Effects of cytokines in the activation of peritoneal macrophages from mice infected with <i>Toxoplasma gondii</i>

, Lee, Y H; , Shin, D W

doi:1994.32.3.185

Korean J Parasito > Volume 32(3):1994 > Article

Original Article


Korean J Parasitol. 1994 Sep;32(3):185-193. English. Published online Sep 20, 1994. http://dx.doi.org/10.3347/kjp.1994.32.3.185
Copyright © 1994 by The Korean Society for Parasitology


Effects of cytokines in the activation of peritoneal macrophages from mice infected with Toxoplasma gondii
Y H Lee,^* and D W Shin
Department of Parasitology, College of Medicine, Chungnam National University, Taejon 301-131, Korea.

Received July 08, 1994; Accepted July 22, 1994.
Abstract
The present study was undertaken to assess the role of cytokines in the activation of peritoneal macrophages from Toxoplasma-infected mice. Peritoneal macrophages from Toxoplasma-infected mice (10 cysts of Beverley strain/mouse) were harvested 8 weeks after infection, and incubated with the mitogen-induced lymphokine, recombinant mouse interferon-γ (IFN-γ), recombinant mouse tumor necrosis factor-α (TNF-α) alone or in combination with IFN-γ (IFN-γ/TNF-α) for 24 hr at 37℃, 5% CO₂. Macrophage activation was measured by the amount of H₂O₂ and NO₂- production, and anti-Toxoplasma activities of macrophages. IFN-γ or IFN-γ/TNF-α-treated macrophages from Toxoplasma-infected mice revealed significantly higher H₂O₂ production than resident macrophages from Toxoplasma-infected mice. The production of NO₂- by TNF-α-, IFN-γ- or IFN-γ/TNF-α-treated macrophages from Toxoplasma-infected mice were significantly higher than that by resident macrophages, whereas lymphokine-treated group produced similar amount as that produced by resident macrophages. Anti-Toxoplasma activities of cytokine-treated macrophages from Toxoplasma-infected mice were significantly higher than those of resident macrophages. IFN-γ-treated macrophages were significantly increased production of H₂O₂ and NO₂-, and anti-Toxoplasma activities of macrophages between normal and Toxoplasma-infected mice, whereas the other cytokine-treated groups were not significant differences between them. These data suggested that IFN-γ was the only one of cytokines capable of significantly activating the peritoneal macrophages from Toxoplasma-infected mice.

Figures


	Fig. 1 Production of hydrogen peroxide by cytokine-treated macrophages from normal and Toxoplasma-infected mice. The peritoneal macrophages were incubated 24 hours after treatment of cytokine. All results are mean ± standard error of 3 experiments. ^*Indicates values significantly different (P<0.05) from those for resident macrophages.


	Fig. 2 Production of nitrite by cytokine-treated macrophages from normal and Toxoplasma-infected mice.


	Fig. 3 Anti-Toxoplasma activities of cytokine-treated macrophages from normal and Toxoplasma-infected mice.

Tables


	Table 1 Correlation coefficient form the production of hydrogen peroxide, nitrite, and anti-Toxoplasma activities of macrophages from normal and Toxoplasma-infected mice

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