Parasites, Hosts and Diseases

"B-cell epitope"

Original Article

IgE Binding Reactivity of Peptide Fragments of Bla g 4, a Major German Cockroach Allergen: Kwang Hyun Shin, Kyoung Yong Jeong, Chein-Soo Hong, Tai-Soon Yong; Korean J Parasitol 2009;47(1):31-36.
Published online March 12, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.1.31

Abstract
PDF

Cockroaches have been recognized as a major cause of asthma. Bla g 4 is one of the most important German cockroach allergens. The aim of this study is to investigate IgE reactivity to the recombinant Bla g 4 (rBla g 4) in the sera of allergic patients and identify linear IgE binding epitope. For protein expression, full-length Bla g 4 (EF202172) was divided into 5 overlapping peptide fragments (E1: aa 1-100, E2: aa 34-77, E3: aa 74-117, E4: aa 114-156, and E5: aa 153-182). The full-length and 5 peptide fragments of Bla g 4 was generated by PCR and over-expressed in E. coli BL21 (DE3). The IgE binding reactivities of the full-length and peptide fragments were measured by ELISA using 32 serum samples of cockroach allergy. The sera of 8 patients (25%) reacted with rBla g 4. Four sera (100%) showed IgE-binding reactivity to full-length and peptide fragment 4, and 2 sera (50%) reacted with peptide fragment 2. One (20%) serum reacted with peptide fragment 3. The results of ELISA using overlapping recombinant fragments indicated that the epitope region was located at amino acid sequences 34-73 and 78-113, and major IgE epitope of Bla g 4 was located at amino acid sequences 118-152 of C-terminal. B-cell epitope analysis of German cockroach allergen Bla g 4 could contribute to the strategic development of more specific and potentially efficacious immunotherapy.

Citations

Citations to this article as recorded by

Variability in German Cockroach Extract Composition Greatly Impacts T Cell Potency in Cockroach-Allergic Donors
Giovanni Birrueta, April Frazier, Anna Pomés, Jill Glesner, Stephanie Filep, Coby Schal, Kyoung Yong Jeong, Curtis McMurtrey, Thomas Vander Schans, William H. Hildebrand, Paula Busse, Avraham Beigelman, Leonard B. Bacharier, Bjoern Peters, Alessandro Sett
Frontiers in Immunology.2019;[Epub] CrossRef
Insect (food) allergy and allergens
Steffie de Gier, Kitty Verhoeckx
Molecular Immunology.2018; 100: 82. CrossRef
Cockroach allergen exposure and risk of asthma
D. C. Do, Y. Zhao, P. Gao
Allergy.2016; 71(4): 463. CrossRef
IgE-Binding Epitope Mapping and Tissue Localization of the Major American Cockroach Allergen Per a 2
Mey-Fann Lee, Chia-Wei Chang, Pei-Pong Song, Guang-Yuh Hwang, Shyh-Jye Lin, Yi-Hsing Chen
Allergy, Asthma & Immunology Research.2015; 7(4): 376. CrossRef
Different Bla‐g T cell antigens dominate responses in asthma versus rhinitis subjects
M. B. C. Dillon, V. Schulten, C. Oseroff, S. Paul, L. M. Dullanty, A. Frazier, X. Belles, M.‐D. Piulachs, C. Visness, L. Bacharier, G. R. Bloomberg, P. Busse, J. Sidney, B. Peters, A. Sette
Clinical & Experimental Allergy.2015; 45(12): 1856. CrossRef
The major cockroach allergen Bla g 4 binds tyramine and octopamine
Lesa R. Offermann, Siew Leong Chan, Tomasz Osinski, Yih Wan Tan, Fook Tim Chew, J. Sivaraman, Yu-Keung Mok, Wladek Minor, Maksymilian Chruszcz
Molecular Immunology.2014; 60(1): 86. CrossRef
Environmental assessment and exposure reduction of cockroaches: A practice parameter
Jay Portnoy, Ginger L. Chew, Wanda Phipatanakul, P. Brock Williams, Carl Grimes, Kevin Kennedy, Elizabeth C. Matsui, J. David Miller, David Bernstein, Joann Blessing-Moore, Linda Cox, David Khan, David Lang, Richard Nicklas, John Oppenheimer, Christopher
Journal of Allergy and Clinical Immunology.2013; 132(4): 802. CrossRef
Identification of Novel Allergenic Components from German Cockroach Fecal Extract by a Proteomic Approach
Kyoung Yong Jeong, Chung-ryul Kim, Jina Park, In-Soo Han, Jung-Won Park, Tai-Soon Yong
International Archives of Allergy and Immunology.2013; 161(4): 315. CrossRef
The Cockroach and Allergic Diseases
Myung Hyun Sohn, Kyu-Earn Kim
Allergy, Asthma & Immunology Research.2012; 4(5): 264. CrossRef
Der-p2 (Dermatophagoides pteronyssinus) allergen-like protein from the hard tickIxodes ricinus– a novel member of ML (MD-2-related lipid-recognition) domain protein family
J. HORÁČKOVÁ, N. RUDENKO, M. GOLOVCHENKO, L. GRUBHOFFER
Parasitology.2010; 137(7): 1139. CrossRef
Household Arthropod Allergens in Korea
Tai-Soon Yong, Kyoung Yong Jeong
The Korean Journal of Parasitology.2009; 47(Suppl): S143. CrossRef