Applicability of ABC-ELISA and protein A-ELISA in serological diagnosis of cysticercosis

, Lee, J H; , Kong, Y; , Ryu, J Y; , Cho, S Y

doi:1993.31.1.49

Korean J Parasito > Volume 31(1):1993 > Article

Original Article


Korean J Parasitol. 1993 Mar;31(1):49-56. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1993.31.1.49
Copyright © 1993 by The Korean Society for Parasitology


Applicability of ABC-ELISA and protein A-ELISA in serological diagnosis of cysticercosis
Jong Hyun Lee,Yoon Kong,Jae-Young Ryu and Seung-Yull Cho^*
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.

Received December 19, 1992; Accepted January 26, 1993.
Abstract
Specific antibody test in serum and cerebrospinal fluid (CSF) is still the main mode of serological diagnosis of cysticercosis. Of different techniques of antibody test, enzyme-linked immunosorbent assay (micro-ELISA) has widely been applied. This study was undertaken to observe whether diagnostic capability can be improved by applying more sensitive techniques such as Protein A-ELISA and avidin biotin complex ELISA (ABC-ELISA). When evaluated using 115 sera of human cysticercosis, the antibody positive rates were not significantly improved in Protein A-ELISA (82.6%) and in ABC-ELISA (86.1%) than in micro-ELISA (81.7%). The specificities, evaluated in 165 sera from other diseases and normal controls, were significantly improved (88.5% by micro-ELISA, 93.3% by Protein A-ELISA and 93.8% by ABC-ELISA). Antibody levels (absorbance, abs.) in individual serum were correlated well (r = 0.83-0.86) each other. An actual benefit of Protein A-ELISA and ABC-ELISA was that they needed smaller amount of test sample.

Figures


	Fig. 1 Relations of the individual antibody levels (abs.) as measured by micro-ELISA and Protein A-ELISA in sera of 115 cysticercosis patients. Horizontal and vertical dotted lines of abs. 0.18 or 0.10 are the positive criteria.


	Fig. 2 Relations of the individual antibody levels (abs.) as measured by micro-ELISA and ABC-ELISA in sera of 115 cysticercosis patients. Dotted lines at abs. 0.18 and 0.10 are the same as described in Fig. 1.


	Fig. 3 Distribution and relation of the specific antibody levels (abs.) between micro-ELISA and Protein A-ELISA to T. solium metacestode antigen in 165 sera of other diseases and normal controls. Horizontal and vertical lines of abs. 0.18 and 0.10 are the positive criteria.


	Fig. 4 Distribution and relation of the specific antibody levels (abs.) between micro-ELISA and ABC-ELISA to T. solium metacestode antigen in 165 sera of other diseases and normal controls. Lines and symbols are the same as described in Fig. 3.

Tables


	Table 1 Procedures and conditions of 3 different ELISA techniques employed in this study


	Table 2 Results of specific-antibody tests by 3 different techniques of ELISA in each category of patients


	Table 3 Sensitivity and specificity of three techqniques of ELISA as calculated in 115 cysticercosis and 165 other diseases and normal control

References


1.	Baily GG, Mason PR, Trijssenar FE, Lyons NF. Serological diagnosis of neurocysticercosis: evaluation of ELISA tests using cyst fluid and other components of Taenia solium cysticerci as antigens. Trans R Soc Trop Med Hyg 1988;82(2):295–299.

2.	Bayer EA, Wilchek M. The use of the avidin-biotin complex as a tool in molecular biology. Methods Biochem Anal 1980;26:1–45.

3.	Chang KH, Kim WS, Cho SY, Han MC, Kim CW. Comparative evaluation of brain CT and ELISA in the diagnosis of neurocysticercosis. AJNR Am J Neuroradiol 1988;9(1):125–130.

4.	Cho SY, Hong ST, Rho YH, Choi SY, Han YC. Application of micro-ELISA in serodiagnosis of Human paragonimiasis. Korean J Parasitol 1981;19(2):151–156.

5.	Cho SY, Kim SI, Kang SY, Choi DY, Suk JS, Choi KS, Ha YS, Chung CS, Myung HJ. Evaluation of enzyme-linked immunosorbent assay in serological diagnosis of human neurocysticercosis using paired samples of serum and cerebrospinal fluid. Korean J Parasitol 1986;24(1):25–41.

6.	Choi BK, et al. Chung-Ang J Med 1986;11:135–146.

7.	Flisser A, Woodhouse E, Larralde C. Human cysticercosis: antigens, antibodies and non-responders. Clin Exp Immunol 1980;39(1):27–37.

9.	Goding JW. Use of staphylococcal protein A as an immunological reagent. J Immunol Methods 1978;20:241–253.

*10.*	Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem 1979;27(8):1131–1139.

*11.*	Kendall C, Ionescu-Matiu I, Dreesman GR. Utilization of the biotin/avidin system to amplify the sensitivity of the enzyme-linked immunosorbent assay (ELISA). J Immunol Methods 1983;56(3):329–339.

*12.*	Kim H, Kim SI, Cho SY. Serological Diagnosis Of Human Sparganosis By Means Of Micro-ELISA. Korean J Parasitol 1984;22(2):222–228.

*13.*	Kronvall G. A surface component in group A, C, and G streptococci with non-immune reactivity for immunoglobulin G. J Immunol 1973;111(5):1401–1406.

*14.*	Larralde C, Laclette JP, Owen CS, Madrazo I, Sandoval M, Bojalil R, Sciutto E, Contreras L, Arzate J, Diaz ML, et al. Reliable serology of Taenia solium cysticercosis with antigens from cyst vesicular fluid: ELISA and hemagglutination tests. Am J Trop Med Hyg 1986;35(5):965–973.

*15.*	Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265–275.

*16.*	McLaren M, Draper CC, Roberts JM, Minter-Goedbloed E, Ligthart GS, Teesdale CH, Amin MA, Omer AH, Bartlett A, Voller A. Studies on the enzyme linked immunosorbent assay (ELISA) test for Schistosoma mansoni infections. Ann Trop Med Parasitol 1978;72(3):243–253.

*17.*	Nash TE, Neva FA. Recent advances in the diagnosis and treatment of cerebral cysticercosis. N Engl J Med 1984;311(23):1492–1496.

*18.*	Reed SG, Shreffler WG, Burns JM Jr, Scott JM, Orge Mda G, Ghalib HW, Siddig M, Badaro R. An improved serodiagnostic procedure for visceral leishmaniasis. Am J Trop Med Hyg 1990;43(6):632–639.

*19.*	Shim YS, et al. Seminars Resp Med 1991;12:35–45.

*20.*	Bartlett A, Bidwell DE. Enzyme immunoassays for parasitic diseases. Trans R Soc Trop Med Hyg 1976;70(2):98–106.