Comparative evaluation of indirect immunofluorescent antibody test with enzyme-linked immunosorbent assay in serodiagnosis of human neurocysticercosis

Original Article


Korean J Parasitol. 1988 Mar;26(1):27-32. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1988.26.1.27
Copyright © 1988 by The Korean Society for Parasitology


Comparative evaluation of indirect immunofluorescent antibody test with enzyme-linked immunosorbent assay in serodiagnosis of human neurocysticercosis
Kee Seon Eom,Seung-Yull Cho,^* and Han Jong Rim^**
Department of Parasitology, College of Medicine, Chungbuk National University, Cheongju 360-763, Korea.
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.
Department of Parasitology, College of Medicine, Korea University, Seoul 110-522, Korea.


Abstract
The applicability of indirect immunofluorescent antibody test (IFAT) was compared with enzyme-linked immunosorbent assay (ELISA) in sera from 163 cases of confirmed neurocysticercosis, 101 other neurologic and parasitic diseases and 100 normal controls. As antigen, frozen sections of a Taenia solium metacestode from a human brain was used in IFAT and cystic fluid was used in ELISA. For the detection of specific IgG antibody, IFAT was equally sensitive (89. 6%) and specific (85. l%) as ELISA. The antibody titers by IFAT were correspondingly increased with mean absorbance of ELISA. The corresponding rate of positivity in the two techniques was 90.8%. Except for the difficulty in detecting antibodies in cerebrospinal fluid (CSF), IFAT was concluded to be very useful for the serodiagnosis of human neurocysticercosis.

Figures


	Fig. 1 Relations between absorbance by ELISA and IFAT titers in detecting specific IgG antibody in sera of 163 confirmed neurocysticercosis cases. Open circle (◦): serum (-)/CSF (+) cases by ELISA

Tables


	Table 1 Results of serum IFAT in 163 confirmed neurocysticercosis patients and 100normal control cases


	Table 2 Comparative incidence of cross reactions by ELISA and IFAT for specific IgG antibodies in sera of other neurologic and parasitic diseases

References


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