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Interleukin-2 production and alteration of T cell subsets in mice infected with Naegleria fowleri
C J Lyu,C O Shin and K Im*
Department of Parasitology & Institute of Tropical Medicine, College of Medicine, Yonsei University, Seoul 120-752, Korea.
Received July 02, 1993; Accepted July 31, 1993.
Abstract
Naegleria fowleri is the cause of primary amoebic meningoencephalitis in man. IL-2 levels after stimulation of T lymphocytes by PHA or N. fowleri lysates, the amounts of T lymphocyte subsets and the blastogenic responses of T lymphocytes in mice after infected with pathogenic N. fowleri were studied comparing between two study groups, one 1 × 104 trophozoites inoculated mice and the other 1 × 105 trophozoites inoculated mice. All experimental samples were obtained on the day 7, 14 and 24 after inoculation. The mice inoculated with 1 × 104 trophozoites showed a 14.3% mortality rate, and 72.2% in the mice inoculated with 1 × 105 trophozoites. The IL-2 levels on day 14 of two experimental groups were significantly decreased as compared with the control group. Thy 1.2+ T cells in the total spleen lymphocytes of 1 × 105 trophozoites inoculated group on day 7 were significantly increased compared with the control group. There was no significant difference between 1 × 104 trophozoites inoculated group and the control group. L3T4+ T cells and Ly2+ T cells in the total spleen lymphocytes of 1 × 105 trophozoites inoculated group on day 7 were significantly increased compared with the control group. The DNA S fraction of T cells in the spleen of 1 × 105 trophozoites inoculated group was significantly increased on day 7. The amount of S fractions of DNA were sequentially decreased on day 14 and 24, but they were also significantly increased compared with the control group.(ABSTRACT TRUNCATED AT 250 WORDS)
Figures
Fig. 1 Mortality of mice inoculated with N. fowleri trophozoites.
Fig. 2 The ratio of Thy 1,2+ T cells to spleen lymphocytes in mice inoculated with N. fowleri trophozoites.
Fig. 3 The ratio of L3T4+ T cells to spleen lymphocytes in mice inoculated with N. fowleri trophozoites.
Fig. 4 The ratio of Ly2+ T cells to spleen lymphocytes in mice inoculated with N. fowleri trophozoites.
Fig. 5 The ratio of L3T4+ T/Ly2+ T cells to spleen lymphocytes in mice inoculated with N. fowleri trophozoites.
Tables
Table 1 IL-2 production of spleen cells treated with PHA, N. fowleri lysates in mice inoculated with N. fowleri trophozoites
Table 2 S phase fraction of splenic T cells in mice inoculated with N. fowleri trophozoites
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