Imunological characterization of antigens from cysticercus and sparganum and their application to immunodiagnosis 1. Immunological characteristics of crude antigenic components from Cysticercus cellulosae

, Kim, Chang Hwan; , Yang, James

doi:1988.26.4.245

Korean J Parasito > Volume 26(4):1988 > Article

Original Article


Korean J Parasitol. 1988 Dec;26(4):245-254. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1988.26.4.245
Copyright © 1988 by The Korean Society for Parasitology


Imunological characterization of antigens from cysticercus and sparganum and their application to immunodiagnosis 1. Immunological characteristics of crude antigenic components from Cysticercus cellulosae
Chang Hwan Kim and James Yang^*
College of Natural Science, Gyeongsang National University, Chinju 660-701, Korea.
^*Tropical Diseases Unit, Toronto General Hospital, Toronto, Canada.


Abstract
We studied the serological reaction between various antigenic components from Cysticercus cellulosae and IgG antibodies in sera of cysticercosis, sparganosis, hydatidosis patients and normal humans by ELISA and EITB. In serological tests by ELISA, we recognized cross reaction of Cysticercus antigenic components with IgG antibodies in heterologous sera such as sparganosis and hydatidosis patients or normal humans. The crude antigenic components of Cysticercus showed lower ELISA sensitivity in homologous sera from cysticercosis patients than heterologous sera from hydatidosis patients. A total of 31 polypeptide bands with 260 KDa-22 KDa molecular weights were detected by SDS-PAGE, and 11 of them showed strong intensity. Total 22 components of them were recognized by IgG antibodies in cysticercosis patients sera. However, 12 of them were recognized also by normal human sera, 11 were by sparganosis sera, and 21 were by hydatidosis patients sera. The crude antigenic components of 104 KDa, 82 KDa, 72 KDa, 59 KDa and 34 KDa molecular weights were nonspecific ones, which cross-reacted with sera of either cysticercosis, sparganosis, hydatidosis patients or normal humans.

Figures


	Fig. 1 Extraction procedure of crude antigenic protein from Cysticercus.


	Fig. 2 Procedure of ELISA.


	Fig. 3 Resolved bands of crude antigenic protein extracted from C. cellulosae by 12% SDS-PAGE. (A: Protein was visualized by staining with Coomassie blue R-250, B: Protein was visualized by staining with silver) [HM: High molecular weight marker protein, LM: Low molecular weight marker protein, BPB: Bromophenol blue, Cy: Cysticercus crude antigenic protein]


	Fig. 4 Antigenic components from Cysticercus reacting with sera of 7 cysticercosis patients. (M: Marker protein, Cn: Serial No. of cysticercosis sera)


	Fig. 5 Antigenic components reacting with sera of 7 normal humans (control group). (M: Marker protein, Nn: Serial No. of normal sera)


	Fig. 6 Antigenic components reacting with sera of 4 sparganosis patients. (M: Marker protein, Sn: Serial No. of sparganosis sera)


	Fig. 7 Antigenic components reacting with sera of 9 hydatidosis patients. (M: Marker protein, Hn: Serial No. of hydatidosis sera)

Tables


	Table 1 The ELISA absorbance values obtained in patients sera using Cysticercus crude extract as antigen (data of 7, 5, 9 sera of each disease and 7 controls)


	Table 2 Crude antigenic proteins from Cysticercus reacting with cysticercosis, sparganosis, hydatidosis and normal sera by EITB

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