Parasites, Hosts and Diseases

"Hak-Yong Kim"

Original Article

Evaluation of the Accuracy of the EasyTest™ Malaria Pf/Pan Ag, a Rapid Diagnostic Test, in Uganda: Chom-Kyu Chong, Pyo Yun Cho, Byoung-Kuk Na, Seong Kyu Ahn, Jin Su Kim, Jin-Soo Lee, Sung-Keun Lee, Eun-Taek Han, Hak-Yong Kim, Yun-Kyu Park, Seok Ho Cha, Tong-Soo Kim; Korean J Parasitol 2014;52(5):501-505.
Published online October 22, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.5.501

Abstract
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In recent years, rapid diagnostic tests (RDTs) have been widely used for malaria detection, primarily because of their simple operation, fast results, and straightforward interpretation. The Asan EasyTest™ Malaria Pf/Pan Ag is one of the most commonly used malaria RDTs in several countries, including Korea and India. In this study, we tested the diagnostic performance of this RDT in Uganda to evaluate its usefulness for field diagnosis of malaria in this country. Microscopic and PCR analyses, and the Asan EasyTest™ Malaria Pf/Pan Ag rapid diagnostic test, were performed on blood samples from 185 individuals with suspected malaria in several villages in Uganda. Compared to the microscopic analysis, the sensitivity of the RDT to detect malaria infection was 95.8% and 83.3% for Plasmodium falciparum and non-P. falciparum, respectively. Although the diagnostic sensitivity of the RDT decreased when parasitemia was ≤500 parasites/?l, it showed 96.8% sensitivity (98.4% for P. falciparum and 93.8% for non-P. falciparum) in blood samples with parasitemia ≥100 parasites/?l. The specificity of the RDT was 97.3% for P. falciparum and 97.3% for non-P. falciparum. These results collectively suggest that the accuracy of the Asan EasyTest™ Malaria Pf/Pan Ag makes it an effective point-of-care diagnostic tool for malaria in Uganda.

Citations

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Asymptomatic malaria infection, associated factors and accuracy of diagnostic tests in a historically high transmission setting in Northern Uganda
Bosco B. Agaba, Simon P. Rugera, Ruth Mpirirwe, Martha Atekat, Samuel Okubal, Khalid Masereka, Miseal Erionu, Bosco Adranya, Gertrude Nabirwa, Patrick B. Odong, Yasin Mukiibi, Isaac Ssewanyana, Susan Nabadda, Enoch Muwanguzi
Malaria Journal.2022;[Epub] CrossRef
Genetic diversity of Plasmodium vivax and Plasmodium falciparum lactate dehydrogenases in Myanmar isolates
Jinyoung Lee, Tae Im Kim, Hương Giang Lê, Won Gi Yoo, Jung-Mi Kang, Seong-Kyu Ahn, Moe Kyaw Myint, Khin Lin, Tong-Soo Kim, Byoung-Kuk Na
Malaria Journal.2020;[Epub] CrossRef
Limitations of rapid diagnostic tests in malaria surveys in areas with varied transmission intensity in Uganda 2017-2019: Implications for selection and use of HRP2 RDTs
Agaba B. Bosco, Joaniter I. Nankabirwa, Adoke Yeka, Sam Nsobya, Karryn Gresty, Karen Anderson, Paul Mbaka, Christiane Prosser, David Smith, Jimmy Opigo, Rhoda Namubiru, Emmanuel Arinaitwe, John Kissa, Samuel Gonahasa, Sungho Won, Bora Lee, Chae Seung Lim,
PLOS ONE.2020; 15(12): e0244457. CrossRef
An Update on Malaria Rapid Diagnostic Tests
Avinash N. Mukkala, Jason Kwan, Rachel Lau, David Harris, Dylan Kain, Andrea K. Boggild
Current Infectious Disease Reports.2018;[Epub] CrossRef
Comparative Assessment of Diagnostic Performances of Two Commercial Rapid Diagnostic Test Kits for Detection of Plasmodium spp. in Ugandan Patients with Malaria
Young Yil Bahk, Seo Hye Park, Woojoo Lee, Kyoung Jin, Seong Kyu Ahn, Byoung-Kuk Na, Tong-Soo Kim
The Korean Journal of Parasitology.2018; 56(5): 447. CrossRef
An Evaluation of Active Case Detection in Malaria Control Program in Kiyuni Parish of Kyankwanzi District, Uganda
Young Yil Bahk, Pyo Yun Cho, Seong Kyu Ahn, Woo-Joo Lee, Tong-Soo Kim
The Korean Journal of Parasitology.2018; 56(6): 625. CrossRef
Comparison of the diagnostic performance of microscopic examination with nested polymerase chain reaction for optimum malaria diagnosis in Upper Myanmar
Jung-Mi Kang, Pyo-Yun Cho, Mya Moe, Jinyoung Lee, Hojong Jun, Hyeong-Woo Lee, Seong Kyu Ahn, Tae Im Kim, Jhang Ho Pak, Moe Kyaw Myint, Khin Lin, Tong-Soo Kim, Byoung-Kuk Na
Malaria Journal.2017;[Epub] CrossRef
A Cluster Randomised Trial Introducing Rapid Diagnostic Tests into Registered Drug Shops in Uganda: Impact on Appropriate Treatment of Malaria
Anthony K. Mbonye, Pascal Magnussen, Sham Lal, Kristian S. Hansen, Bonnie Cundill, Clare Chandler, Siân E. Clarke, Roly D Gosling
PLOS ONE.2015; 10(7): e0129545. CrossRef

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Brief Communication

Maintained Seroprevalence of Toxoplasmosis among the Residents of Jeju Island, Korea: Sung-Jong Hong, Chom-Kyu Chong, Kyutaeg Lee, Tong-Soo Kim, Yeon-Pyo Hong, Hye-Jin Ahn, Hak-Yong Kim, A-Ra Ko, Yong-Joo Kim, Ho-Woo Nam; Korean J Parasitol 2011;49(3):309-311.
Published online September 30, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.3.309

Abstract
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Seroepidemiological status of toxoplasmosis among the residents of Jeju island was surveyed and evaluated by ELISA with crude extract of Toxoplasma gondii. The sera of 2,348 residents (male 1,157 and female 1,191) were collected and checked for the IgG antibody titers, which showed 13.2% positive rate (309 sera). The positive rates were increasing gradually according to the age from 4.3% in teenage to 20.6% in seventies. The positive rates were significantly different between the sex by 16.2% for male and 10.2% for female (P<0.05). This positive rate of toxoplasmosis in Jeju island residents is regarded relatively higher than any other regions of Korea. And the high positive rate may be maintained continuously among Jeju island residents without any clear reasons until now but due to some parts peculiar socio-cultural tradition of Jeju island. Therefore, it is necessary to study further the epidemiology of toxoplasmosis of Jeju island.

Citations

Citations to this article as recorded by

Seroprevalence of Toxoplasma gondii assayed using Rapid Diagnostic Tests among Residents in Three Counties Adjacent to The Demilitarized Zone, Korea
Jeehi Jung, Jinyoung Lee, Yoon Kyung Chang, Seong Kyu Ahn, Seo Hye Park, Sung-Jong Hong, Jihoo Lee, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam, Tong-Soo Kim, Dongjae Kim
The Korean Journal of Parasitology.2021; 59(1): 9. CrossRef
Clusters of Toxoplasmosis in Ganghwa-gun, Cheorwon-gun, and Goseong-gun, Korea
Jihye Yu, Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim
The Korean Journal of Parasitology.2021; 59(3): 251. CrossRef
Cross-sectional survey for Toxoplasma gondii infection in humans in Fernando de Noronha island, Brazil
Maria da Conceição Carvalho, Müller Ribeiro-Andrade, Renata Pimentel Bandeira de Melo, Dandara Matias Guedes, José Wilton Pinheiro Junior, Erika Fernanda Torres Samico Fernandes Cavalcanti, Fernando Jorge Rodrigues Magalhães, Rinaldo Aparecido Mota
Revista Brasileira de Parasitologia Veterinária.2021;[Epub] CrossRef
Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea
Yeojin Park, Jinhyeong Noh, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Mi-Sun Yoo, Bo-Ram Yun, Jong-Ho Kim, Eun-Jin Choi, Doo-Sung Cheon, Sung-Jong Hong, Soon-Seek Yoon, Yun Sang Cho
The Korean Journal of Parasitology.2020; 58(3): 257. CrossRef
Clusters of Toxoplasmosis in Gyodong-Myeon and Samsan-Myeon, Ganghwa-Gun, Korea
Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim
The Korean Journal of Parasitology.2020; 58(5): 493. CrossRef
Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets
Won Gi Yoo, Sun-Min Kim, Eun Jeong Won, Ji-Yun Lee, Fuhong Dai, Ho Choon Woo, Ho-Woo Nam, Tae Im Kim, Jeong-Hee Han, Dongmi Kwak, Yun Sang Cho, Seung-Won Kang, Tong-Soo Kim, Xing-Quan Zhu, Chunren Wang, Heejeong Youn, Sung-Jong Hong
The Korean Journal of Parasitology.2018; 56(5): 437. CrossRef
Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea: A Four-Year Follow-up
Yeong Hoon Kim, Ji hoo Lee, Seong kyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
The Korean Journal of Parasitology.2017; 55(3): 247. CrossRef
Seroprevalence of Toxoplasmosis Detected by RDT in Residents near the DMZ (demilitarized zone) of Cheorwon-gun, Gangwon-do, Korea
Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Seongkyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
The Korean Journal of Parasitology.2017; 55(4): 385. CrossRef
Koki Yakushiji
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Mesial Temporal Lobe Epilepsy in Congenital Toxoplasmosis: A Case Report
Woo Kyo Jeong, Byung-Euk Joo, Ji-hye Seo, Jun Kyu Mun, Juhyeon Kim, Dae-Won Seo
Journal of Epilepsy Research.2015; 5(1): 25. CrossRef
Toxoplasma gondii Infection: Seroprevalence and Associated Risk Factors among Primary School Children in the Capital Area of the Republic of the Marshall Islands
Chung-Jung Fu, Ting-Wu Chuang, Huei-Shan Lin, Chih-Hsiung Wu, Yung-Ching Liu, Mailynn K. Langinlur, Min-Yun Lu, Wesley Wei-Wen Hsiao, Chia-Kwung Fan
Japanese Journal of Infectious Diseases.2014; 67(5): 405. CrossRef
A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
Kyoung Ju Song, Zhaoshou Yang, Chom-Kyu Chong, Jin-Soo Kim, Kyung Chan Lee, Tong-Soo Kim, Ho-Woo Nam
The Korean Journal of Parasitology.2013; 51(5): 503. CrossRef
Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea
Hyemi Lim, Sang-Eun Lee, Bong-Kwang Jung, Min-Ki Kim, Mi Youn Lee, Ho-Woo Nam, Jong-Gyun Shin, Cheong-Ha Yun, Han-Ik Cho, Eun-Hee Shin, Jong-Yil Chai
The Korean Journal of Parasitology.2012; 50(4): 287. CrossRef
A Surge in the Seroprevalence of Toxoplasmosis among the Residents of Islands in Gangwha-gun, Incheon, Korea
Zhaoshou Yang, Pyo Yun Cho, Seong Kyu Ahn, Hye-Jin Ahn, Tong-Soo Kim, Chom-Kyu Chong, Sung-Jong Hong, Seok Ho Cha, Ho-Woo Nam
The Korean Journal of Parasitology.2012; 50(3): 191. CrossRef
Seroprevalence of Toxoplasmosis in the Residents of Cheorwon-gun, Gangwon-do, Korea
Hye-Jin Ahn, Pyo Yun Cho, Seong Kyu Ahn, Tong-Soo Kim, Chom-Kyu Chong, Sung-Jong Hong, Seok Ho Cha, Ho-Woo Nam
The Korean Journal of Parasitology.2012; 50(3): 225. CrossRef
Acute Toxoplasmosis Mimicking Melanoma Metastases: Review of Conditions Causing False-Positive Results on ¹⁸F-FDG PET/CT
K. Ivanova, K. Glatz, A. Zippelius, G. Nicolas, P. Itin
Dermatology.2012; 225(4): 349. CrossRef

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Original Article

Development and Clinical Evaluation of a Rapid Serodiagnostic Test for Toxoplasmosis of Cats Using Recombinant SAG1 Antigen: Chom-Kyu Chong, Wooseog Jeong, Hak-Yong Kim, Dong-Jun An, Hye-Young Jeoung, Jeong-Eun Ryu, A-Ra Ko, Yong-Joo Kim, Sung-Jong Hong, Zhaoshou Yang, Ho-Woo Nam; Korean J Parasitol 2011;49(3):207-212.
Published online September 30, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.3.207

Abstract
PDF

Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T. gondii antigens (SAG1, SAG2, GRA3, and GRA6) were produced and tested for the development of rapid diagnostic test (RDT). The proteins were expressed in Escherichia coli, affinity-purified, and applied onto the nitrocellulose membrane of the test strip. The recombinant SAG1 (rSAG1) showed the strongest antigenic activity and highest specificity among them. We also performed clinical evaluation of the rSAG1-loaded RDT in 182 cat sera (55 household and 127 stray cats). The kit showed 0.88 of kappa value comparing with a commercialized ELISA kit, which indicated a significant correlation between rSAG1-loaded RDT and the ELISA kit. The overall sensitivity and specificity of the RDT were 100% (23/23) and 99.4% (158/159), respectively. The rSAG1-loaded RDT is rapid, easy to use, and highly accurate. Thus, it would be a suitable diagnostic tool for rapid detection of antibodies in T. gondii-infected cats under field conditions.

Citations

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