Abstract
An immunohistochemical study was performed to demonstrate comparative antigenicity of each body structure of the liver fluke, Clonorchis sinensis, such as the digestive tract, reproductive organs, excretory system, tegument and suckers.
Indirect immunoperoxidase technique was applied, using formalin-fixed and paraffin-embedded sections of C. sinensis as the antigen. Pooled cat sera obtained 10 weeks after an experimental infection with C. sinensis and peroxidase-conjugated goat anti-cat IgG were used as the primary and secondary antibodies, respectively. The intensity of immunohistochemical stain was very sensitive upon the titers of the primary and secondary antibodies, and their optimum dilutions were found to be 1:1,000-1:2,000 and 1:1,000, respectively. The intestinal epithelial cells, intestinal content and excretory bladder showed strong positive coloring reactions even at lower titer (1:2,000) of the primary antibody, whereas the uterine wall and eggs, vitelline glands, and male reproductive organs showed only weak positive reactions despite an increase in the antibody titer (1:1,000). On the other hand, the suckers, tegument, subtegumental cells and other parenchyma portions did not reveal any positive immunoperoxidase reaction at the same antibody titers. From the above results, it is highly suggested that the most potent antigenicity of C. sinensis occur from their excretory-secretory substances originated from the digestive and excretory organs.
Citations
Citations to this article as recorded by
- 41.5-kDa Cathepsin L protease from Clonorchis sinensis: expression, characterization, and serological reactivity of one excretory–secretory antigen
Yanwen Li, Yan Huang, Xuchu Hu, Xiaoquan Liu, Changling Ma, Junhong Zhao, Zhongdao Wu, Jin Xu, Xinbing Yu
Parasitology Research.2012; 111(2): 673. CrossRef - Functional Genes and Proteins of Clonorchis sinensis
Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
The Korean Journal of Parasitology.2009; 47(Suppl): S59. CrossRef - Molecular cloning and analysis of stage and tissue-specific expression of Cathepsin L-like protease from Clonorchis sinensis
Yanwen Li, Xuchu Hu, Xiaoquan Liu, Jing Xu, Fengyu Hu, Changling Ma, Xinbing Yu
Parasitology Research.2009; 105(2): 447. CrossRef - PURIFICATION AND CHARACTERIZATION OF A 7-KDA PROTEIN FROM CLONORCHIS SINENSIS ADULT WORMS
Hye-Jeong Lee, Chang-Seok Lee, Beom-Su Kim, Kyoung-Hwan Joo, Joon-Sang Lee, Tong-Soo Kim, Hak R. Kim
Journal of Parasitology.2002; 88(3): 499. CrossRef - Antigenic profile and localization of Clonorchis sinensis proteins in the course of infection
Sung-Jong Hong, Tae Yun Kim, Kye-Yong Song, Woon-Mok Sohn, Shin-Yong Kang
The Korean Journal of Parasitology.2001; 39(4): 307. CrossRef - A Clonorchis sinensis-specific antigen that detects active human clonorchiasis
S I Kim
The Korean Journal of Parasitology.1998; 36(1): 37. CrossRef - Immune reactions between excretory-secretory antigens and specific antibodies of Clonorchis sinensis before and after praziquantel treatment in experimentally infected rabbits
S I Kim
The Korean Journal of Parasitology.1994; 32(1): 35. CrossRef
