Serological cross-reactivity between <i>Sarcocystis</i> and <i>Toxoplasma</i> in pigs

, Moon, Moo Hong

doi:1987.25.2.188

Korean J Parasito > Volume 25(2):1987 > Article

Original Article


Korean J Parasitol. 1987 Dec;25(2):188-194. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1987.25.2.188
Copyright © 1987 by The Korean Society for Parasitology


Serological cross-reactivity between Sarcocystis and Toxoplasma in pigs
Moo Hong Moon
Department of Veterinary Medicine, College of Agriculture, Kyungpook National University, Daegu 635, Korea.


Abstract
The development of antibody titers and cross reation between Sarcocystis and Toxoplasma were investigated by means of IFA test and ELISA in pigs experimentally infected with 1.5 × 10(6) S. suicanis sporocysts and 10,000 T. gondii oocysts, respectively. The intact and soluble Sarcocystis antigens were prepared from the bradyzoites harvested by peptic digestion of infected pork. The intact and soluble Toxoplasma, antigens were prepared from the tachyzoites in mouse peritoneal cavity. IgG antibodies in pigs infeded with Sarcocystis and Toxoplasma, respectively were detected first at 2 weeks post infection on both IFA test and ELISA. The antibody titer to Toxoplasma reached its maximum at 6 weeks post infection and decreased thereafter. The antibody titer to Sarcocystis reached its maximum terminally. The cross-reaction titer in pigs infected with Toxoplasma against Sarcocystis antigen was up to 1:16 in IFA test and up to 1:32 in ELISA. The titer in control group was below 1:4 in both reactions.

Figures


	Fig. 1 Dynamics of IgG antibody of pigs infected with Sarcocystis (…)and Toxoplasma (-) to homologous antigens, and Toxoplasma (-•-) to Sarcocystis antigen by IFA test.


	Fig. 2 Dynamics of IgG antibody of pigs infected with Sarcocystis (…)and Toxoplasma (-) to homologous antigens, and Toxoplasma (-•-) to Sarcocystis antigen by ELISA.

References


1.	Barrows PL, Prestwood AK, Green CE. Experimental Sarcocystis suicanis infections: disease in growing pigs. Am J Vet Res 1982;43(8):1409–1412.

2.	Choi WY, Yoo JU, Kim WG. [Toxoplasma antibodies by indirect latex agglutination tests in St. Mary's Hospital patients]. Korean J Parasitol 1982;20(1):33–37.

3.	Choi WY, Yoo JE, Nam HW, Oh CY, Kim SW, Katakura K, Kobayashi A. Toxoplasma antibodies by indirect latex agglutination tests in zoo animals. Korean J Parasitol 1987;25(1):13–23.

4.	Dubey JP. A review of Sarcocystis of domestic animals and of other coccidia of cats and dogs. J Am Vet Med Assoc 1976;169(10):1061–1078.

5.	Dubey JP, Swan GV, Frenkel JK. A simplified method for isolation of Toxoplasma gondii from the feces of cats. J Parasitol 1972;58(5):1005–1006.

6.	Dubey JP, Weisbrode SE, Speer CA, Sharma SP. Sarcocystosis in goats: clinical signs and pathologic and hematologic findings. J Am Vet Med Assoc 1981;178(7):683–699.

7.	Farrell RL, Docton FL, Chamberlain DM, Cole CR. Toxoplasmosis. I. Toxoplasma isolated from swine. Am J Vet Res 1952;13(47):181–185.

8.	Fayer R, Johnson AJ, Lunde M. Abortion and other signs of disease in cows experimentally infected with Sarcocystis fusiformis from dogs. J Infect Dis 1976;134(6):624–628.

9.	Fayer R, Lunde MN. Changes in serum and plasma proteins and in IgG and IgM antibodies in calves experimentally infected with sarcocystis from dogs. J Parasitol 1977;63(3):438–442.

*10.*	Fletcher S. Indirect Fluorescent Antibody Technique In The Serology Of Toxoplasma Gondii. J Clin Pathol 1965;18:193–199.

*11.*	Gasbarre LC, Suter P, Fayer R. Humoral and cellular immune responses in cattle and sheep inoculated with Sarcocystis. Am J Vet Res 1984;45(8):1592–1596.

*12.*	Jacobs L, Lunde MN. A hemagglutination test for toxoplasmosis. J Parasitol 1957;43(3):308–314.

*13.*	Kelen AE, et al. Canad J Microbiol 1962;8:545–554.

*14.*	Mun JB. Bull Vet Res Lab 1965;11(1):19–25.

*15.*	O'Donoghue PJ, Weyreter H. Detection of Sarcocystis antigens in the sera of experimentally-infected pigs and mice by an immunoenzymatic assay. Vet Parasitol 1983;12(1):13–29.

*16.*	O'Donoghue PJ, et al. Zbl Bakt Hyg 1984;257:168–184.

*17.*	Prestwood AK, Cahoon RW, McDaniel HT. Sarcocystis infections in Georgia swine. Am J Vet Res 1980;41(11):1879–1881.

*18.*	Seneviratna P, Edward AG, DeGiusti DL. Frequency of Sarcocystis spp in Detroit, metropolitan area, Michigan. Am J Vet Res 1975;36(3):337–339.

*19.*	Suh MD, et al. Korean J Vet Res 1972;12(1):51–55.

*20.*	Suzuki T, et al. Jpn J Parasit 1980;29:33.

*21.*	Weyreter H, et al. Zbl Bakt Hyg 1982;A253:407–416.

*22.*	Weyreter H, O'Donoghue PJ, Weber M, Rommel M. Class-specific antibody responses in pigs following immunization and challenge with sporocysts of Sarcocystis miescheriana. Vet Parasitol 1984;16(3-4):201–205.