Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.
Semi-Automated Microfluidic Device Combined with a MiniPCR-Duplex Lateral Flow Dipstick for Screening and Visual Species Identification of Lymphatic Filariae Achinya Phuakrod, Navapon Kusuwan, Witsaroot Sripumkhai, Pattaraluck Pattamang, Sirichit Wongkamchai Micromachines.2022; 13(2): 336. CrossRef
Further studies of neuroangiostrongyliasis (rat lungworm disease) in Australian dogs: 92 new cases (2010–2020) and results for a novel, highly sensitive qPCR assay Rogan Lee, Tsung-Yu Pai, Richard Churcher, Sarah Davies, Jody Braddock, Michael Linton, Jane Yu, Erin Bell, Justin Wimpole, Anna Dengate, David Collins, Narelle Brown, George Reppas, Susan Jaensch, Matthew K. Wun, Patricia Martin, William Sears, Jan Šlape Parasitology.2021; 148(2): 178. CrossRef
Sandwich dot-immunogold filtration assay (DIGFA) for specific immunodiagnosis of active neuroangiostrongyliasis Praphathip Eamsobhana, Anchalee Tungtrongchitr, Hoi-Sen Yong, Anchana Prasartvit, Darawan Wanachiwanawin, Xiao-Xian Gan Parasitology.2021; 148(2): 234. CrossRef
Development of a recombinase polymerase amplification (RPA-EXO) and lateral flow assay (RPA-LFA) based on the ITS1 gene for the detection of Angiostrongylus cantonensis in gastropod intermediate hosts Susan I. Jarvi, Elizabeth S. Atkinson, Lisa M. Kaluna, Kirsten A. Snook, Argon Steel Parasitology.2021; 148(2): 251. CrossRef
Genetic Characterization and Detection of Angiostrongylus cantonensis by Molecular Approaches Muxin Chen, Dana Huang, Jiaxu Chen, Yalan Huang, Huiwen Zheng, Yijun Tang, Qian Zhang, Shaohong Chen, Lin Ai, Xiaonong Zhou, Renli Zhang Vector-Borne and Zoonotic Diseases.2021; 21(9): 643. CrossRef
Meningitis patients with Angiostrongylus cantonensis may present without eosinophilia in the cerebrospinal fluid in northern Vietnam Tomoko Hiraoka, Ngo Chi Cuong, Sugihiro Hamaguchi, Mihoko Kikuchi, Shungo Katoh, Le Kim Anh, Nguyen Thi Hien Anh, Dang Duc Anh, Chris Smith, Haruhiko Maruyama, Lay-Myint Yoshida, Do Duy Cuong, Pham Thanh Thuy, Koya Ariyoshi, Alessandra Morassutti PLOS Neglected Tropical Diseases.2020; 14(12): e0008937. CrossRef
Rapid diagnosis of parasitic diseases: current scenario and future needs S. Momčilović, C. Cantacessi, V. Arsić-Arsenijević, D. Otranto, S. Tasić-Otašević Clinical Microbiology and Infection.2019; 25(3): 290. CrossRef
Diagnostic approach to encephalitis and meningoencephalitis in adult returning travellers A. Kenfak, G. Eperon, M. Schibler, F. Lamoth, M.I. Vargas, J.P. Stahl Clinical Microbiology and Infection.2019; 25(4): 415. CrossRef
Survey of Angiostrongylus cantonensis Infection Status in Host Animals and Populations in Shenzhen, 2016–2017 Dana Huang, Yalan Huang, Yijun Tang, Qian Zhang, Xiaoheng Li, Shitong Gao, Wuwei Hua, Renli Zhang Vector-Borne and Zoonotic Diseases.2019; 19(10): 717. CrossRef
Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis Praphathip Eamsobhana, Anchalee Tungtrongchitr, Darawan Wanachiwanawin, Hoi-Sen Yong International Journal of Infectious Diseases.2018; 73: 69. CrossRef
Small-scale spatial analysis of intermediate and definitive hosts of Angiostrongylus cantonensis Qiu-An Hu, Yi Zhang, Yun-Hai Guo, Shan Lv, Shang Xia, He-Xiang Liu, Yuan Fang, Qin Liu, Dan Zhu, Qi-Ming Zhang, Chun-Li Yang, Guang-Yi Lin Infectious Diseases of Poverty.2018;[Epub] CrossRef
Current Trends in Ligand Binding Real-Time Measurement Technologies Stephanie Fraser, Judy Y. Shih, Mark Ware, Edward O’Connor, Mark J. Cameron, Martin Schwickart, Xuemei Zhao, Karin Regnstrom The AAPS Journal.2017; 19(3): 682. CrossRef
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