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ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera

Hye-Jin Ahn1, Sera Kim1, Dae-Yong Kim2, Ho-Woo Nam1
The Korean Journal of Parasitology 2003;41(3):175-177.
Published online: September 20, 2003

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Corresponding author (howoo@catholic.ac.kr)
• Received: July 3, 2003   • Accepted: July 30, 2003

Copyright © 2003 by The Korean Society for Parasitology

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ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera
Korean J Parasitol. 2003;41(3):175-177.   Published online September 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.3.175
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ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera
Korean J Parasitol. 2003;41(3):175-177.   Published online September 20, 2003
DOI: https://doi.org/10.3347/kjp.2003.41.3.175
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ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera
Image Image
Fig. 1 Cloning of genes from the C-terminal 2/3 fragment of Nc-p43 from Neospora caninum (Ncp43C) and from the N-terminal 2/3 fragment of SAG1 from Toxoplasma gondii (TgSAG1N) in pQE30 vector.
Fig. 2 SDS-PAGE of M15 strain transformed with Ncp43C (Ncp43P) and TgSAG1N (TgSAG1A) plasmids, which were expressed by IPTG induction (i). Ni-NTA column purified antigens were represented by 26 kDa and 19 kDa bands. Numerals on the left indicated molecular mass as kDa.

Fig. 1

Fig. 2

ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera
 Province No. examined No. positive (%) (N. caninum) No. positive (%) (T. gondii)
Gyeonggi-do 122 12 (9.8%) 0 (0%)
Gangwon-do 121 16 (13.2%) 0 (0%)
Chungcheongbuk-do 72 10 (13.9%) 0 (0%)
Chungcheongnam-do 120 10 (8.3%) 0 (0%)
Gyeongsangbuk-do 93 15 (16.1%) 0 (0%)
Gyeongsangnam-do 84 9 (10.7%) 0 (0%)
Jeollabuk-do 86 11 (12.8%) 0 (0%)
Jeollanam-do 120 20 (16.7%) 0 (0%)
Jeju-do 34 0 (0%) 0 (0%)
Total 852 103 (12.1%) 0 (0%)
Table 1. Seroprevalence of anti-Neospora caninum antibodies in cattle by IgG-ELISA using Ncp43P and TgSAG1A

Table 1.

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