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Antibody changes in paragonimiasis patients after praziquantel treatment as observed by ELISA and immunoblot
S Y Cho,S I Kim,S Y Kang,Y Kong,S K Han,*Y S Shim,* and Y C Han*
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
Department of Internal Medicine*, Seoul National University Hospital, Seoul 110-744, Korea.
Abstract
To observe antibody changes after praziquantel treatment in paragonimiasis, a total of 46 serum samples from 13 serologically diagnosed patients was collected for 4-28 months. The specific antibody (IgG) levels were measured by enzyme-linked immunosorbent assay (ELISA). All but one patient who needed retreatment became symptom-free within a week. Antibody levels were dropped near to or below a cut-off absorbance (abs.) of 0.25 in varying intervals from 4 to 18 months. Of 9 patients who were retested within 3 months, 5 revealed temporary elevation of antibody level. After the elevation, the levels began to decline slowly to negative ranges. If treated earlier after symptoms developed, the temporary elevation did not occur and intervals to negative conversion were shorter. By sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)/immunoblot, antigen-antibody reactions in individual patient faded gradually without significant changes in reacting antigen bands.
Figures
Fig. 1 Absorbance changes before and after praziquantel treatment in paragonimiasis patients by ELISA. A-M : Patient number. Dotted band at abs. 0.25 : Criterion of positive reaction (Bf : Before the praziquantel treatment. ↓ : Praziquantel treatment).
Fig. 2 SDS-PAGE/immunoblot of paragonimiasis patients before and after praziquantel treatment. Saline extract of adult P. westermani was electrophoresed in reducing condition. A-M : Patients number. B, 0~28 at top of immunoblot: B: before treatment 0 : At the time of treatment, 1~28 : Months after the treatment. Numerals standing at left column: Molecular weight in kDa.
Tables
Table 1 Symptoms and duration of illness in the subjected patients
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