Cross-reacting and specific antigenic components in cystic fluid from metacestodes of <i>Echinococcus granulosus</i> and <i>Taenia solium</i>

, Kong, Y; , Kang, S Y; , Cho, S Y; , Min, D Y

doi:1989.27.2.131

Korean J Parasito > Volume 27(2):1989 > Article

Original Article


Korean J Parasitol. 1989 Jun;27(2):131-139. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1989.27.2.131
Copyright © 1989 by The Korean Society for Parasitology


Cross-reacting and specific antigenic components in cystic fluid from metacestodes of Echinococcus granulosus and Taenia solium
Y Kong,S Y Kang,S Y Cho and D Y Min^**
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756
Department of Parasitology, College of Medicine, Hanyang University^**, Seoul 133-791, Korea.


Abstract
Sera from confirmed patients of 5 hydatidosis, 67 neurocysticercosis and 89 other parasitic diseases were tested for specific antibody (IgG) levels by ELISA to cystic fluid antigens from metacestodes of Echinococcus granulosus (HF) and Taenia solium (CF). All hydatidosis sera reacted positively to both HF and CF while neurocysticercosis sera did in 49.3% to HF and 85.1% to CF. The frequencies of cross-reactions were lower in other parasitic diseases to both antigens. By SDS-PAGE, protein bands of 64, 35, 22 and 7 kilodaltons (kDa) were found common in HF and CF. SDS-PAGE/immunoblot exhibited that hydatidosis sera reacted crossly to CF at 135, 110, 100, 86, 64, 45, 39, 35 and 24 kDa bands while neurocysticercosis sera did to HF at 135, 100, 86, 64, 52, 39, 35, 29 and 24 kDa bands. These results indicated that protein bands of 135, 100, 86, 64, 39, 35 and 24 kDa were major common components in HF and CF. Protein bands of 7 kDa in HF and 15, 10 and 7 kDa in CF did not react crossly and were specific components in respective antigens.

Figures


	Fig. 1 Specific antibody (IgG) levels to hydatid fluid (HF) as measured by ELISA in disease groups. Dotted line at abs. 0.25 is cut-off value of the positive reaction. 1. Hydatidosis, 2. Neurocysticercosis, 3. Paragonimiasis, 4. Sparganosis 5. Clonorchiasis and 6. Taenia saginata infection.


	Fig. 2 Specific antibody (IgG) levels to cystic fluid of T. solium metacestodes(CF) as measured by ELISA in disease groups.1. Hydatidosis, 2. Neurocysticercosis, 3. Paragonimiasis, 4. Sparganosis, 5. Clonorchiasis, and 6. T. saginata infection.


	Fig. 3 SDS-PAGE findings of HF and CF in 10~15% linear gradient gels at reducing condition. Coomassie brilliant blue R-250 stained. Mr: Molecular weight markers in kilodaltons (kDa).


	Fig. 4 SDS-PAGE/immunoblot findings to HF as antigen in sera of hydatidosis and neurocysticercosis patients. Mr: Molecular weight markers in kDa. Numbers in right column are those of cross-reacting components in kDa.


	Fig. 5 SDS-PAGE/immunoblot findings to CF in sera of neurocysticercosis and hydatidosis patients. Mr: Molecular weight markers in kDa. Numbers in right column are those of cross-reacting components in kDa.

Tables


	Table 1 Positive reactions of hydatidosis, neurocysticercosis and other parasitic diseases to HF and CF by ELISA

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