Blastogenesis of splenic lymphocytes to specific antigens and PHA in <i>Paragonimus westermani</i> infected mice

, Min, D Y; , Shin, M H; , Choi, R

doi:1992.30.1.43

Korean J Parasito > Volume 30(1):1992 > Article

Original Article


Korean J Parasitol. 1992 Mar;30(1):43-48. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1992.30.1.43
Copyright © 1992 by The Korean Society for Parasitology


Blastogenesis of splenic lymphocytes to specific antigens and PHA in Paragonimus westermani infected mice
D Y Min,M H Shin and R Choi
Department of Parasitology, College of Medicine, Hanyang University, Seoul 133-791, Korea.


Abstract
Paragonimus westermani is a common fluke in Korea. The present study aimed to observe the cell mediated immune response in experimental paragonimiasis of mice. The mouse (BALB/c) was orally inoculated with 40 metacercariae of P. westermani from Cambaroides similis. During the infection (1, 2, 4, 6 weeks) of mouse, blastogenic response of splenic lymphocytes to P. westermani adult antigen, metacercaria antigen, and PHA were observed. Sera from infected and noninfected mice added to normal mouse splenic lymphocytes with or without PHA. The blastogenic response of splenic lymphocytes to PHA was reduced after 1 week of infection. However after 6 weeks of infection, the response was restored to the control level. The blastogenic response of splenic lymphocytes to P. westermani adult or metacercaria antigen increased significantly on 1 week after infection, and maintained up to 6 weeks after infection. The response of non-infected mice was suppressed by addition of the infected mouse serum. The present results suggested that cellular immunity was involved in P. westermani infected mice and that P. westermani anti-serum inhibited proliferation of T lymphocytes.

Figures


	Fig. 1 Enhancement rate of mouse splenocytes after treatment with mitogens (PHA: phytohemagglutinin, MA: metacercarial antigen, AWA: adult worm antigen).

Tables


	Table 1 Stimulation index of mouse splenic lymphocytes after treatment with mitogens


	Table 2 Suppression rate of noninfected mouse splenic lymphocytes after addition of infected mouse sera

References


1.	Ahn MH, Colley DG. [Immunological Responses By Soluble Egg Antigen Of Schistosoma Mansoni In Mice]. Korean J Parasitol 1984;22(2):203–208.

2.	Baird LG, Kaplan AM. Macrophage regulation of mitogen-induced blastogenesis. I. Demonstration of inhibitory cells in the spleens and peritoneal exudates of mice. Cell Immunol 1977;28(1):22–35.

3.	Bout D, et al. Ann Immunol 1977;128(c):687–698.

4.	Carvalho EM, Johnson WD, Barreto E, Marsden PD, Costa JL, Reed S, Rocha H. Cell mediated immunity in American cutaneous and mucosal leishmaniasis. J Immunol 1985;135(6):4144–4148.

5.	Colley DG. Schistosomal egg antigen-induced lymphocyte blastogenesis in experimental murine Schistosoma mansoni infection. J Immunol 1971;107(5):1477–1480.

6.	Cottrell BJ, Humber D, Sturrock RF. An immunosuppressive factor in the serum of patients with schistosomiasis. Trans R Soc Trop Med Hyg 1980;74(3):415–416.

7.	Cottrell BJ, Sturrock RF, Vanhoegaerden M. An immunosuppressive factor in the serum of baboons (Papio anubis) infected with Schistosoma mansoni. Immunology 1980;39(4):589–598.

8.	Dessaint JP, Camus D, Fischer E, Capron A. Inhibition of lymphocyte proliferation by factor(s) produced by Schistosoma mansoni. Eur J Immunol 1977;7(9):624–629.

9.	Jayawardena AN, Waksman BH. Suppressor cells in experimentally trypanosomiasis. Nature 1977;265(5594):539–541.

*10.*	Kamal KA, Higashi GI. Suppression of mitogen-induced lymphocyte transformation by plasma from patients with hepatosplenic Schistosomiasis mansoni: role of immune complexes. Parasite Immunol 1982;4(4):283–298.

*11.*	Kanazawa T, Hata H, Kojima S, Yokogawa M. Paragonimus westermani: a comparative study on the migration route of the diploid and triploid types in the final hosts. Parasitol Res 1987;73(2):140–145.

*12.*	Lee SH, Sung SH, Chai JY. [Immunohistochemical study on the antigenicity of body compartments of Paragonimus westermani]. Korean J Parasitol 1989;27(2):109–117.

*13.*	Letonja T, Hammerberg C, Schurig G. Evaluation of spleen lymphocyte responsiveness to a T-cell mitogen during early infection with larval Taenia taeniaeformis. Parasitol Res 1987;73(3):265–270.

*14.*	Lightowlers MW, Rickard MD. Excretory-secretory products of helminth parasites: effects on host immune responses. Parasitology 1988;96 Suppl:S123–S166.

*15.*	Min DY, Ahn MH, Kim KM, Leem MH, Park SY. [The effects of antibodies and complement in macrophage-mediated cytotoxicity on metacercariae of the lung fluke, Paragonimus westermani]. Korean J Parasitol 1990;28(2):91–100.

*16.*	Oldham G, Williams L. Cell mediated immunity to liver fluke antigens during experimental Fasciola hepatica infection of cattle. Parasite Immunol 1985;7(5):503–516.

*17.*	Pelley RP, Ruffier JJ, Warren KS. Suppressive effect of a chronic helminth infection, schistosomiasis mansoni, on the in vitro responses of spleen and lymph node cells to the T cell mitogens phytohemagglutinin and concanavalin A. Infect Immun 1976;13(4):1176–1183.

*18.*	Ryan JL, Arbeit RD, Dickler HB, Henkart PA. Inhibition of lymphocyte mitogenesis by immobilized antigen-antibody complexes. J Exp Med 1975;142(4):814–826.

*19.*	Stout RD, Herzenberg LA. The Fc receptor on thymus-derived lymphocytes: II. Mitogen responsiveness of T lymphocytes bearing the Fc receptor. J Exp Med 1975;142(5):1041–1051.

*20.*	Bartlett A, Bidwell DE. Enzyme immunoassays for parasitic diseases. Trans R Soc Trop Med Hyg 1976;70(2):98–106.

*21.*	Wongratanacheewin S, Rattanasiriwilai W, Priwan R, Sirisinha S. Immunodepression in hamsters experimentally infected with Opisthorchis viverrini. J Helminthol 1987;61(2):151–156.

*22.*	Yamashita T, Boros DL. Changing patterns of lymphocyte proliferation, IL-2 production and utilization, and IL-2 receptor expression in mice infected with Schistosoma mansoni. J Immunol 1990;145(2):724–731.

*23.*	Yong TS, Kim TS, Lee JS, Lee OY, Kim DC. Detection of circulating antigens in rats experimentally infected with Paragonimus westermani by ELISA. Korean J Parasitol 1987;25(2):141–148.

*24.*	Zimmerman GL, Kerkvliet NI, Brauner JA, Cerro JE. Modulation of host immune responses by Fasciola hepatica: responses by peripheral lymphocytes to mitogens during liver fluke infections of sheep. J Parasitol 1983;69(3):473–477.