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Original Article

Sirtinol Supresses Trophozoites Proliferation and Encystation of Acanthamoeba via Inhibition of Sirtuin Family Protein

So-Young Joo1orcid, Ja Moon Aung1, Minsang Shin2, Eun-Kyung Moon3orcid, Hyun-Hee Kong4orcid, Youn-Kyoung Goo1, Dong-Il Chung1, Yeonchul Hong1,*orcid
The Korean Journal of Parasitology 2022;60(1):1-6.
Published online: February 23, 2022

1Department of Parasitology and Tropical Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Korea

2Department of Microbiology, School of Medicine, Kyungpook National University, Daegu 41944, Korea

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*Corresponding author (ychong@knu.ac.kr)
• Received: December 8, 2021   • Revised: January 17, 2022   • Accepted: January 27, 2022

© 2022, Korean Society for Parasitology and Tropical Medicine

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Sirtinol Supresses Trophozoites Proliferation and Encystation of Acanthamoeba via Inhibition of Sirtuin Family Protein
Korean J Parasitol. 2022;60(1):1-6.   Published online February 23, 2022
DOI: https://doi.org/10.3347/kjp.2022.60.1.1
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Sirtinol Supresses Trophozoites Proliferation and Encystation of Acanthamoeba via Inhibition of Sirtuin Family Protein
Korean J Parasitol. 2022;60(1):1-6.   Published online February 23, 2022
DOI: https://doi.org/10.3347/kjp.2022.60.1.1
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Sirtinol Supresses Trophozoites Proliferation and Encystation of Acanthamoeba via Inhibition of Sirtuin Family Protein
Image Image
Fig. 1 Effects of sirtinol on the proliferation and encystation of A. castellanii trophozoites. (A) Trophozoites were incubated with various concentrations of sirtinol or DMSO (control). For each sirtinol or DMSO concentration, the data represent the mean cell number at 48 hr after incubation. (B) Effects of sirtinol on A. castellanii trophozoite encystation. A. castellanii trophozoites were transferred into an encystation medium containing 200-μM sirtinol and incubated for 24 hr. The mature cysts were counted under a microscope, followed by treatment with sarkosyl. DMSO was used as solvent control. (C) Transcriptional changes in CSCP in encysting AcSir2-overexpressing cells. AcSir2-overexpressing trophozoites (0 hr) and encysting cells at 24, 48, and 72 hr after encystation induction were examined for transcriptional changes in CSCP using qRT-PCR. CSCP transcriptional levels were normalized to that of Acanthamoeba actin. (D) Effects of sirtinol on transcription levels in CS and CSCP during the encystation process. Trophozoites were transferred into an encystation medium containing 100-μM sirtinol, incubated for 24 hr, examined for transcriptional changes in CS and CSCP using qRT-PCR, and compared to those of DMSO-treated cells. **P<0.01, ***P<0.001, and ****P<0.0001.
Fig. 2 Inhibition of A. castellanii encystation using sirtinol treatment. Ultrastructural changes following treatment with sirtinol in the encysting of A. castellanii. Encystation was induced by transferring cells into an encystation medium containing DMSO (A, D), 100 μM sirtinol (B, E), or salermide (C, F) for 24 hr. The encystation medium also received the same amount of DMSO (as a solvent control). The black arrowhead, empty arrowhead, and black double-headed arrows, and black arrows indicate the exocyst wall, endocyst wall, intercyst space, and autophagosome-like structures, respectively. Scale-bar: 1 μM.

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Sirtinol Supresses Trophozoites Proliferation and Encystation of Acanthamoeba via Inhibition of Sirtuin Family Protein
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