA for diagnosis of visceral larva migrans
Korean J Parasitol. 2007;45(1):19-26.   Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.19
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Characterization of a Toxocara canis species-specific excretory-secretory antigen (TcES-57) and development of a double sandwich ELISA for diagnosis of visceral larva migrans
Korean J Parasitol. 2007;45(1):19-26.   Published online March 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.1.19
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Characterization of a Toxocara canis species-specific excretory-secretory antigen (TcES-57) and development of a double sandwich ELISA for diagnosis of visceral larva migrans
Image Image Image
Fig. 1 Pattern of bands produced by antigens and molecular marker, separated on SDS-PAGE and stained with Coomassie blue. TcES, Toxocara canis ES antigen; AlES, Ascaris lumbricoides ES antigen; TvES, Toxocara vitulorum ES antigen; NaL, Necator americanus larval antigen. MW: molecular weight marker (high molecular weight range).
Fig. 2 Western blot of antigens against anti TcES rabbit sera. TcES, T. canis ES antigen; AlES, A. lumbricoides ES antigen; TvES, T. vitulorum ES antigen; NaL, N. americanus larval antigen.
Fig. 3 Western blot of antigens against toxocariasis positive human serum and anti TcES-57 rabbit serum. TcES, T. canis ES antigen; AlES, A. lumbricoides ES antigen; TvES, T. vitulorum ES antigen; NaL, N. americanus larval antigen.

Fig. 1

Fig. 2

Fig. 3

Characterization of a Toxocara canis species-specific excretory-secretory antigen (TcES-57) and development of a double sandwich ELISA for diagnosis of visceral larva migrans
PHD : Parasites, Hosts and Diseases

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