Cytotoxicity of resident and lymphokine-activated mouse peritoneal macrophage against <i>Trichomonas vaginalis</i>

, Ryu, J S; , Ahn, M H; , Min, D Y

doi:1990.28.2.85

Korean J Parasito > Volume 28(2):1990 > Article

Original Article


Korean J Parasitol. 1990 Jun;28(2):85-89. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1990.28.2.85
Copyright © 1990 by The Korean Society for Parasitology


Cytotoxicity of resident and lymphokine-activated mouse peritoneal macrophage against Trichomonas vaginalis
J S Ryu,M H Ahn and D Y Min
Department of Parasitology, College of Medicine, Hanyang University, Seoul 133-791, Korea.


Abstract
This study was aimed to observe the direct and lymphokine-activated cell mediated cytotoxic effects against Trichomonas vaginalis by mouse peritoneal macrophages. Cytotoxicity was measured as release of ³H-thymidine from prelabeled protozoa, and tested in U-bottom microtiter plates. A 0.1 ml suspension of labeled protozoa (2 × 10⁵/ml) was placed in each well, followed by 0.1 ml of a suspension containing increasing numbers of peritoneal cells. After a 24 hr incubation at 37℃, 0.1 ml of the supernatant was collected and counted in liquid scintillation counter. Mouse peritoneal macrophages had appreciable level of spontaneous cytotoxicity against T. vaginalis at the effector to target cell ratios from 5:1 to 50:1. Treatment of macrophages with lymphokine, produced by PHA-stimulated spleen cells, increased the cytotoxicity in comparison with resident macrophages against T. vaginalis. The degree of macrophage activation for the killing was not dependent upon the lymphokine concentration. Peritoneal cells adherent to plastic displayed significant levels of cytotoxicity against T. vaginalis. This study indicates that mouse peritoneal macrophages are spontaneously cytotoxic for T. vaginalis and lymphokine increases the cytotoxicity by activating macrophages to kill T. vaginalis.

Tables


	Table 1 Cytotoxicity of mouse macrophages against T. vaginalis in different E : T ratios and incubation times


	Table 2 Cytotoxicity of peritoneal cells against T. vaginalis


	Table 3 Cytotoxicity of lymphokine-activated macrophages from normal & immunized mice against T. vaginalis


	Table 4 Effect of lymphokine concentrations on the macrophage cytotoxicity against T. vaginalis

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