Study on <i>Centrocestus armatus</i> in Korea. II. Recovery rate, growth and development of worms in albino rats

, Hong, S J; , Woo, H C; , Chai, J Y; , Chung, S W; , Lee, S H; , Seo, B S

doi:1989.27.1.47

Korean J Parasito > Volume 27(1):1989 > Article

Original Article


Korean J Parasitol. 1989 Mar;27(1):47-56. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1989.27.1.47
Copyright © 1989 by The Korean Society for Parasitology


Study on Centrocestus armatus in Korea. II. Recovery rate, growth and development of worms in albino rats
S J Hong,H C Woo,J Y Chai,^S W Chung,^S H Lee,^* and B S Seo^**
Department of Parasitology, College of Medicine, Kyeong-Sang National University, Chinju 660-280, Korea.


Abstract
Some biological characteristics of Centrocestus armatus were studied using albino rats as its experimental host. The metacercariae were collected from Zacco platypus by artificial digestion method. Laboratory rats (Wistar) were fed each 100 or 200 metacercariae and sacrificed on 1, 2, 3, 4, 5, 6, 8, 14 and 28 days after infection to recover worms of various ages. The average recovery rate was 10.7% from 82 rats. The rate decreased rather slowly for the first 8 days but showed a steep decrease thereafter. Of the worms, 35.5% were recovered from the duodenum and 62.5% from the jejunum. At metacercarial stage, body length was 293 µm and body width 144 µm. At adult stage, the length and width reached 382 µm and 214 µm respectively at 14 days after infection. The testes and Mehlis' gland were recognized at metacercarial stage, whereas the ovarian analage appeared on the 1st day of infection, seminal vesicle and vitellaria on the 2nd day, and seminal receptacle and uterine eggs on the 3rd day. Until 8 days after infection the genital organs developed continuously and the number of uterine eggs increased. The above results show that albino rats are one of useful experimental hosts for C. armatus and the worms can develop to adults in 3 days after infection.

Figures

Fig. 1-6
Fig. 1. A metacercaria showing 'X'-shape excretory bladder(EB) and testes(T). Bar=50 µm.

Fig. 2. Circumoral spines around oral sucker of an excysted metacercaria. Bar=25 µm.

Fig. 3. Excysted metacercaria, acetocarmine stained, showing Mehlis' gland(M). Bar=100 µm.

Fig. 4. A 1-day old worm showing overian anlage(OA). Bar=100 µm.

Fig. 5. A 2-day old worm showing seminal vesicle(SV) with sperms. Bar=200 µm.

Fig. 6. Seminal receptacle(SR) and intrauterine eggs in a 3-day-old worm. Bar=100 µm.

Fig. 7-12
Fig. 7. Higher magnification of Fig. 6 showing sperms in seminal vesicle and receptacle. Bar=30 µm.

Fig. 8. A 6-day old worm. Bar=100µm.

Fig. 9. Circumoral spines around oral sucker of a 6-day old worm, unstained. Bar=30 µm.

Fig. 10. Intrauterine eggs in a 6-day old worm. Bar=30 µm.

Fig. 11. A 8-day old worm. Bar=100 µm.

Fig. 12. A 14-day old worm. Bar=100 µm.


	Fig. 13 Drawing of an acult C. armatus (8-day old).


	Fig. 14 Circumoral spines around aral sucker of C. armatus.

Tables


	Table 1 The recovery rate of C. armatus from experimentally infected rats


	Table 2 Measurements of C. armatus recovered from albino rats from 1 to 14 days after infection (unit : µm)

References


1.	Chai JY. Seoul J Med 1979;20(2):104–117.

2.	Chen HT. J Parasit 1942;28(4):285–298.

3.	Hong SJ, Seo BS, Lee SH, Chai JY. A human case of Centrocestus armatus infection in Korea. Korean J Parasitol 1988;26(1):55–60.

6.	Mishima T. Tokyo Iji Shinji 1959;76(5):291–299.

7.	Mansour NS, Youssef M, Awadalla HN, Hammouda NH, Boulos LM. Susceptibility of small laboratory animals to Pygidiopsis genata (Trematoda : Heterophydae). J Egypt Soc Parasitol 1981;11(1):225–234.

8.	Rhee JK, Rim MH, Baek BK, Lee HI. [Survey On Encysted Cercaria Of Trematodes From Fresh-Water Fishes In Tongjin Riverside Areas In Korea]. Korean J Parasitol 1984;22(2):190–202.

9.	Saito S. Jpn J Parasitol 1984;33(3):191–201.

*10.*	Seo BS, Chun KS, Chai JY, Hong SJ, Lee SH. Studies on intestinal trematodes in Korea: XVII. Development of egg lying capacity of Echinostoma hortense in albino rats and human experimental infection. Korean J Parasitol 1985;23(1):24–32.

*11.*	Takahashi S. Okayama Igakkai Zzasshi 1929;4(8):1759–1771.

*12.*	Tanabe H. Kyoto Igaku Zasshi 1922;19(3):239–252.