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"Chalobol Wongsawad"

Original Articles

Morphological and molecular characteristics of Paralecithodendrium longiforme (Digenea: Lecithodendriidae) adults and cercariae from Chinese pipistrelle bats and viviparid snails in Thailand
Thitichai Arttra, Pheravut Wongsawad, Chalobol Wongsawad, Nattawadee Nantarat, Preeyaporn Butboonchoo, Jong-Yil Chai
Parasites Hosts Dis 2024;62(1):85-97.
Published online February 23, 2024
DOI: https://doi.org/10.3347/PHD.23106
This study aimed to describe the morphological and molecular characteristics of Paralecithodendrium longiforme (Digenea: Lecithodendriidae) adults and cercariae isolated in Thailand. Adult flukes were isolated from the Chinese pipistrelle bat (Hypsugo sp.), and cercariae were detected in the viviparid snail (Filopaludina martensi martensi) from Chiang Mai province. The morphological characteristics were observed and described using conventional methods, and the molecular characteristics with internal transcribed spacer 2 (ITS2) and 28S rDNA gene sequences. The adult flukes were fusiform, 0.84–0.98 mm in length, and 0.37–0.49 mm in width, and were distinguishable from other species by the presence of longitudinal uterine coils. The cercariae were nonvirgulate xiphidiocercariae, with the oral sucker bigger than the acetabulum, the tail without fin fold, a body size of 117.5–138.3 × 48.3–52.2 µm, and a tail size of 100.7–103.7 × 15.0–18.9 µm. Molecular studies revealed that the adults and cercariae shared 99.3% (ITS2) and 99.6% (28S rDNA) homology with each other. They were phylogenetically close to P. longiforme with an identity of 94.5% for ITS2 and 98.7% for 28S rDNA. This study provides new information on the natural definitive host and first intermediate host of P. longiforme in Thailand. The discovery of its cercarial stage in Filopaludina snails highlights the importance of monitoring the associated second intermediate host and prevention and control of this potentially zoonotic trematode.

Citations

Citations to this article as recorded by  Crossref logo
  • Bithyniid snails (Gastropoda: Bithyniidae) infected with Xiphidiocercariae in Thailand include a new record of Bithynia siamensis siamensis as the intermediate host of Plagiorchis and Paralecithodendrium
    Abdulhakam Dumidae, Jiranun Ardpairin, Supawan Pansri, Chanatinat Homkaew, Mayura Nichitcharoen, Aunchalee Thanwisai, Apichat Vitta, Hudson Alves Pinto
    PLOS ONE.2025; 20(2): e0317052.     CrossRef
  • 4,321 View
  • 107 Download
  • 1 Web of Science
  • Crossref
Morphology and Molecular Identification of Echinostoma revolutum and Echinostoma macrorchis in Freshwater Snails and Experimental Hamsters in Upper Northern Thailand
Preeyaporn Butboonchoo, Chalobol Wongsawad, Pheravut Wongsawad, Jong-Yil Chai
Korean J Parasitol 2020;58(5):499-511.
Published online October 22, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.5.499
Echinostome metacercariae were investigated in freshwater snails from 26 districts in 7 provinces of upper northern Thailand. The species identification was carried out based on the morphologies of the metacercariae and adult flukes harvested from experimental hamsters, and on nucleotide sequences of internal transcribed spacer 2 (ITS2) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (nad1) genes. Twenty-four out of 26 districts were found to be infected with echinostome metacercariae in freshwater snails with the prevalence of 40.4%. The metacercariae were found in all 6 species of snails, including Filopaludina martensi martensi (21.9%), Filopaludina doliaris (50.8%), F. sumatrensis polygramma (61.3%), Bithynia siamensis siamensis (14.5%), Bithynia pulchella (38.0%), and Anenthome helena (4.9%). The echinostome metacercariae found in these snails were identified as Echinostoma revolutum (37-collar-spined) and Echinostoma macrorchis (45-collar-spined) morphologically and molecularly. The 2-week-old adult flukes of E. revolutum revealed unique features of the cirrus sac extending to middle of the ventral sucker and smooth testes. E. macrorchis adults revealed the cirrus sac close to the right lateral margin of the ventral sucker and 2 large and elliptical testes with slight indentations and pointed posterior end of the posterior testis. The ITS2 and nad1 sequences confirmed the species identification of E. revolutum, and the sequences of E. macrorchis have been deposited for the first time in Gen-Bank. The presence of the life cycle of E. macrorchis is a new record in Thailand and the snail F. doliaris as their second intermediate host seems to be new among the literature.

Citations

Citations to this article as recorded by  Crossref logo
  • Filling the void: Morphological and molecular phylogenetic analyses of helminths assemblage from the Egyptian egret Bubulcus ibis
    Nada A. Ibrahim, Ismail M. Shalaby, Mahi A. Ghobashy, Azza A. Taeleb, Elham A. Elkhawass
    Parasitology International.2025; 104: 102972.     CrossRef
  • The Nuclear Ribosomal Transcription Units of Two Echinostomes and Their Taxonomic Implications for the Family Echinostomatidae
    Yu Cao, Ye Li, Zhong-Yan Gao, Bo-Tao Jiang
    Biology.2025; 14(8): 1101.     CrossRef
  • Prevalence of Foodborne Trematodes in Small Indigenous Fish Species in Local Markets of Phnom Penh, Cambodia
    Domenico Caruso, Lin Kong, Samnang Keo, Sreyni Yoeurn, Samphal Seng
    Vector-Borne and Zoonotic Diseases.2024; 24(3): 141.     CrossRef
  • Morphological and molecular characteristics of Paralecithodendrium longiforme (Digenea: Lecithodendriidae) adults and cercariae from Chinese pipistrelle bats and viviparid snails in Thailand
    Thitichai Arttra, Pheravut Wongsawad, Chalobol Wongsawad, Nattawadee Nantarat, Preeyaporn Butboonchoo, Jong-Yil Chai
    Parasites, Hosts and Diseases.2024; 62(1): 85.     CrossRef
  • Development and utilization of a visual loop-mediated isothermal amplification coupled with a lateral flow dipstick (LAMP-LFD) assay for rapid detection of Echinostomatidae metacercaria in edible snail samples
    Wasin Panich, Phonkawin Jaruboonyakorn, Awika Raksaman, Thanawan Tejangkura, Thapana Chontananarth
    International Journal of Food Microbiology.2024; 418: 110732.     CrossRef
  • Genetic diversity and population structure of the helminths intermediate host Anentome helena (von dem Busch, 1847) in northern Thailand
    Nithinan Chomchoei, Chalobol Wongsawad, Preeyaporn Butboonchoo, Nattawadee Nantarat
    Asian Pacific Journal of Tropical Medicine.2024; 17(12): 536.     CrossRef
  • Morphological and DNA analyses reveal cryptic diversity in Anentome wykoffi (Brandt, 1974) (Gastropoda: Nassariidae), with descriptions of two new species from Thailand
    Nithinan Chomchoei, Thierry Backeljau, Piyatida Pimvichai, Ting Hui Ng, Nattawadee Nantarat, Gonzalo Giribet
    Invertebrate Systematics.2023; 37(11): 755.     CrossRef
  • High diversity of trematode metacercariae that parasitize freshwater gastropods in Bangkok, Thailand, and their infective situations, morphologies and phylogenetic relationships
    Pichit Wiroonpan, Thapana Chontananarth, Jong-Yil Chai, Watchariya Purivirojkul
    Parasitology.2022; 149(7): 913.     CrossRef
  • Unwanted ‘hitchhikers’ of ornamental snails: a case report of digeneans transported via the international pet trade
    A. Stanicka, R. Maciaszek, A. Cichy, J. Templin, W. Świderek, E. Żbikowska, A. M. Labecka
    The European Zoological Journal.2022; 89(1): 601.     CrossRef
  • Morphological and molecular characterization of larval trematodes infecting the assassin snail genusAnentomein Thailand
    N. Chomchoei, T. Backeljau, B. Segers, C. Wongsawad, P. Butboonchoo, N. Nantarat
    Journal of Helminthology.2022;[Epub]     CrossRef
  • 10,043 View
  • 234 Download
  • 10 Web of Science
  • Crossref
Morphological and Molecular Characteristics of Anisakis typica Larvae in Two Species of Threadfin Bream, Nemipterus hexodon and N. japonicus, from the Gulf of Thailand
Rattanachai Tunya, Chalobol Wongsawad, Pheravut Wongsawad, Jong-Yil Chai
Korean J Parasitol 2020;58(1):15-25.
Published online February 29, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.1.15
The third stage larvae (L3) of Anisakis typica were detected in 2 species of threadfin bream, Nemipterus hexodon and N. japonicus, from the Gulf of Thailand, and were morphologically and molecularly characterized. Total 100 threadfin breams, 50 Nemipterus hexodon and 50 N. japonicus, were examined with naked eyes after the opening of abdominal cavity with scissors. Almost all infected larvae remained alive and active even the fish were transported for 1-2 days. Anisakid larvae were exclusively distributed in the body cavity and rarely in the liver. The prevalence of A. typica L3 were 68.0% and 60.0% in N. hexodon and N. japonicus and their infection intensities were 3.5 and 4.2 per fish infected each. Morphological and morphometric analysis were performed by viewing specimens under both a light microscope and a scanning electron microscope. Interestingly, the protruded mucron of Anisakis typica under SEM showed a distinct cylindrical shape that differed from the cone shape of A. simplex. The protruded mucron could be used to identify A. typica L3 larvae in the future. A comparison of the ITS1-5.8S-ITS2 rDNA nucleotide sequences of these species revealed high blast scores with A. typica. Conclusively, it was confirmed that A. typica L3 are prevalent in threadfin breams from the Gulf of Thailand, and their morphological and molecular characters are something different from those of other anisakid larvae, including A. simplex and A. pegreffii.

Citations

Citations to this article as recorded by  Crossref logo
  • Invasion dynamics and ecological impacts of Anisakis typica in commercial fish from the Western Pacific Ocean
    Dhito Dwi Pramardika, Fadjar Satrija, Sulistiono Sulistiono, Risa Tiuria, Arifin Budiman Nugraha
    Veterinary World.2025; : 1365.     CrossRef
  • HOST SPECIFICITY, INFECTION DYNAMICS, AND ALLERGENICITY IN Anisakis SPP. INFESTATION: A REVIEW
    RUO YI LAM , MING SHE SEE , FAIZAH SHAROM-HARRISON, HAZLINA AHAMAD ZAKERI, NOR OMAIMA HARUN
    Universiti Malaysia Terengganu Journal of Undergraduate Research.2024; 6(2): 62.     CrossRef
  • Morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from Red Sea coral trout, Plectropomus areolatus
    Nesma Abbas Mostafa, Fathy Abdel-Ghaffar, Hamed Omar Fayed, Ayat Adel Hassan
    Parasitology Research.2023; 122(3): 705.     CrossRef
  • Morphological and Molecular Characterization of Anisakid Nematode Larvae (Nematoda: Anisakidae) in the Black Cusk eel Genypterus maculatus from the Southeastern Pacific Ocean off Peru
    Jhon Darly Chero, Luis Ñacari, Celso Luis Cruces, David Fermín Lopez, Edson Cacique, Ruperto Severino, Jorge Lopez, José Luis Luque, Gloria Saéz
    Diversity.2023; 15(7): 820.     CrossRef
  • Occurrence of Anisakis larvae in Atlantic horse mackerel (Trachurus trachurus) sold in the city of Marrakesh, Morocco
    Abdelkader Biary, Salma Berrouch, Oussama Dehhani, Abdelmalek Maarouf, Pierre Sasal, Brahim Mimouni, Jamaleddine Hafid
    Regional Studies in Marine Science.2023; 67: 103189.     CrossRef
  • Anisakiasis in the Upper Esophagus: A Case Report
    Eiji Kamba, Takashi Murakami, Hiroya Ueyama, Tomoyoshi Shibuya, Mariko Hojo, Ken Yamaji, Akihito Nagahara
    Medicina.2023; 59(10): 1888.     CrossRef
  • Ascaridoid nematodes infecting commercially important marine fish and squid species from Bangladesh waters in the Bay of Bengal
    Miguel Bao, Paolo Cipriani, Lucilla Giulietti, Mohammad Ashraful Alam, Marialetizia Palomba, Simonetta Mattiucci, Arne Levsen
    Food and Waterborne Parasitology.2022; 27: e00157.     CrossRef
  • Intraspecific Genetic Variation of Anisakis typica in Indian Mackerel Caught from the Gulf of Thailand, Samut Songkhram Province
    Tanawat Chaiphongpachara, Poom Adisakwattana, Nantana Suwandittakul, Daiji Endoh
    The Scientific World Journal.2022; 2022: 1.     CrossRef
  • Prevalence and Molecular Characterization of Ascaridoid Parasites of Philippine Decapterus species
    Tres Tinna M. Dela Cruz, Kennesa Klariz R. Llanes, Joliesa Mae S. Toledo, Junard A. Catabay, Raffy Jay C. Fornillos, Ian Kendrich C. Fontanilla, Vachel Gay V. Paller
    Journal of Nematology.2022;[Epub]     CrossRef
  • Anisakid nematodes in Trichiurus lepturus and Saurida undosquamis (Teleostea) from the South-West Indian Ocean: Genetic evidence for the existence of sister species within Anisakis typica (s.l.), and food-safety considerations
    Paolo Cipriani, Lucilla Giulietti, Salome Daniel Shayo, Julia E. Storesund, Miguel Bao, Marialetizia Palomba, Simonetta Mattiucci, Arne Levsen
    Food and Waterborne Parasitology.2022; 28: e00177.     CrossRef
  • First morphological and molecular identification of third-stage larvae of Anisakis typica (Nematoda: Anisakidae) from marine fishes in Vietnamese water
    Hoang Van Hien, Bui Thi Dung, Ha Duy Ngo, Pham Ngoc Doanh
    Journal of Nematology.2021; 53(1): 1.     CrossRef
  • First report on molecular identification of Anisakis simplex in Oncorhynchus nerka from the fish market, with taxonomical issues within Anisakidae
    Alina E. Safonova, Anastasia N. Voronova, Konstantin S. Vainutis
    Journal of Nematology.2021; 53(1): 1.     CrossRef
  • Morphological study and molecular epidemiology of Anisakis larvae in mackerel fish
    Vipavinee Cheypanya, Pheravut Wongsawad, Chalobol Wongsawad, Nattawadee Nantarat
    Asian Pacific Journal of Tropical Medicine.2021; 14(5): 214.     CrossRef
  • 10,664 View
  • 227 Download
  • 12 Web of Science
  • Crossref
Morphological and Molecular Identification of Stellantchasmus dermogenysi n. sp. (Digenea: Heterophyidae) in Thailand
Chalobol Wongsawad, Nattawadee Nantarat, Pheravut Wongsawad, Preeyaporn Butboonchoo, Jong-Yil Chai
Korean J Parasitol 2019;57(3):257-264.
Published online June 30, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.3.257
We tried a series of morphological and molecular approaches to identify a new species of Stellantchasmus (Digenea: Heterophyidae) originating from the wrestling half-beaked fish, Dermogenys pusillus of Thailand. Adult worm samples of the new species were recovered from hamsters experimentally infected with the metacercariae from D. pusillus in Thailand. Two isolates (Thai and Korean) of Stellantchasmus falcatus were used as comparative control groups. Worm samples of 3 Stellantchasmus groups were morphologically observed and molecularly analyzed with the mitochondrial cytochrome c oxidase 1 gene. The morphological characteristics of S. dermogenysi n. sp. are similar to S. falcatus originating from brackish water fish, but minor difference was noted including the absence of the prepharynx, position of the ovary near the ceca end, smaller body size, and shorter esophageal length. A phylogenetic tree derived from neighborjoining and maximum-likelihood methods suggests that S. dermogenysi n. sp. is separated from S. falcatus supported by high bootstrap values. The relative divergences persist between these host-specific trematodes, which we suggest should be recognized as 2 distinct species. Comparisons of S. dermogenysi n. sp. with S. falcatus isolated from mullets in Thailand and Korea indicate a genetic divergence of mitochondrial DNA of 19.4% and 21.7%, respectively. By the present study, a new species, Stellantchasmus dermogenysi n. sp. (Digenea: Heterophyidae), is proposed in Thailand based on molecular evidences, in addition to minor morphological differences between S. falcatus and the new species.

Citations

Citations to this article as recorded by  Crossref logo
  • Morphological and molecular characterization of larval trematodes infecting the assassin snail genusAnentomein Thailand
    N. Chomchoei, T. Backeljau, B. Segers, C. Wongsawad, P. Butboonchoo, N. Nantarat
    Journal of Helminthology.2022;[Epub]     CrossRef
  • Infections of Digenetic Trematode Metacercariae in Wrestling Halfbeak, Dermogenys pusilla from Bangkok Metropolitan Region in Thailand
    Laddawan Patarwut, Thapana Chontananarth, Jong-Yil Chai, Watchariya Purivirojkul
    The Korean Journal of Parasitology.2020; 58(1): 27.     CrossRef
  • 7,930 View
  • 169 Download
  • 2 Web of Science
  • Crossref
New Record of Thapariella anastomusa (Trematoda: Thapariellidae) Metacercariae in Northern Thailand
Waraporn Phalee, Anawat Phalee, Chalobol Wongsawad
Korean J Parasitol 2018;56(1):49-52.
Published online February 28, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.1.49
The family Thapariellidae has been reported in only 3 countries since 1990. The
objective
of this study was to identify Thapariella anastomusa metacercariae in snails in Thailand based on morphological traits using a light (LM) and a scanning electron microscope (SEM). A total of 94 Filopaludina snails were collected and identified as 50 F. martensi martensi and 44 F. doliaris. Metacercariae of T. anastomusa were recovered from the snails by the crushing method. The overall prevalence was 22.3% (21/94), and the mean intensity was 17.0 per snail. The prevalence in F. martensi martensi was 24.0% (12/50) and F. doliaris 20.5% (9/44) with the mean intensity of 18.8 and 14.8 per snail, respectively. SEM revealed traits such as a concave ventral body and well-developed oral and ventral suckers. This study represents the first report of T. anastomusa in South East Asia. While LM and SEM observations provide novel insights into T. anastomusa metacercarial morphology and life history, the trematode’s life cycle remains unclear. To date, there has been no report of T. anastomusa causing infections in humans. However, the snails F. martensi martensi and F. doliaris carrying the infective stages of T. anastomosa are frequently consumed by Thai people. This consumption, particularly uncooked snails, may present a risk of Thapariella infections in humans.

Citations

Citations to this article as recorded by  Crossref logo
  • Aestivation and its impact on the survival of snail intermediate hosts and trematode transmission in rice paddies
    K. Chantima, K. Suk-ueng, T. Tananoi, T. Prasertsin
    Journal of Helminthology.2025;[Epub]     CrossRef
  • High diversity of trematode metacercariae that parasitize freshwater gastropods in Bangkok, Thailand, and their infective situations, morphologies and phylogenetic relationships
    Pichit Wiroonpan, Thapana Chontananarth, Jong-Yil Chai, Watchariya Purivirojkul
    Parasitology.2022; 149(7): 913.     CrossRef
  • Diversity of digenean trematode larvae in snails from Lake Victoria, Kenya: First reports and bioindicative aspects
    James Omondi Outa, Helmut Sattmann, Martina Köhsler, Julia Walochnik, Franz Jirsa
    Acta Tropica.2020; 206: 105437.     CrossRef
  • Snail-borne zoonotic trematodes in edible viviparid snails obtained from wet markets in Northern Thailand
    K. Chantima, C. Rika
    Journal of Helminthology.2020;[Epub]     CrossRef
  • Freshwater Snail Diversity in Mae Lao Agricultural Basin (Chiang Rai, Thailand) with a Focus on Larval Trematode Infections
    Kittichai Chantima, Krittawit Suk-ueng, Mintra Kampan
    The Korean Journal of Parasitology.2018; 56(3): 247.     CrossRef
  • 10,155 View
  • 169 Download
  • 5 Web of Science
  • Crossref

Brief Communication

Prevalence of Centrocestus formosanus Metacercariae in Ornamental Fish from Chiang Mai, Thailand, with Molecular Approach Using ITS2
Atcharaphan Wanlop, Chalobol Wongsawad, Pongphol Prattapong, Pheravut Wongsawad, Thapana Chontananarth, Jong-Yil Chai
Korean J Parasitol 2017;55(4):445-449.
Published online August 31, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.4.445
The prevalence of Centrocestus formosanus metacercariae was investigated in ornamental fish purchased from a pet shop in Chiang Mai, Thailand, including Carassius auratus (goldfish), Cyprinus carpio (Koi), Poecilia latipinna (Sailfin Molly), Danio rerio (Zebrafish), and Puntigrus tetrazona (Tiger barb). The parasite species was identified by the morphology of worms as well as by a molecular approach using ITS2. The results showed that 50 (33.3%) of 150 fish examined were infected with the metacercariae. The highest prevalence was found in C. auratus (83.3%), and the highest intensity was noted in C. carpio (70.8 metacercariae/fish). The most important morphological character was the presence of 32-34 circumoral spines on the oral sucker. The phylogenetic studies using the rRNA ITS2 region revealed that all the specimens of C. formosanus in this study were grouped together with C. formosanus in GenBank database. This is the first report on ornamental fish, C. carpio, P. latipinna, D. rerio, and P. tetrazona, taking the role of second intermediate hosts of C. formosanus in Thailand. Prevention and control of metacercarial infection in ornamental fish is urgently needed.

Citations

Citations to this article as recorded by  Crossref logo
  • Loop‐Mediated Isothermal Amplification Combined With Lateral‐Flow Dipstick for Detection of Centrocestus formosanus in Ornamental Fish
    Metawee Sabaijai, Thanawan Tejangkura, Thapana Chontananarth
    Journal of Fish Diseases.2025;[Epub]     CrossRef
  • Molecular detection of the zoonotic trematode Centrocestus formosanus (Nishigori, 1924) (Opisthorchiida, Heterophyidae) in Central Europe
    Ľubomír Šmiga, Júlia Šmigová, Federica Berrilli, Ingrid Papajová, Peter Lazár, Isabel Guadano-Procesi
    Veterinary Research Communications.2025;[Epub]     CrossRef
  • Good practices in the rearing and maintenance of zebrafish (Danio rerio) in Brazilian laboratories
    Mateus Tavares Kütter, Leonardo José Gil Barcellos, Robert Tew Boyle, Luis Fernando Marins, Tony Silveira
    Ciência Animal Brasileira.2023;[Epub]     CrossRef
  • Boas práticas na criação e manutenção de zebrafish (Danio rerio) em laboratório no Brasil
    Mateus Tavares Kütter, Leonardo José Gil Barcellos, Robert Tew Boyle, Luis Fernando Marins, Tony Silveira
    Ciência Animal Brasileira.2023;[Epub]     CrossRef
  • Multiplex PCR development for the simultaneous and rapid detection of two pathogenic flukes, Dactylogyrus spp. and Centrocestus formosanus, in ornamental fishes
    Phonkawin Jaruboonyakorn, Thanawan Tejangkura, Thapana Chontananarth
    Aquaculture.2022; 548: 737660.     CrossRef
  • General overview of the current status of human foodborne trematodiasis
    Jong-Yil Chai, Bong-Kwang Jung
    Parasitology.2022; 149(10): 1262.     CrossRef
  • First Record ofClinostomumsp. (Digenea: Clinostomidae) inDanio rerio(Actinopterygii: Cyprinidae) and the Implication of Using Zebrafish from Pet Stores on Research
    Tony Silveira, Mateus T. Kütter, Camila M.G. Martins, Luis Fernando Marins, Robert T. Boyle, Vinicius F. Campos, Mariana H. Remião
    Zebrafish.2021; 18(2): 139.     CrossRef
  • Foodborne intestinal flukes: A brief review of epidemiology and geographical distribution
    Jong-Yil Chai, Bong-Kwang Jung
    Acta Tropica.2020; 201: 105210.     CrossRef
  • Infections of Digenetic Trematode Metacercariae in Wrestling Halfbeak, Dermogenys pusilla from Bangkok Metropolitan Region in Thailand
    Laddawan Patarwut, Thapana Chontananarth, Jong-Yil Chai, Watchariya Purivirojkul
    The Korean Journal of Parasitology.2020; 58(1): 27.     CrossRef
  • Diagnosis of Centrocestus formosanus Infection in Zebrafish (Danio rerio) in Italy: A Window to a New Globalization-Derived Invasive Microorganism
    Antonino Pace, Ludovico Dipineto, Serena Aceto, Maria Concetta Censullo, Maria Carmen Valoroso, Lorena Varriale, Laura Rinaldi, Lucia Francesca Menna, Alessandro Fioretti, Luca Borrelli
    Animals.2020; 10(3): 456.     CrossRef
  • Delineating the origins of the multidrug-resistant pathogens in ornamental fish farms by multilocus sequence typing and identification of a novel multidrug-resistant plasmid
    Songzhe Fu, Ping Ni, Yi Wang, Shibo Jin, Zhiqiang Jiang, Shigen Ye, Ruijun Li
    Canadian Journal of Microbiology.2019; 65(8): 551.     CrossRef
  • Evidence of Centrocestus formosanus (Nishigori, 1924) in Zebrafish (Danio rerio)
    Carmelo Iaria, Sergio Migliore, Daniele Macri, Maurizio Bivona, Fabiano Capparucci, Gabriella Gaglio, Fabio Marino
    Zebrafish.2019; 16(6): 522.     CrossRef
  • The life cycle of a zoonotic parasite reassessed: Experimental infection of Melanoides tuberculata (Mollusca: Thiaridae) with Centrocestus formosanus (Trematoda: Heterophyidae)
    Hudson A. Pinto, Nicole Q. Gonçalves, Danimar López-Hernandez, Eduardo A. Pulido-Murillo, Alan L. Melo, Petr Heneberg
    PLOS ONE.2018; 13(4): e0194161.     CrossRef
  • 8,846 View
  • 161 Download
  • 16 Web of Science
  • Crossref

Original Articles

Molecular Phylogenetics of Centrocestus formosanus (Digenea: Heterophyidae) Originated from Freshwater Fish from Chiang Mai Province, Thailand
Chalobol Wongsawad, Pheravut Wongsawad, Kom Sukontason, Worawit Maneepitaksanti, Nattawadee Nantarat
Korean J Parasitol 2017;55(1):31-37.
Published online February 28, 2017
DOI: https://doi.org/10.3347/kjp.2017.55.1.31
This study aimed to investigate the morphology and reconstruct the phylogenetic relationships of Centrocestus formosanus originating from 5 species of freshwater fish, i.e., Esomus metallicus, Puntius brevis, Anabas testudineus, Parambassis siamensis, and Carassius auratus, in Chiang Mai province, Thailand. Sequence-related amplified polymorphism (SRAP) and phylogeny based on internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (CO1) were performed. The results showed similar morphologies of adult C. formosanus from day 5 after infection in chicks. C. formosanus originated from 4 species of freshwater fish had the same number of circumoral spines on the oral sucker, except for those from C. auratus which revealed 34 circumoral spines. The phylogenetic tree obtained from SRAP profile and the combination of ITS2 and CO1 sequence showed similar results that were correlated with the number of circumoral spines in adult worms. Genetic variability of C. formosanus also occurred in different species of freshwater fish hosts. However, more details of adult worm morphologies and more sensitive genetic markers are needed to confirm the species validity of C. formosanus with 34 circumoral spines originating from C. auratus in the future.

Citations

Citations to this article as recorded by  Crossref logo
  • Loop‐Mediated Isothermal Amplification Combined With Lateral‐Flow Dipstick for Detection of Centrocestus formosanus in Ornamental Fish
    Metawee Sabaijai, Thanawan Tejangkura, Thapana Chontananarth
    Journal of Fish Diseases.2025;[Epub]     CrossRef
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    The Korean Journal of Parasitology.2017; 55(4): 445.     CrossRef
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Morphology and Molecular Phylogeny of Raillietina spp. (Cestoda: Cyclophyllidea: Davaineidae) from Domestic Chickens in Thailand
Preeyaporn Butboonchoo, Chalobol Wongsawad, Amnat Rojanapaibul, Jong-Yil Chai
Korean J Parasitol 2016;54(6):777-786.
Published online December 31, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.6.777
Raillietina species are prevalent in domestic chickens (Gallus gallus domesticus) in Phayao province, northern Thailand. Their infection may cause disease and death, which affects the public health and economic situation in chicken farms. The identification of Raillietina has been based on morphology and molecular analysis. In this study, morphological observations using light (LM) and scanning electron microscopies (SEM) coupled with molecular analysis of the internal transcribed spacer 2 (ITS2) region and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) gene were employed for precise identification and phylogenetic relationship studies of Raillietina spp. Four Raillietina species, including R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp., were recovered in domestic chickens from 4 districts in Phayao province, Thailand. LM and SEM observations revealed differences in the morphology of the scolex, position of the genital pore, number of eggs per egg capsule, and rostellar opening surface structures in all 4 species. Phylogenetic relationships were found among the phylogenetic trees obtained by the maximum likelihood and distance-based neighbor-joining methods. ITS2 and ND1 sequence data recorded from Raillietina sp. appeared to be monophyletic. The query sequences of R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp. were separated according to the different morphological characters. This study confirmed that morphological studies combined with molecular analyses can differentiate related species within the genus Raillietina in Thailand.

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Developmental and Phylogenetic Characteristics of Stellantchasmus falcatus (Trematoda: Heterophyidae) from Thailand
Pralongyut Sripalwit, Chalobol Wongsawad, Thapana Chontananarth, Somboon Anuntalabhochai, Pheravut Wongsawad, Jong-Yil Chai
Korean J Parasitol 2015;53(2):201-207.
Published online April 22, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.2.201
This study aimed to investigate the infection status, worm development, and phylogenetic characteristics of the intestinal trematode, Stellantchasmus falcatus. The metacercariae of S. falcatus were detected only in the half-beak (Dermogenus pusillus) out of the 4 fish species examined. Their prevalence was 90.0%, and the intensity of infection was 919 metacercariae on average. Worms were recovered from 33 (97.1%) of 34 chicks that were experimentally infected with 200 S. falcatus metacercariae each, and the average recovery rate was 43.0%. The body size and inner organs of S. falcatus quickly increased in the experimental chicks over days 1-2 post-infection (PI). In addition, ITS2 sequence data of this parasite were analyzed to examine the phylogenetic relationships with other trematodes using the UPGMA method. The results indicated that the ITS2 sequence data recorded from trematodes in the family Heterophyidae appeared to be monophyletic. This study concluded that D. pusillus serves as a compatible second intermediate host of S. falcatus in Thailand and that S. falcatus can develop rapidly in the experimental chicks. Data collected from this study can help to close the gap in knowledge regarding the epidemiology, biology, and phylogenetic characteristics of S. falcatus in Thailand.

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    The Korean Journal of Parasitology.2020; 58(1): 27.     CrossRef
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    The Korean Journal of Parasitology.2019; 57(3): 257.     CrossRef
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Experimental Life History and Biological Characteristics of Fasciola gigantica (Digenea: Fasciolidae)
Anawat Phalee, Chalobol Wongsawad, Amnat Rojanapaibul, Jong-Yil Chai
Korean J Parasitol 2015;53(1):59-64.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.59

This study was conducted to investigate the life history, morphology, and maturation of larval stages and adult worms of Fasciola gigantica in experimental mice. Lymnaea auricularia rubiginosa was used as the intermediate host, and Oryza sativa was used for encystment of the metacercariae, while Mus musculus was used as the definitive host for maturation study. Fresh eggs from the gall bladder of water buffaloes fully developed into embryonated ones and hatched out at days 11-12 after incubation at about 29ºC. Free-swimming miracidia rapidly penetrated into the snail host, and gradually developed into the next larval stages; sporocyst, redia, and daughter redia with cercariae. Fully-developed cercariae were separated from the redia and shed from the snails on day 39 post-infection (PI). Free-swimming cercariae were immediately allowed to adhere to rice plants, and capsules were constructed to protect metacercariae on rice plants. Juvenile worms were detected in intestines of mice at days 3 and 6 PI, but they were found in the bile duct from day 9 PI. Juvenile and adult flukes were recovered from 16 mice experimentally infected with metacercariae, with the average recovery rate of 35.8%. Sexually mature adult flukes were recovered from day 42 PI. It could be confirmed that experimentally encysted metacercariae could infect and develop to maturity in the experimental host. The present study reports for the first time the complete life history of F. gigantica by an experimental study in Thailand. The obtained information can be used as a guide for prevention, elimination, and treatment of F. gigantica at environment and in other hosts.

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Echinostoma revolutum: Freshwater Snails as the Second Intermediate Hosts in Chiang Mai, Thailand
Kittichai Chantima, Jong-Yil Chai, Chalobol Wongsawad
Korean J Parasitol 2013;51(2):183-189.
Published online April 25, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.2.183

The occurrence of 37-collar spined echinostome metacercariae in freshwater snails was investigated in 6 districts of Chiang Mai Province, Thailand, from October 2011 to April 2012. A total of 2,914 snails that belong to 12 species were examined, and 7 snail species (Clea helena, Eyriesia eyriesi, Bithynia funiculata, Bithynia siamensis siamensis, Filopaludina doliaris, Filopaludina sumatrensis polygramma, and Filopaludina martensi martensi) were found infected with echinostome metacercariae. The prevalence of metacercariae was the highest in Filopaludina spp. (38.5-58.7%) followed by B. funiculata (44.0%), E. eyriesi (12.5%), B. siamensis siamensis (8.2%), and C. helena (5.1%). Metacercariae were experimentally fed to hamsters and domestic chicks, and adult flukes were recovered from both hosts at days 15 and 20 post-infection. The adult flukes were identified based on morphological features, morphometrics, host-parasite relationships, and geographical distribution. They were compatible to Echinostoma revolutum or Echinostoma jurini, with only minor differences. As the adults were recovered from both hamsters and chicks, our specimens were more compatible to E. revolutum rather than E. jurini (reported only from mammals). This is the first report for metacercariae of E. revolutum in the snail host, C. helena, and also confirmed that Filopaludina spp., E. eryresi, and Bithynia spp. act as the second intermediate hosts of E. revolutum under natural conditions, which are indigenously distributed in Chiang Mai province.

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Brief Communication

Prevalence of Haplorchis taichui in Field-Collected Snails: A Molecular Approach
Thapana Chontananarth, Chalobol Wongsawad
Korean J Parasitol 2010;48(4):343-346.
Published online December 16, 2010
DOI: https://doi.org/10.3347/kjp.2010.48.4.343

The prevalence of the cercarial stage of an intestinal trematode, Haplorchis taichui, in thiarid snails (Gastropoda: Thiaridae) was investigated using light microscope and species-specific PCR procedures. A total of 988 snails were collected from Mae Taeng district, Chiang Mai province, northern Thailand, which comprised of 3 species; Melanoides tuberculata, Tarebia granifera, and Thiara scabra. The overall prevalence of pleurolophocercous cercariae was 21.7% as determined by the morphology. For genetic detection of H. taichui infection in snails, 2 primers Hapt_F (5'-GGCCAACGCAATCGTCATCC-3') and Hapt_R (5'-GCGTCGGGTTTCAGACATGG-3'), were used. The genomic DNA of H. taichui, which was used as a positive control, gave an amplification of the 256 bp fragment. The overall prevalence of H. taichui from specific PCR was 9.7%. The proportion of H. taichui among the pleurolophocercous cercariae in this study was 44.9%.

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Original Article

Molecular Markers for Identification of Stellantchasmus falcatus and a Phylogenic Study using the HAT-RAPD Method
Chalobol Wongsawad, Pheravut Wongsawad
Korean J Parasitol 2010;48(4):303-307.
Published online December 16, 2010
DOI: https://doi.org/10.3347/kjp.2010.48.4.303

Stellantchasmus falcatus is a minute intestinal fluke in the family Heterophyidae. Metacercariae, the infective stage, were reported in a marine fish, mullet Liza subviridis, and a fresh water fish, Dermogenus pusillus, in Thailand. Adults were found in chicks, rats, cats, and humans. Morphological studies were done for comparing Stellantchasmus sp. worms found in 2 different fish hosts; their shapes and organ arrangements were very similar except for the prepharynx length. Therefore, the present study aimed to compare their DNA fingerprints using the HAT-RAPD method for both types of Stellantchasmus and several other related species. Ten arbitrarily selected primers (OPA-04, OPA-09, OPN-02, OPN-03, OPN-09, OPN-12, OPP-11, OPR-15, OPX-13, and OPAD-01) were used. It was found that OPA-09, OPN-03, and OPAD-01 were able to generate S. falcatus specific fragments in mullets which consisted of 200, 760, and 280 bp, respectively. In addition, the results of morphologic, DNA fingerprinting, and phylogenetic analyses strongly suggest that the fresh water and marine specimens of Stellantchamus may be different species.

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Brief Communication
Prevalence of Haplorchis taichui and Haplorchoides sp. Metacercariae in Freshwater Fish from Water Reservoirs, Chiang Mai, Thailand
Choosak Nithikathkul, Chalobol Wongsawad
Korean J Parasitol 2008;46(2):109-112.
Published online June 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.2.109

A parasitological investigation on trematode metacercariae was made on 62 freshwater fishes of 13 species in northern Thailand; Cyclocheilichthys apogon, Puntioplites proctozysron, Labiobarbus siamensis, Barbodes gonionotus, Barbodes altus, Henicorhynchus siamensis, Osteochilus hasselti, Notopterus notopterus, Mystacoleucus marginatus, Anabas testudineus, Systomus orphoides, Morulius chrysophykadian, and Hampala macrolepidota. The fish were caught over the summer period (February-May 2007) from 2 Chiang Mai water reservoirs, i.e., the Mae Ngad (UTM 47Q E 503200, 47Q N 2119300) and the Mae Kuang Udomtara (UTM 47Q E 513000, 47Q N 2092600) Reservoirs in Chiang Mai province, Thailand. The prevalence of heterophyid (Haplorchis taichui and Haplorchoides sp.) metacercariae in these fish was 83.9% and 74.2% in the Mae Ngad and Mae Kuang Udomtara Reservoirs, respectively. The highest intensity of heterophyid metacercariae in H. siamensis in the Mae Ngad was 120.4 and that in P. proctozysron in the Mae Kuang Udomtara was 180.0. The fish, A. testudineus, C. apogon, and M. chrysophykadian, were not found to be infected with H. taichui metacercariae. The results show that the freshwater fish in Chiang Mai water reservoirs are heavily infected with H. taichui and Haplorchoides sp. metacercariae.

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PHD : Parasites, Hosts and Diseases