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Antigenic localities in the tissues of Metagonimus yokogawai in the period of growth
H J Rim,S J Kim,* and M G Yang
Department of Parasitology and Institute for Tropical Endemic Diseases, College of Medicine, Korea University, Seoul 136-705, Korea.
Abstract
In order to observe the antigenic localization in the tissues of Metagonimus yokogawai in growth stages, immunogoldlabeling method was applied to using serum of the cat which infected with isolated metacercariae from Plecoglossus altivelis. The sectioned worm tissues from each growth stages were embedded in Lowicryl HM 20 medium, stained with infected serum IgG and protein A gold complex (particle size: 12 nm) and observed by electron microscopy. In the worm tissues of all experimental groups, the gold particles were specifically concentrated on the tegumental syncytium and cytoplasm of the tegumental cell as well as the secretory granules in the parenchymal tissue. In the 16th and 20th week grown worm tissues, the gold particles were specifically concentrated on the vesicles in the tegumental syncytium and cytoplasm of the tegumental cell. The gold particles were specifically concentrated on the caecal epithelia of the 4th, 8th and 12th week growth groups but slightly concentrated on those of the 16th and 20th week.
Figures
Figs. 1-4 Figs. 1~5. Electron micrographs of the tegument of Metagonimus yokogawai.
Fig. 1. The teguments of all growth stage worms which were reacted with control cat sera showed the tegumental syncytium(TS), basal layer(BL), circular muscle(CM) layer, intersitial matrix(IM) and tegumental cell cytoplasm(TCC), The gold particle was not labeled on the tegument or other portions of the tissue. Bar = 1 µm(×20,000)
Fig. 2. The tegument of the worms at 16 and 20 weeks grown which was reacted with sera from cats after infection. The gold particle was not labled on the tegumental syncytium but many vesicle (V) observed on the tegumental syncytium and tegumental cell cytoplasm. Bar = 1 µm(×20,000)
Fig. 3. The tegument of the worms at 16 and 20 weeks grown which was reacted with sera from cats at all infected groups. Gold particles specifically labeled the vesicle in the tegumental syncytium (TS).
Fig. 4. The tegument cell of the worms at 16 and 20 weeks grown which was reacted with sera from cats at all infected groups. Gold particle was predominately labeled on the vesicle of the tegumental syncytium (TS). Bar = 1 µm(×20,000)
Figs. 5-8 Figs. 1~5. Electron micrographs of the tegument of Metagonimus yokogawai.
Fig. 5, 6 & 7. The geguments of the worms at 4,8 and 12 weeks grown which reacted with sera from cats at all infected groups, Gold particles were labeled on the tegumental syncytium(TS). Bar = 1 µm(×20,000)
Figs. 8~11. Electron micrographs of the epithelial lamellae and lumen area of the caeca of worms.
Fig. 8. The caeca of the worm at 4 weeks growth stage which reacted with sera from cats at all infected groups showed well developed lamellae and lumen. Gold particles were specifically labeled the lamellae and lumen matrix. Bar = 1 µm(×20,000)
Figs. 9-12 Fig. 9. The caeca of the worm at 8 weeks growth stage which reacted with sera from cats at all infected groups. Gold particles were specifically labeled on the lamellae and lumen matrix. Bar = 1 µm(×20,000)
Fig. 10. The caeca of the worm at 12 weeks growth stage which reacted with sera from cats at all infected groups. Gold particles were specifically labeled on the lamellae and lumen matrix. Bar = 1 µm(×20,000)
Fig. 11. The caeca of the worm at 16 and 20 weeks growth stage which reacted with sera from cats at 16 and 20 weeks after infection. Gold particles were specifically labeled on the lamellae and lumen matrix. Bar = 1 µm(×20,000)
Fig. 12 & 13. Electron micrographs of the secretory granules in the parenchymal cell at the all growth stages of the worm.
Fig. 12. The parenchymal cell of the worm which reacted with sera from cats at 4 weeks after infection showed well-developed rough endoplasmic reticulum(RER) in the cytoplasm. Gold particles were slightly labeled on the secretory granules(SG). Bar = 1 µm(×20,000)
Figs. 13-15 Fig. 12 & 13. Electron micrographs of the secretory granules in the parenchymal cell at the all growth stages of the worm.
Fig. 13. The parenchymal cell of the worm which reacted with sera from cats at 16 weeks after infection. Gold particles were specifically labeled on the secretory granules. Bar = 1 µm(×20,000)
Fig. 14. Electron micrograph of the granules in the vietlline gland of the worm at all growth stage which reacted with sera from cats at all infected groups. Gold particles were slightly labeled on granules (G). Bar = 1 µm(×20,000)
Fig. 15. Electron micrograph of the excretory bladder of the worm at all growth stages, the excretory bladder of the worm which reacted with sera from cats at all infected groups. Gold particles were predominantly labeled on the epithelial lamella area in the excretory bladder. Bar = 1 µm(×20,000)
Tables
Table 1 Quantitative density of the labeled gold particles in each tissues of Metagonimus yokogawai reacted with antibodyes (IgG)* obtained from cats infected with M. yokogawai. (Mean No. of gold particles/0.1 µm2 of each tissue of the worm of age)
Table 2 Quantitative density of the labeled gold particles in each tissues of Metagonimus yokogawai reacted with antibodyes (IgG)* obtained from cats infected with M. yokogawai. (Mean No. of gold particles/0.1 µm2 of each tissue of the worm of age)
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