Parasites, Hosts and Diseases

"Ibrahim Rabia"

Original Articles

Monoclonal Antibody-Based Dipstick Assay: A Reliable Field Applicable Technique for Diagnosis of Schistosoma mansoni Infection Using Human Serum and Urine Samples: Zeinab Demerdash, Salwa Mohamed, Mohamed Hendawy, Ibrahim Rabia, Mohy Attia, Zeinab Shaker, Tarek M Diab; Korean J Parasitol 2013;51(1):93-98.
Published online February 18, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.1.93

Abstract
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A field applicable diagnostic technique, the dipstick assay, was evaluated for its sensitivity and specificity in diagnosing human Schistosoma mansoni infection. A monoclonal antibody (mAb) against S. mansoni adult worm tegumental antigen (AWTA) was employed in dipstick and sandwich ELISA for detection of circulating schistosome antigen (CSA) in both serum and urine samples. Based on clinical and parasitological examinations, 60 S. mansoni-infected patients, 30 patients infected with parasites other than schistosomiasis, and 30 uninfected healthy individuals were selected. The sensitivity and specificity of dipstick assay in urine samples were 86.7% and 90.0%, respectively, compared to 90.0% sensitivity and 91.7% specificity of sandwich ELISA. In serum samples, the sensitivity and specificity were 88.3% and 91.7% for dipstick assay vs. 91.7% and 95.0% for sandwich ELISA, respectively. The diagnostic efficacy of dipstick assay in urine and serum samples was 88.3% and 90.0%, while it was 90.8% and 93.3% for sandwich ELISA, respectively. The diagnostic indices of dipstick assay and ELISA either in serum or in urine were statistically comparable (P>0.05). In conclusion, the dipstick assay offers an alternative simple, rapid, non-invasive technique in detecting CSA or complement to stool examinations especially in field studies.

Citations

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Construction of Camelus dromedaries Immune Single Domain Antibodies Library for Development of Schistosoma mansoni Specific Nanobodies Using Phage Display Strategy
Hadeer Adel El-Kalamawy, Mohammed H. Awwad, Tarek M. Diab, Hend Okasha, Amal M. Abdel-Kareim, Marawan A. Marawan, Salma A. Shoulah, Ehab El-Dabaa
Recent Patents on Biotechnology.2025; 19(1): 69. CrossRef
Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases
Madeleine J. Rogers, Donald P. McManus, Stephen Muhi, Catherine A. Gordon
Clinical Microbiology Reviews.2021;[Epub] CrossRef
Serum low replacement medium versus serum rich replacement medium for production of Anti-Schistosoma Monoclonal antibody: A comparative study
Faten S. Mahmoud, Zeinab A. Demerdash, Sherihan M. Elmotawakel, Salwa Hassan, Mohamed Hendawy, Shimaa A. Atta, Shereen M. Shawky, Engy M. Alkhateeb, Hanaa I. Hassanin
Clinical Epidemiology and Global Health.2020; 8(2): 423. CrossRef
A protease-based biosensor for the detection of schistosome cercariae
A. J. Webb, R. Kelwick, M. J. Doenhoff, N. Kylilis, J. T. MacDonald, K. Y. Wen, C. McKeown, G. Baldwin, T. Ellis, K. Jensen, P. S. Freemont
Scientific Reports.2016;[Epub] CrossRef
Diagnostic Tests to Support Late-Stage Control Programs for Schistosomiasis and Soil-Transmitted Helminthiases
Kenneth R. Hawkins, Jason L. Cantera, Helen L. Storey, Brandon T. Leader, Tala de los Santos, Justin V. Remais
PLOS Neglected Tropical Diseases.2016; 10(12): e0004985. CrossRef
Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil
Maria Cristina Carvalho Espírito-Santo, Mónica Viviana Alvarado-Mora, Pedro Luiz Silva Pinto, Maria Carmen Arroyo Sanchez, Emmanuel Dias-Neto, Vera Lúcia Pagliusi Castilho, Elenice Messias do Nascimento Gonçalves, Pedro Paulo Chieffi, Expedito José de Alb
BioMed Research International.2015; 2015: 1. CrossRef
Diagnosis of Parasitic Infections: What’s Going On?
Alessandra Ricciardi, Momar Ndao
SLAS Discovery.2015; 20(1): 6. CrossRef

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Protective Role of Purified Cysteine Proteinases against Fasciola gigantica Infection in Experimental Animals: Eman EL-Ahwany, Ibrahim Rabia, Faten Nagy, Mona Zoheiry, Tarek Diab, Suher Zada; Korean J Parasitol 2012;50(1):45-51.
Published online March 6, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.1.45

Abstract
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Fascioliasis is one of the public health problems in the world. Cysteine proteinases (CP) released by Fasciola gigantica play a key role in parasite feeding, migration through host tissues, and in immune evasion. There has been some evidence from several parasite systems that proteinases might have potential as protective antigens against parasitic infections. Cysteine proteinases were purified and tested in vaccine trials of sheep infected with the liver fluke. Multiple doses (2 mg of CP in Freund's adjuvant followed by 3 booster doses 1 mg each at 4 week intervals) were injected intramuscularly into sheep 1 week prior to infect orally with 300 F. gigantica metacercariae. All the sheep were humanely slaughtered 12 weeks after the first immunization. Changes in the worm burden, ova count, and humoral and cellular responses were evaluated. Significant reduction was observed in the worm burden (56.9%), bile egg count (70.7%), and fecel egg count (75.2%). Immunization with CP was also found to be associated with increases of total IgG, IgG₁, and IgG₂ (P<0.05). Data showed that the serum cytokine levels of pro-inflammatory cytokines, IL-12, IFN-γ, and TNF-α, revealed significant decreases (P<0.05). However, the anti-inflammatory cytokine levels, IL-10, TGF-β, and IL-6, showed significant increases (P<0.05). In conclusion, it has been found that CP released by F. gigantica are highly important candidates for a vaccine antigen because of their role in the fluke biology and host-parasite relationships.

Citations

Citations to this article as recorded by

Genetic diversity and adaptability of native sheep breeds from different climatic zones
George Wanjala, Zoltán Bagi, Dinu Gavojdian, Bouabid Badaoui, Putri Kusuma Astuti, Alexandru Mizeranschi, Elena Ilisiu, Husein Ohran, Eva Pasic Juhas, Dimitrios Loukovitis, Aldona Kawęcka, Rūta Šveistienė, Zsolt Becskei, Péter Strausz, Nelly Kichamu, Szil
Scientific Reports.2025;[Epub] CrossRef
Serodiagnosis of Fasciola gigantica Infection in Buffaloes with Native Cathepsin-L Proteases and Recombinant Cathepsin L1-D
Andleeb Aftab, Rohit Lall, Savita Bisen, Arun Anandanarayanan, Ajayta Rialch, Jayanta K. Chamuah, Shobha Yadav, M. Silamparasan, O. K. Raina
Acta Parasitologica.2020; 65(2): 413. CrossRef
A Survey on the Adjuvant Role of Naloxone Alone or Combined with Alum in Vaccination Against Fasciolosis in BALB/c Mice
Hakim Azizi, Hadi Mirzaeei, Amin Bagheri, Ali Bazi, Ali Khamesipour, Hajar Yaghoobi, Aliyar Mirzapour, Mehrdad Khatami, Samira Elikaee
Acta Parasitologica.2019;[Epub] CrossRef
Oral delivery of Bacillus subtilis spores expressing cysteine protease of Clonorchis sinensis to grass carp ( Ctenopharyngodon idellus ): Induces immune responses and has no damage on liver and intestine function
Zeli Tang, Hengchang Sun, TingJin Chen, Zhipeng Lin, Hongye Jiang, Xinyi Zhou, Cunbin Shi, Houjun Pan, Ouqin Chang, Pengli Ren, Jinyun Yu, Xuerong Li, Jin Xu, Yan Huang, Xinbing Yu
Fish & Shellfish Immunology.2017; 64: 287. CrossRef
Comparative analysis of immune effects in mice model: Clonorchis sinensis cysteine protease generated from recombinant Escherichia coli and Bacillus subtilis spores
Zhanshuai Wu, Zeli Tang, Mei Shang, Lu Zhao, Lina Zhou, Xiangzhan Kong, Zhipeng Lin, Hengchang Sun, Tingjin Chen, Jin Xu, Xuerong Li, Yan Huang, Xinbing Yu
Parasitology Research.2017; 116(7): 1811. CrossRef
The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease
Zeli Tang, Mei Shang, Tingjin Chen, Pengli Ren, Hengchang Sun, Hongling Qu, Zhipeng Lin, Lina Zhou, Jinyun Yu, Hongye Jiang, Xinyi Zhou, Xuerong Li, Yan Huang, Jin Xu, Xinbing Yu
Parasites & Vectors.2016;[Epub] CrossRef
Immunological features of LPS from Ochrobactrum intermedium on sheep experimentally infected with Fasciola hepatica
J.M. Martínez-Pérez, D. Robles-Pérez, F.A. Rojo-Vázquez, M. Martínez-Valladares
Research in Veterinary Science.2014; 97(2): 329. CrossRef
Liver fluke vaccines in ruminants: strategies, progress and future opportunities
Hayley Toet, David M. Piedrafita, Terry W. Spithill
International Journal for Parasitology.2014; 44(12): 915. CrossRef

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Fasciola gigantica Fatty Acid Binding Protein (FABP) as a Prophylactic Agent against Schistosoma mansoni Infection in CD1 Mice: Ibrahim Rabia Aly, M. Diab, A. M. El-Amir, M. Hendawy, S. Kadry; Korean J Parasitol 2012;50(1):37-43.
Published online March 6, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.1.37

Abstract
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Although schistosomicidal drugs and other control measures exist, the advent of an efficacious vaccine remains the most potentially powerful means for controlling this disease. In this study, native fatty acid binding protein (FABP) from Fasciola gigantica was purified from the adult worm's crude extract by saturation with ammonium sulphate followed by separation on DEAE-Sephadex A-50 anion exchange chromatography and gel filtration using Sephacryl HR-100, respectively. CD1 mice were immunized with the purified, native F. gigantica FABP in Freund's adjuvant and challenged subcutaneously with 120 Schistosoma mansoni cercariae. Immunization of CD1 mice with F. gigantica FABP has induced heterologous protection against S. mansoni, evidenced by the significant reduction in mean worm burden (72.3%), liver and intestinal egg counts (81.3% and 80.8%, respectively), and hepatic granuloma counts (42%). Also, it elicited mixed IgG₁/IgG_2b immune responses with predominant IgG1 isotype, suggesting that native F. gigantica FABP is mediated by a mixed Th1/Th2 response. However, it failed to induce any significant differences in the oogram pattern or in the mean granuloma diameter. This indicated that native F. gigantica FABP could be a promising vaccine candidate against S. mansoni infection.

Citations

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Investigating the Taenia solium Fatty Acid Binding Protein Superfamily for Their Immunological Outlook and Prospect for Therapeutic Targets
Suraj S. Rawat, Gagandeep Singh, Amit Prasad
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Bartholomew N. Ondigo, Rachael E. Hamilton, Edwin O. Magomere, Isaac O. Onkanga, Pauline N. Mwinzi, Maurice R. Odiere, Lisa Ganley-Leal
Frontiers in Immunology.2022;[Epub] CrossRef
Effect of regulatory T cells on the efficacy of the fatty acid-binding protein vaccine against Schistosoma japonicum
Chun-lian Tang, Rong-hui Zhang, Zhi-ming Liu, Huang Jin, Long He
Parasitology Research.2019; 118(2): 559. CrossRef
Protection against Schistosoma mansoni infection using a Fasciola hepatica-derived fatty acid binding protein from different delivery systems
Belén Vicente, Julio López-Abán, Jose Rojas-Caraballo, Esther del Olmo, Pedro Fernández-Soto, Antonio Muro
Parasites & Vectors.2016;[Epub] CrossRef
Schistosomiasis in Egypt: A never-ending story?
Ahmad A. Othman, Rasha H. Soliman
Acta Tropica.2015; 148: 179. CrossRef
Development of the Brazilian Anti Schistosomiasis Vaccine Based on the Recombinant Fatty Acid Binding Protein Sm14 Plus GLA-SE Adjuvant
Miriam Tendler, Marilia Almeida, Andrew Simpson
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The transcriptome of Echinostoma caproni adults: Further characterization of the secretome and identification of new potential drug targets
Gagan Garg, Dolores Bernal, Maria Trelis, Javier Forment, Javier Ortiz, M. Luz Valero, Laia Pedrola, Juan Martinez-Blanch, J. Guillermo Esteban, Shoba Ranganathan, Rafael Toledo, Antonio Marcilla
Journal of Proteomics.2013; 89: 202. CrossRef
Molecular characterization, functional expression, tissue localization and protective potential of a Taenia solium fatty acid-binding protein
Oscar Illescas, Julio C. Carrero, Raúl J. Bobes, Ana Flisser, Gabriela Rosas, Juan P. Laclette
Molecular and Biochemical Parasitology.2012; 186(2): 117. CrossRef

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T Regulatory Cell Responses to Immunization with a Soluble Egg Antigen in Schistosoma mansoni-Infected Mice: Eman El-Ahwany, Ibrahim Rabia Bauiomy, Faten Nagy, Rabab Zalat, Ola Mahmoud, Suher Zada; Korean J Parasitol 2012;50(1):29-35.
Published online March 6, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.1.29

Abstract
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The aim of the study is to characterize the phenotypes of CD4⁺ CD25⁺ T regulatory cells within the liver granulomas and association with both Foxp-3 gene expression and splenic cytokines. Na?ve C57BL/6 mice were intravenously injected with multiple doses of the soluble egg antigen (SEA) 7 days before cercarial infection. The immunized and infected control groups were sacrificed 8 and 16 weeks post-infection (PI). Histopathology, parasitological parameters, splenic phenotypes for T regulatory cells, the FOXP-3 expression in hepatic granuloma using real-time PCR, and the associated splenic cytokines were studied. Histopathological examination of the liver revealed remarkable increase in degenerated ova within hepatic granuloma which decreased in diameter at weeks 8 and 16 PI (P<0.01). The percentage of T regulatory cells (CD4⁺ CD25⁺) increased significantly (P<0.01) in the immunized group compared to the infected control at weeks 8 and 16 PI. The FOXP-3 expression in hepatic granulomas increased from 10 at week 8 to 30 fold at week 16 PI in the infected control group. However, its expression in the immunized group showed an increase from 30 at week 8 to 70 fold at week 16 PI. The splenic cytokine levels of pro-inflammatory cytokines, IFN-γ, IL-4, and TNF-α, showed significant decreases (P<0.05) compared to the infected control group. In conclusion, the magnitude and phenotype of the egg-induced effects on T helper responses were found to be controlled by a parallel response within the T regulatory population which provides protection in worm parasite-induced immunopathology.

Citations

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