Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81
Warning: fopen(upload/ip_log/ip_log_2024-11.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83
Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84 Component proteins in cystic fluid of Taenia solium metacestodes collected surgically from neurocysticercosis patients
Component proteins in cystic fluid of Taenia solium metacestodes collected surgically from neurocysticercosis patients
Y Kong,S Y Kang and S Y Cho
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
Abstract
Surgically collected cystic fluid of Taenia solium metacestodes from patients of intracranial cystic lesion were compared in their protein composition with those from naturally infected pigs in Cheju Do, Korea and Ecuador. In non-denaturing discontinuous-polyacrylamide gel electrophoresis (disc-PAGE), no discernible differences were recognized in banding patterns between the cystic fluids from Cheju Do and Ecuador, and between the cystic fluids from pigs and human lesions except wider bands that corresponded to human albumin and γ-globulin (in 4 of 9 patients). In reducing SDS-PAGE, bands in the cystic fluid from Ecuador showed the same banding pattern with that from Cheju Do but two bands of 21 and 17 kDa were stained darker. Cystic fluids from patients revealed the same protein compositions of the major protein bands of 94, 64, 15, 10 and 7 kDa as in the cystic fluid of pig origin, but human albumin (66 kDa), heavy and light chains of gamma globulin (55 and 22.5 kDa) were contaminated in 4 of 9 cystic fluids. Human CSF proteins seem to have been contaminated during cystic fluid collection. In any cystic fluid from patients, the major protein component was 150 kDa which was subdivided into 15, 10 and 7 kDa in reducing SDS-PAGE.
Figures
Fig. 1 Disc-PAGE findings of cystic fluid(CF) from pigs and human, and normal DSF at 7.5% gel concentration. 1: CF from Ecuador, 2: CF from Cheju Do, 3: HSC*-895, 4: HSC-4953, 5: HSC-802, 6: HSC-1692, 7: HSC-1702, 8: HSC-2976, 9: HSC-5718, 10: HSC-2922, 11: HSC-7310, 12: Normal CFS
*Our reference file of Human Serum Collection
Fig. 2 SDS-PAGE findings of CF from pigs and human and normal human CSF. Linear gradient gel of 10-15% was used for protein separation. Mr: Molecular weight in kDa. Lane 1-12: Same as described in Fig. 1. ⇦: Human albumin ←: Heavy chain of γ-globulin ⇐:Light chain of γ-globulin ◁: Unidentified band
Tables
Table 1 Protein content and result of antigenicity of cystic fluid which were collected surgically from human brain lesions
References
1.
Baily GG, Mason PR, Trijssenar FE, Lyons NF. Serological diagnosis of neurocysticercosis: evaluation of ELISA tests using cyst fluid and other components of Taenia solium cysticerci as antigens. Trans R Soc Trop Med Hyg 1988;82(2):295–299.
2.
Cervantes-Vazquez M, Correa D, Merchant M, Hicks JJ, Laclette JP. Respiratory changes associated with the in vitro evagination of Taenia solium cysticerci. J Parasitol 1990;76(1):108–112.
3.
Correa D, Laclette JP, Rodriguez-del-Rosal E, Merchant M, Flisser A. Heterogeneity of Taenia solium cysticerci obtained from different naturally infected pigs. J Parasitol 1987;73(2):443–445.
4.
Cho SY, Kang SY, Kim SI. Analysis of antigen specificity using monoclonal and polyclonal antibodies to Cysticercus cellulosae by enzyme-linked immunoelectrotransfer blot technique. Korean J Parasitol 1987;25(2):159–167.
5.
Cho SY, Kim SI, Kang SY, Choi DY, Suk JS, Choi KS, Ha YS, Chung CS, Myung HJ. Evaluation of enzyme-linked immunosorbent assay in serological diagnosis of human neurocysticercosis using paired samples of serum and cerebrospinal fluid. Korean J Parasitol 1986;24(1):25–41.
6.
Cho SY, Kim SI, Kang SY, Kong Y. Biochemical properties of a purified protein in cystic fluid of Taenia solium metacestodes. Korean J Parasitol 1988;26(2):87–94.
7.
Choi BK, et al. Chun-Ang J Med 1986;11:135–146.
8.
Davis BJ. Disc Electrophoresis. II. Method and Application to Human Serum Proteins. Ann N Y Acad Sci 1964;121:404–427.
9.
Grogl M, Estrada JJ, MacDonald G, Kuhn RE. Antigen-antibody analyses in neurocysticercosis. J Parasitol 1985;71(4):433–442.
10.
Jang JS, et al. J Korean Neurosurg Soc 1985;15:841–847.
11.
Joo KH, et al. Korea Univ Med J 1987;24:139–150.
12.
Kong Y, Kang SY, Cho SY, Min DY. Cross-reacting and specific antigenic components in cystic fluid from metacestodes of Echinococcus granulosus and Taenia solium. Korean J Parasitol 1989;27(2):131–139.
13.
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227(5259):680–685.
14.
Larralde C, Laclette JP, Owen CS, Madrazo I, Sandoval M, Bojalil R, Sciutto E, Contreras L, Arzate J, Diaz ML, et al. Reliable serology of Taenia solium cysticercosis with antigens from cyst vesicular fluid: ELISA and hemagglutination tests. Am J Trop Med Hyg 1986;35(5):965–973.
15.
Lowry OH, et al. J Biol Chem 1951;163:265–275.
16.
Rhoads ML, Murrell KD, Dilling GW, Wong MM, Baker NF. A potential diagnostic reagent for bovine cysticercosis. J Parasitol 1985;71(6):779–787.
17.
Yakoleff-Greenhouse V, Flisser A, Sierra A, Larralde C. Analysis of antigenic variation in cysticerci of Taenia solium. J Parasitol 1982;68(1):39–47.
PDF Links
Full text via DOI
Download Citation 
