Detection of antibodies in serum and cerebrospinal fluid to <i>Toxoplasma gondii</i> by indirect latex agglutination test and enzyme-linked immunosorbent assay

, Choi, W Y; , Nam, H W; , Youn, J H; , Kim, D J; , Kong, Y; , Kang, S Y; , Cho, S Y

doi:1992.30.2.83

Korean J Parasito > Volume 30(2):1992 > Article

Original Article


Korean J Parasitol. 1992 Jun;30(2):83-90. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1992.30.2.83
Copyright © 1992 by The Korean Society for Parasitology


Detection of antibodies in serum and cerebrospinal fluid to Toxoplasma gondii by indirect latex agglutination test and enzyme-linked immunosorbent assay
W Y Choi,H W Nam,J H Youn,D J Kim,Y Kong,^S Y Kang,^ and S Y Cho^**
Department of Parasitology, Catholic University Medical College, Seoul 137-701, Korea.


Abstract
Sensitivity of anti-Toxoplasma antibody (IgG) test by enzyme-linked immunosorbent assay (ELISA) was evaluated in comparison with indirect latex agglutination (ILA) using 2,016 paired human samples of serum and cerebrospinal fluid (CSF). The samples were collected from neurologic patients in Korea with mass lesions in central nervous system (CNS) as revealed by imaging diagnosis (CT/MRI). When the sera were screened for anti-Toxoplasma antibody by ILA, 76 cases(3.8%) were positive (1:32 or higher titers). In the paired samples of CSF, no positive reactions were observed. When ELISA was performed using PBS extract of Percoll purified tachyzoites as antigen, cut-off absorbance was determined as 0.40 for serum and 0.27 for CSF tests. The antibody positive rates by ELISA were 7.0% in serum and 5.6% in CSF. Of them, 40 cases (2.0%) showed positive reactions in both serum and CSF. The antibody positive rates were higher in groups older than 40 years. The rates were higher in male (4.7% by ILA, 8.3% by ELISA) than in female (2.2% by ILA, 5.0% by ELISA). The rates in CSF showed no such sex difference. ELISA showed twice higher positive rates when serum was tested, and was sensitive enough to detect specific antibodies in CSF. Etiologic relations between positive antibody tests and CNS lesions remained unknown.

Figures


	Fig. 1 Relations between anti-Toxoplasma antibody (IgG) in sera by ELISA and the ILA titers.


	Fig. 2 Relations between anti-Toxoplasma antibody (IgG) in CSF by ELISA and ILA titers.


	Fig. 3 Comparison of percent of positive between ILA and ELISA. ▪, serum and □, CSF.


	Fig. 4 Correlationship between ELISA values of the sera and CSF among ILA positive cases. ○, 1:32; •, 1:64; □, 1:128; ▪, 1:256; ▵, 1:512 and ▴, 1:1,024 or higher.


	Fig. 5 Profile of antibody positive rates by age. ○, sera by ILA; •, sera by ILISA and ▿, CSF by ELISA


	Fig. 6 Positive rates of anti-Toxoplasma antibodies by sex. ▪, male and □, female.

Tables


	Table 1 Frequencies of serological ILA titers over 1:8


	Table 2 ILA titers of CSF of which the serum titers are 1:1,024 or higher


	Table 3 Prevalence of ElISA positive cases by ILA titers in 2,016 neurologic patients


	Table 4 Screening of sera by ILA for anti-Toxoplasma antibody


	Table 5 Screening of sera and CSF for anti-Toxoplasma antibodies ELISA

References


1.	Cho SY, Kim SI, Kang SY, Choi DY, Suk JS, Choi KS, Ha YS, Chung CS, Myung HJ. Evaluation of enzyme-linked immunosorbent assay in serological diagnosis of human neurocysticercosis using paired samples of serum and cerebrospinal fluid. Korean J Parasitol 1986;24(1):25–41.

2.	Cho SY, Kim SI, Kang SY, Park AJ. Intracranial synthesis of specific IgG antibody in cerebrospinal fluid of neurocysticercosis patients. Korean J Parasitol 1988;26(1):15–26.

3.	Choi WY. J Catholic Med Coll 1959;16:229–253.

4.	Choi WY, Yoo JU, Kim WG. [Toxoplasma antibodies by indirect latex agglutination tests in St. Mary's Hospital patients]. Korean J Parasitol 1982;20(1):33–37.

5.	Choi WY, Yoo JE, Chung CS, Paik KK, Cho SN. [Toxoplasma Antibodies By Indirect Latex Agglutination Tests In National Seoul Mental Hospital Patients]. Korean J Parasitol 1983;21(2):281–285.

6.	Choi WY, et al. Korean Cent J Med 1984;46:419–423.

7.	Choi WY, Choi HR, Rha JG. [Significance of Toxoplasma antibody titers by indirect latex agglutination tests in pregnant women and pelvic tumor patients]. Korean J Parasitol 1985;23(2):300–304.

8.	Choi WY, Yoo JE, Nam HW, Oh CY, Kim SW, Katakura K, Kobayashi A. Toxoplasma antibodies by indirect latex agglutination tests in zoo animals. Korean J Parasitol 1987;25(1):13–23.

9.	Choi WY, Nam HW, Youn JH, Kim WS, Kim WK. Toxoplasma antibody titers by indirect latex agglutination test in patients of Kangnam St. Mary's Hospital and Cheju Medical Center. Korean J Parasitol 1989;27(3):171–175.

*10.*	Kasper LH, Bradley MS, Pfefferkorn ER. Identification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies. J Immunol 1984;132(1):443–449.

*11.*	Kim TJ, et al. J Catholic Med Coll 1983;36:133–137.

*12.*	Kobayashi A, et al. Jpn J Parasitol 1977;26:175–180.

*13.*	Levy RM, Pons VG, Rosenblum ML. Central nervous system mass lesions in the acquired immunodeficiency syndrome (AIDS). J Neurosurg 1984;61(1):9–16.

*14.*	Lunde MN, Jacobs L. Antigenic differences between endozoites and cystozoites of Toxoplasma gondii. J Parasitol 1983;69(5):806–808.

*15.*	Makioka A, Suzuki Y, Kobayashi A. Recognition of tachyzoite and bradyzoite antigens of Toxoplasma gondii by infected hosts. Infect Immun 1991;59(8):2763–2766.

*16.*	McLaren M, Draper CC, Roberts JM, Minter-Goedbloed E, Ligthart GS, Teesdale CH, Amin MA, Omer AH, Bartlett A, Voller A. Studies on the enzyme linked immunosorbent assay (ELISA) test for Schistosoma mansoni infections. Ann Trop Med Parasitol 1978;72(3):243–253.

*17.*	Moon JB, et al. Office of Rural Development 1965;8:143.

*18.*	Sabin AB, Feldman HA. Dyes as Microchemical Indicators of a New Immunity Phenomenon Affecting a Protozoon Parasite (Toxoplasma). Science 1948;108(2815):660–663.

*19.*	Soh CT, et al. Yonsei Med J 1960;1:52–54.

*20.*	Tsubota N, et al. Jpn J Parasitol 1977;26:286–290.