Antigenic protein fractions reacting with sera of sparganosis patients

Original Article


Korean J Parasitol. 1988 Sep;26(3):163-167. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1988.26.3.163
Copyright © 1988 by The Korean Society for Parasitology


Antigenic protein fractions reacting with sera of sparganosis patients
Sung Ho Choi,Shin Yong Kang,Yoon Kong and Seung-Yull Cho
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.


Abstract
To observe the antigenic protein fractions in saline extract of Spirometra mansoni plerocercoid (sparganum), the crude extract was separated in reducing conditions of sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE). The proteins, transferred by electrophoresis to nitrocellulose paper, were reacted with sera from 15 surgically confirmed sparganosis and 24 cysticercosis patients for immunoblotting. Out of 30 identified protein bands in the extract, bands of 29 and 36 kilodaltons (kDa) were the strongest and the most frequently reacting with specific antibody (IgG) in sparganosis sera. Bands of higher molecular weight also reacted with the sera but their frequency of reactions was lower. Sera of cysticercosis reacted with different protein bands in saline extract of sparganum, but the cross reactions were observed in strong antigenic bands of 29 and 36 kDa.

Figures


	Fig. 1 Findings of SDS-PAGE of saline extract of sparganum. SDS-PAGE was done in 10~15% linear gradient gel. The saline extract was heat-treated with sample buffer containing 10% 2-mercaptoethanol. Coomassie brilliant blue R-250 stained. M_r: Molecular weight in kDa.


	Fig. 2 SDS-PAGE/immunoblot findings in 15 confirmed human sparganosis patients to saline extract of spargana. M_r: Molecular weight in kDa.


	Fig. 3 SDS-PAGE/immunoblot findings in 24 confirmed neurocysticercosis to saline extract of spargana. Patients No. 1~6 and 23~24 cross-reacted positively by ELISA against sparganum antigen. M_r: Molecular weight in kDa.

References


1.	Chang KH, Cho SY, Chi JG, Kim WS, Han MC, Kim CW, Myung H, Choi KS. Cerebral sparganosis: CT characteristics. Radiology 1987;165(2):505–510.

2.	Cho SY, Kang SY, Kim SI. Analysis of antigen specificity using monoclonal and polyclonal antibodies to Cysticercus cellulosae by enzyme-linked immunoelectrotransfer blot technique. Korean J Parasitol 1987;25(2):159–167.

3.	Cho SY, Kim SI, Kang SY, Choi DY, Suk JS, Choi KS, Ha YS, Chung CS, Myung HJ. Evaluation of enzyme-linked immunosorbent assay in serological diagnosis of human neurocysticercosis using paired samples of serum and cerebrospinal fluid. Korean J Parasitol 1986;24(1):25–41.

4.	Cho SY. Chung-Ang J Med 1982;7:321–333.

5.	Fukase T, et al. Jpn J Parasitol 1985;34:351–360.

6.	Ishii A. Jpn J Parasitol 1973;22:75–78.

7.	Kim H, Kim SI, Cho SY. Serological Diagnosis Of Human Sparganosis By Means Of Micro-ELISA. Korean J Parasitol 1984;22(2):222–228.

8.	Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227(5259):680–685.

9.	Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265–275.

*10.*	Ohnishi Y, et al. Jpn J Parasitol 1986;35:25–33.

*11.*	Tsang VC, Peralta JM, Simons AR. Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol 1983;92:377–391.