Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81
Warning: fopen(upload/ip_log/ip_log_2024-11.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83
Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84 Transmission electron microscopic ultrastructure of the tegument of Fibricola seoulensis
Transmission electron microscopic ultrastructure of the tegument of Fibricola seoulensis
W M Sohn,*1 and S H Lee2
1Department of Parasitology, College of Medicine, Inje University, Pusan 614-735, Korea.
2Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea.
Received September 25, 1993; Accepted October 19, 1993.
Abstract
An electron microscopic study was performed to observe the ultrastructure of the tegument of F. seoulensis. The outer surface of the tegument was covered with a trilaminated plasma membrane. The electron-dense cytoplasmic layer was 2.5 µm wide in the anterior portion and contained numerous vacuoles, mitochondria and granular materials in its matrix. The basement layer was 330 nm wide or so, and its numerous extensions protruded into the cytoplasmic layer. The sensory organ was composed of a small vesicle of 1.7 × 1.1 µm in dimensions, which possessed a cilium of 1.2 × 0.19 µm in size. The pharynx was composed of the epithelial layer of about 0.5 µm wide, well developed muscle layer and basement layer. The tegument of the oral sucker was composed of a cytoplasmic layer of 0.4-0.5 µm width, a narrow basement layer, a well developed muscle layer and tegumental cells. Some kinds of secretory granules that seemed to be originated from the cells of the oral sucker were observed in the parenchymal portions of the adjacent cells. The tribocytic organ consisted of numerous microvilli. The microvilli were 5 nm wide and heptalaminated. Two types of secretory granules originated from the gland cells of tribocytic organ were observed in the tegument and parenchyme. The tegumental cells were irregular in shape, and of which nuclei were multifarious.
Figures
Figs. 1-3 Fig. 1. The tegument of a metacercaria showing spines (S), nasement layer (BL), circular (CM) and longitudinal muscle bundles (LM), cytoplasmic tbule (CT), and a nucleus (N) in a tegumental cell. × 10,000. Fig. 2. A ciliated sensory organ in the cytoplasmic layer of a 4-day old worm, which consist of a vesicle bearing a cilium, 4-6 mitochondriae (M), numerous vacuoles, granular material (in circle) and septate desmosome (arrow heads). × 4,000. Fig. 3. The tegument on the anterior body of a 4-day old worm showing spins (S), sensory vesicle (arrow mark), basement layer (BL), processes of basement layer (arrow heads) and muscle layers. × 0,000.
Figs. 4-6 Fig. 4. The diagrammatic representation of Fig. 3. × 24,000. Fig. 5. The tegument on the posterior body of a 9-day old worm. × 10,000. Fig. 6. The tegument on the out-surface of the tribocytic organ of a 9-day old worm, showing the spine (S), extensions of basement layer (arrow heads), circular (CM) and longitudinal muscle layer (LM). × 15,000.
Figs. 7-10 Fig. 7. The tegument of the pharynx showing epitherial layer (EL), pharyngeal lumen (PL) and well developed muscle bundles. × 10,000. Fig. 8. The opposite protion of the pharyngeal lumen. Note the basement layer with highly laminated extensions (arrow heads). × 15,000. Fig. 9. A portion of oral sucker in a metacercaria, which composed of a cytoplasmic layer (CL), a narrow basement layer, well developed muscle layer and tegumental cells with a electron-dense nucleus (N). × 10,000. Fig. 10. A protion of oral sucker of a 9-day old worm showing well developed and compacted muscle fibers. × 10,000.
Figs. 11-14 Fig. 11. The electron-dense secretory granules in the parenchymal portion of a metacercaria, which seemed to be originated from the cells in the oral sucker. × 5,000. Fig. 12. Two type of secretory granules in the shoulder region of the oral sucker. × 11,250. Fig. 13. The electron-lucent secretory granules in the cytoplasmic portion of the oral sucker. × 15,000. Fig. 14. A part of ventral sucker showing well developed muscle bundles and tegumental cell, which contained numerous mitochondriae and polysomes. × 11,250.
Figs. 15-16 Fig. 15. The transverse sectional view of the chambered microvillous structure in the tribocytic organ. × 15,000. Fig. 16. The magnification of a portion in Fig. 15. Note the heptalaminated structure. × 41,000.
Figs. 17-18 Fig. 17. The diagrammatic representation of the tribocytic organ. Note the muscle layer (ML), basement layer (BL), cytoplasmic layer (CL) and microwillous structures. × 15,000. Fig. 18. The diagrammatic representation of the tribocytic organ, showing the chambered microvilli, numerous secretory granules and relatively large-sized mitochondriae. × 12,000.
Figs. 19-20 Fig. 19. The tegumental cells in the parenchyme of a 9-day old worm, showing the chromatin patches in the oval-shaped nucleus (N) and numerous mitochondriae (M) in the cytoplasm. × 10,000. Fig. 20. The tegumental cell in oral sucker of a 4-day old worm showing the eccentric nucleolus in the round-shaped nucleus (N) and numerous mitochondriae (M) and ribosomes in the cytoplasm. × 12,000.
References
4.
Bjoerkman N, Thorsell W. On the Fine Structure and Resorptive Function of the Cuticle of the Liver Fluke, Fasciola Hepatical. Exp Cell Res 1964;33:319–329.
5.
Dixon KE, Mercer EH. The fine structure of the nervous system of the cercaria of the liver fluke, Fasciola hepatica L. J Parasitol 1965;51(6):967–976.
6.
Erasmus DA. The host-parasite interface of Cyathocotyle bushiensis Khan, 1962 (Trematoda: Strigeoidea)l. II. Electron microscope studies of the tegument. J Parasitol 1967;53(4):703–714.
7.
Erasmus DA. Studies on the host-parasite interface of strigeoid trematodes. IV. The ultrastructure of the lappets of Apatemon gracilis minor Yamaguti, 1933. Parasitology 1969;59(1):193–201.
8.
Erasmus DA. Studies on the host-parasite interface of strigeoid trematodes. V. Regional differentiation of the adhesive organ of Apatemon gracilis minor Yamaguti, 1933. Parasitology 1969;59(1):245–256.
9.
Erasmus DA. Studies on the host-parasite interface of strigeoid trematodes. VI. Ultrastructural observations on the lappets of Diplostomum phoxini Faust, 1918. Z Parasitenkd 1969;32(1):48–58.
10.
Erasmus DA. Z Parasitenkd 1970;33:211–224.
11.
Hockley DJ. J Parasitol 1970;56:151.
12.
Hockley DJ, McLaren DJ. Schistosoma mansoni: changes in the outer membrane of the tegument during development from cercaria to adult worm. Int J Parasitol 1973;3(1):13–25.
13.
Hong SJ, Lee SH, Seo BS, Hong ST, Chai JY. Studies On Intestinal Trematodes In Korea: IX. Recovery Rate And Development Of Fibricola Seoulensis In Experimental Animals. Korean J Parasitol 1983;21(2):224–233.
14.
Hong ST, Chai JY, Lee SH. Ten human cases of Fibricola seoulensis infection and mixed one with Stellantchasmus and Metagonimus. Korean J Parasitol 1986;24(1):95–97.
15.
Hong ST, Cho TK, Hong SJ, Chai JY, Lee SH, Seo BS. Fifteen human cases of Fibricola seoulensis infection in Korea. Korean J Parasitol 1984;22(1):61–65.
16.
Lee DL. The structure of the helminth cuticle. Adv Parasitol 1972;10:347–379.
17.
Lee SH, et al. Seoul J Med 1985;26(1):52–63.
18.
Lumsden RD. Surface ultrastructure and cytochemistry of parasitic helminths. Exp Parasitol 1975;37(2):267–339.
19.
Lyons KM. Parasitology 1969;59:611–623.
20.
Morris GP, Threadgold LT. A presumed sensory structure associated with the tegument of Schistosoma mansoni. J Parasitol 1967;53(3):537–539.
21.
Ohman C. Parasitology 1965;55:481–502.
22.
Ohman C. The structure and function of the adhesive organ in strigeid trematodes. 3. Apatemon gracilis minor Yamaguti, 1933. Parasitology 1966;56(2):209–226.
23.
Ohman C. The structure and function of the adhesive organ in strigeid trematodes. Parasitology 1966;56(3):481–491.
24.
Seo BS. Seoul J Med 1990;31(2):61–96.
25.
Seo BS, Rim HJ, Lee CW. Studies on the parasitic helmiths of Korea: I. Trematodes of rodents. Korean J Parasitol 1964;2(1):20–26.
26.
Seo BS, Cho SY, Hong ST, Hong SJ, Lee SH. Studies On Parasitic Helminths Of Korea 5.Survey On Intestinal Trematodes Of House Rats. Korean J Parasitol 1981;19(2):131–136.
27.
Seo BS, Lee SH, Hong ST, Hong SJ, Kim CY, Lee HY. Studies On Intestinal Trematodes In Korea: V. A Human Case Infected By Fibricola Seoulensis (Trematoda: Diplostomatidae). Korean J Parasitol 1982;20(2):93–99.
28.
Smith JH, Reynolds ES, Von Lichtenberg F. The integument of Schistosoma mansoni. Am J Trop Med Hyg 1969;18(1):28–49.
PDF Links
Full text via DOI
Download Citation 
