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Antigen specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid in tissue invading nematodiasis
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Korean J Parasito > Volume 31(2):1993 > Article

Brief Communication
Korean J Parasitol. 1993 Jun;31(2):169-171. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1993.31.2.169
Copyright © 1993 by The Korean Society for Parasitology
Antigen specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid in tissue invading nematodiasis
N Morakote,2 and Y Kong*2
1Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50002, Thailand.
Received February 23, 1993; Accepted March 29, 1993.

Abstract

Diagnostic specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid (sparganum) was evaluated by micro-ELISA in tissue invading nematodiasis such as 25 gnathostomiasis, 33 angiostrongyliasis, 22 trichinellosis patients, and 20 normal control. All but one patient each in 3 nematodiases showed the antibody levels of negative range. The positively reacted patients were regarded as concomitant infections of sparganum because immunized or hyperinfected rabbit serum of the nematodes did not react crossly to the antigen.

Tables


Table 1
Mean (M) and standard deviation (S.D.) of abs. for sparganum-specific (IgG) antibody by micro-ELISA in gnathostomiais, angiostrongyliasis, trichinellosis and normal control against 36 and 31 kDa proteins of sparganum


Table 2
Antibody levels (ads.) in immunized or hyperinfected rabbit serum against each antigen

References
1. Cho SY, Kang SY, Kong Y. Purification of antigenic protein of sparganum by immunoaffinity chromatography using a monoclonal antibody. Korean J Parasitol 1990;28(3):135–142.
 
2. Chotimongkol V. Srinagarind Hosp Med 1980;2:251–256.
4. Kong Y, Kang SY, Cho SY. Single step purification of potent antigenic protein from sparganum by gelatin-affinity chromatography. Korean J Parasitol 1991;29(1):1–7.
 
5. Morakote N, et al. J Parasit Trop Med Ass Thailand 1987;10:43–50.
6. Morakote N, et al. J Parasit Trop Med Ass Thailand 1991;14:9–13.
7. Morakote N, Khamboonruang C, Siriprasert V, Suphawitayanukul S, Marcanantachoti S, Thamasonthi W. The value of enzyme-linked immunosorbent assay (ELISA) for diagnosis of human trichinosis. Trop Med Parasitol 1991;42(3):172–174.
 
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