The fate of spargana inoculated into the cat brain and sequential changes of anti-sparganum IgG antibody levels in the cerebrospinal fluid

, Wang, K C; , Huh, S; , Hong, S T; , Chai, J Y; , Choi, K S; , Lee, S H

doi:1990.28.1.1

Korean J Parasito > Volume 28(1):1990 > Article

Original Article


Korean J Parasitol. 1990 Mar;28(1):1-10. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1990.28.1.1
Copyright © 1990 by The Korean Society for Parasitology


The fate of spargana inoculated into the cat brain and sequential changes of anti-sparganum IgG antibody levels in the cerebrospinal fluid
K C Wang,^S Huh,^S T Hong,J Y Chai,K S Choi,^ and S H Lee
Department of Parasitology, College of Medicine, Seoul National University, Seoul 110-460, Korea.
^*Department of Neurosurgery, College of Medicine, Seoul National University, Seoul 110-460, Korea.
^*Present address: Department of Parasitology, faculty of Medicine, Hallym University, Chunchon 200-702, Korea.


Abstract
To establish an animal model of intracranial sparganosis, the fate and behavior of the experimentally inoculated spargana were observed. A total of 102 scolices of spargana were injected into 22 cat brains, and the cats were sacrificed at 2 weeks, 1 month, 3 months and 6 months after the inoculation. Neurosparganosis was established in 77% of the cats. Of 43 recovered worms, 19 (44%) were located in the subdural or subarachnoid space, 16 (37%) in the brain parenchyme, and 2 (5%) in the lateral ventricle. One was detected at the diploic space of the skull and 5 were outside the cranial cavity. All but one were alive, and had grown tails. They were distributed in the brain parenchyme randomly. There was no place which they could not invade. No adult was found in the intestine. Cerebrospinal fluid (CSF) was collected before inoculation, 1 week, 2 weeks, 1 month, 3 months and 6 months after inoculation. The level of anti-sparganum IgG antibody in CSF measured by ELISA began to increase above the criteria of positivity 1 month after inoculation. Three months after inoculation, the values markedly increased. The present findings reveal that intracranial inoculation of spargana into the brains of cats would be a good animal model of experimental neurosparganosis.

Figures


	Fig. 1 Recovery rate of worms from each cat.

Fig. 2
Gross finding of the brain and recovered worms. A; (3 months after inoculation) a worm on the suprasylvian gyrus (arrows), B; (2 weeks after inoculation) a worm in the interhemispheric fissure (arrow), C; (3 months after inoculation) a worm beneath the temporal lobe (arrow), D; (3 months after inoculation) growing worms with tail from the subdural space, E; (2 weeks after inoculation) a worm in the thalamus and worm tracts anterior and posterior to the worm, F; (1 months after inoculation) worms in the internal capsules of both sides and tracts including worms in the brain stem and the cerebellum, G; (3 months after inoculation) a worm recovered from the scalp (arrow), H; (3 months after inoculation) an worm recovered from the diploic space of the skull (small arrows) 5mm apart from the hole made for the inoculation of the worms (large arrow).

Fig. 3
Schematic drawing of the distribution of total recovered worms. Upper; worms in the subdural/subarachnoid (IH=interhemispheric fissure, AS=anterior sigmoid gyrus, L=lateral gyrus, SSV=suprasylvian gyrus, ES=ectosylvian gyrus, OB=olfactory bulb, SS=suprasellar cistern, ST=subtemporal, SO=suboccipital), Lower; intraparenchymal worms (WM=hemispheric white matter, LV=lateral ventricle, CN=caudate nucleus, IC=internal capsule, TH=thalamus, H=hippocampal formation, BS=brain stem, CBL=cerebellum)


	Fig. 4 Results of ELISA on the cerebrospinal fluid (CSF, left) and the serum (right)

Tables


	Table 1 Number of cats used in the experiment


	Table 2 Recovery of the worms by time and location


	Table 3 Distribution of spargana in cats after inoculation


	Table 4 Results of ELISA of cerebrospinal fluid

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