Correlation of sonographic findings with histopathological changes of the bile ducts in rabbits infected with <i>Clonorchis sinensis</i>

, Hong, S T; , Park, K H; , Seo, M; , Choi, B I; , Chai, J Y; , Lee, S H

doi:1994.32.4.223

Korean J Parasito > Volume 32(4):1994 > Article

Original Article


Korean J Parasitol. 1994 Dec;32(4):223-230. English. Published online Dec 20, 1994. http://dx.doi.org/10.3347/kjp.1994.32.4.223
Copyright © 1994 by The Korean Society for Parasitology


Correlation of sonographic findings with histopathological changes of the bile ducts in rabbits infected with Clonorchis sinensis
S T Hong,¹K H Park,²M Seo,¹B I Choi,³J Y Chai,¹ and S H Lee^*¹
¹Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea.

Received October 10, 1994; Accepted November 21, 1994.
Abstract
Clonorchiasis is an important parasitic disease of humans in Korea. The present study intended to compare sonographic findings with histopathological changes in experimental clonorchiasis. Eighteen New Zealand white rabbits were infected with metacercariae of Clonorchis sinensis, and examined 4, 10, and 22 weeks post-infection (PI). Four infected rabbits were treated with praziquantel 10 weeks PI and were examined 12 weeks after treatment. Sonography revealed mild to severe dilatation of the intrahepatic ducts (IHDD) and slightly increased periductal echoes in 12 out of 14 rabbits at 4 weeks PI, and all of the animals after 10 and 22 weeks PI and 12 weeks after treatment. The histopathological lesions were duct dilatation, mucosal hyperplasia, and periductal fibrosis, which progressed from 4 weeks to 22 weeks PI and even in treated rabbits. The dilated intrahepatic ducts over 1 mm diameter were detected by sonography. The present results indicate that sonographic findings are well correlated with histopathological lesions in rabbit clonorchiasis except for early phase of light burden of infection. The sonography has a limitation in discriminating residual sequelae of the ducts after praziquantel treatment.

Figures

Figs. 1-2
Fig. 1A. Sonogram of a control rabbit liver. The portal vein branches (arrow heads) are visualized but bile ducts are not. Fig. 1B. Histopathological observation of the liver of the control rabbit, showing normal architecture of the liver and normal intrahepatic ducts. HE stain, original magnification × 60. Fig. 2A. sonogram of the rabbit 20-2 at 4 weeks PI. The portal vein (arrow heads) and bile ducts (arrows) were visualized. Fig. 2B. Sonogram of the rabbit 20-2. 10 weeks PI. showing moderate dilatation of the intrahepatic ducts (arrows) along the portal vein and increased periductal echoes.

Figs. 3-4
Fig. 3A. Sonogram of the 20-4 rabbit, 10 weeks PI. The ducts (arrows) are dilated moderately with periductal echoes. Fig. 3B. Photomicrograph of the rabbit 20-4, 10 weeks PI. The ducts (arrows) infected by flukes are remarkably dilated. Portal vein (arrow heads) is also seen nearby. HE stain, original magnification × 15. Fig. 4A. Sonogram of the 400-15 rabbit, 12 weeks after treatment. The ducts (arrows) are still dilated moderately with increased periductal echoes. Fig. 4B. Photomicrograph of the rabbit 400-15, 12 weeks after treatment. The ducts (arrows) are still dilated. Portal vein (arrow heads) is also seen nearby. HE stain, original magnification × 15.

Tables


	Table 1 Scheme of experimental infection of rabbits with Clonorchis sinensis


	Table 2 Sonographic findings of rabbits 4, 10 and 22 weeks after infection with C. sinensis


	Table 3 EPG and sonographic findings of rabbits 4, 10 and 22 weeks after infection with C. sinensis


	Table 4 Histopathological findings of rabbits infected with C. sinensis

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