Parasites, Hosts and Diseases

"Jae-Hwan Kim"

Original Article

Diagnostic Efficacy of a Recombinant Cysteine Protease of Spirometra erinacei Larvae for Serodiagnosis of Sparganosis: S.M. Mazidur Rahman, Jae-Hwan Kim, Sung-Tae Hong, Min-Ho Choi; Korean J Parasitol 2014;52(1):41-46.
Published online February 19, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.1.41

Abstract
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The mature domain of a cysteine protease of Spirometra erinacei plerocercoid larva (i.e., sparganum) was expressed in Escherichia coli, and its value as an antigen for the serodiagnosis of sparganosis was investigated. The recombinant protein (rSepCp-1) has the molecular weight of 23.4 kDa, and strongly reacted with the sparganum positive human or mice sera but not with negative sera by immunoblotting. ELISA with rSepCp-1 protein or sparganum crude antigen (SeC) was evaluated for the serodiagnosis of sparganosis using patient's sera. The sensitivity and specificity of ELISA using rSepCp-1 protein were 95.0% (19/20) and 99.1% (111/112), respectively. In contrast, the sensitivity and specificity of ELISA with SeC were 100% (20/20) and 96.4% (108/112), respectively. Moreover, in experimentally infected mice, the sensitivity and specificity of both ELISA assays were 100% for the detection of anti-sparganum IgG. It is suggested that the rSepCp-1 protein-based ELISA could provide a highly sensitive and specific assay for the diagnosis of sparganosis.

Citations

Citations to this article as recorded by

Development of an immunochromatographic test for serodiagnosis of human sparganosis
Jitaporn Harasan, Lakkhana Sadaow, Patcharaporn Boonroumkaew, Rutchanee Rodpai, Oranuch Sanpool, Hiroshi Yamasaki, Pewpan M. Intapan, Wanchai Maleewong
Parasitology Research.2025;[Epub] CrossRef
Establishment of Animal Infection Model of Spirometra Mansoni and Identification of Spirometra Mansoni by Enzyme-Linked Immunosorbent Assay
Anqi Luo, Shuyu Chen, Mingye He, Xiaoruo Tan, Zhikang Li, Wei Liu, Yisong Liu
Vector-Borne and Zoonotic Diseases.2024;[Epub] CrossRef
Case Report: Moving Tumor-Like Foci Behind Refractory Epilepsy-Cerebral Sparganosis Successfully Treated by Surgery After Failure of Praziquantel Treatment
Yusi Chen, Xu Chen, Huicong Kang
Frontiers in Neurology.2022;[Epub] CrossRef
Standardization of recombinant Ancylostoma caninum cysteine protease 2 (rAcCP2) based indirect ELISA for serodiagnosis of hookworm infection in dogs
SUCHITA P UKE, RAJAT GARG, SHAFIYA IMTIAZ RAFIQI, HIRA RAM, K L KHURANA, P S BANERJEE
The Indian Journal of Animal Sciences.2018; 88(2): 153. CrossRef
Molecular Identification of Spirometra erinaceieuropaei Tapeworm in Cases of Human Sparganosis, Hong Kong
Tommy H.C. Tang, Samson S.Y. Wong, Christopher K.C. Lai, Rosana W.S. Poon, Helen S.Y. Chan, Tak Chiu Wu, Yuk-Fai Cheung, Tak-Lap Poon, Yi-Po Tsang, Wai-Lun Tang, Alan K.L. Wu
Emerging Infectious Diseases.2017; 23(4): 665. CrossRef
Serodiagnosis of sparganosis by ELISA using recombinant cysteine protease of Spirometra erinaceieuropaei spargana
Li Na Liu, Xi Zhang, Peng Jiang, Ruo Dan Liu, Jian Zhou, Rui Zhe He, Jing Cui, Zhong Quan Wang
Parasitology Research.2015; 114(2): 753. CrossRef
Human sparganosis, a neglected food borne zoonosis
Quan Liu, Ming-Wei Li, Ze-Dong Wang, Guang-Hui Zhao, Xing-Quan Zhu
The Lancet Infectious Diseases.2015; 15(10): 1226. CrossRef
Clinical Features of Pulmonary Sparganosis
Ning Li, Yi Xiang, Yun Feng, Min Li, Bei Li Gao, Qing Yun Li
The American Journal of the Medical Sciences.2015; 350(6): 436. CrossRef
Characterization of Spirometra erinaceieuropaei Plerocercoid Cysteine Protease and Potential Application for Serodiagnosis of Sparganosis
Li Na Liu, Zhong Quan Wang, Xi Zhang, Peng Jiang, Xin Qi, Ruo Dan Liu, Zi Fang Zhang, Jing Cui, Xiao-Nong Zhou
PLOS Neglected Tropical Diseases.2015; 9(6): e0003807. CrossRef
Molecular characterization of a Spirometra mansoni antigenic polypeptide gene encoding a 28.7 kDa protein
Jing Cui, Tong Wei, Li Na Liu, Xi Zhang, Xin Qi, Zi Fang Zhang, Zhong Quan Wang
Parasitology Research.2014; 113(9): 3511. CrossRef
Development of a Rapid Diagnostic Kit That Uses an Immunochromatographic Device To Detect Antibodies in Human Sparganosis
Hiroshi Yamasaki, Takeshi Nakamura, Pewpan M. Intapan, Wanchai Maleewong, Yasuyuki Morishima, Hiromu Sugiyama, Hiroyuki Matsuoka, Kaoru Kobayashi, Katsuyoshi Takayama, Yukuharu Kobayashi, P. P. Wilkins
Clinical and Vaccine Immunology.2014; 21(9): 1360. CrossRef

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Brief Communication

Collection of Clonorchis sinensis adult worms from infected humans after praziquantel treatment: Chenghua Shen, Jae-hwan Kim, Jeong-Keun Lee, Young Mee Bae, Min-Ho Choi, Jin-Kyoung Oh, Min Kyung Lim, Hai-Rim Shin, Sung-Tae Hong; Korean J Parasitol 2007;45(2):149-152.
Published online June 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.2.149

Abstract
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A cohort was established for evaluation of cancer risk factors in Sancheong-gun, Gyeongsangnam-do, Korea. As one of the cohort studies, stools of 947 residents (403 males and 544 females, age range: 29-86 years) were screened for Clonorchis sinensis eggs using both Kato-Katz method and formalin-ether sedimentation technique. The overall egg positive rate of C. sinensis was 37.7% and individual EPG (eggs per gram of feces) counts ranged from 24 to 28,800. Eight egg positive residents voluntarily joined a process of collection of the passed worms after praziquantel treatment. A total of 158 worms were recovered from 5 of the 8 treated persons, ranged from 3 to 108 in each individual. The worms were 15-20 mm × 2-3 mm in size, and showed brown-pigmented, red, or white body colors. This is the first collection record of C. sinensis adult worms from humans through anthelmintic treatment and purgation. The adult worms of C. sinensis may be paralyzed by praziquantel and then discharged passively through bile flow in the bile duct and by peristaltic movement of the bowel.

Citations

Citations to this article as recorded by

Clonorchis sinensis and Cholangiocarcinoma
Eun-Min Kim, Sung-Tae Hong
Journal of Korean Medical Science.2025;[Epub] CrossRef
Natural history of liver fluke infection underpins epidemiological patterns of biliary cancer
Thomas Crellen, Francesca Vita, Chiara Braconi, Paiboon Sithithaworn, T. Déirdre Hollingsworth
Proceedings of the National Academy of Sciences.2025;[Epub] CrossRef
Diagnosis of helminths depends on worm fecundity and the distribution of parasites within hosts
Thomas Crellen, Melissa Haswell, Paiboon Sithithaworn, Somphou Sayasone, Peter Odermatt, Poppy H. L. Lamberton, Simon E. F. Spencer, T. Déirdre Hollingsworth
Proceedings of the Royal Society B: Biological Sciences.2023;[Epub] CrossRef
A comparison of helminth infections as assessed through coprological analysis and adult worm burdens in a wild host
Rachel L. Byrne, Ursula Fogarty, Andrew Mooney, Nicola M. Marples, Celia V. Holland
International Journal for Parasitology: Parasites and Wildlife.2018; 7(3): 439. CrossRef
Application of a loop-mediated isothermal amplification (LAMP) assay targeting cox1 gene for the detection of Clonorchis sinensis in human fecal samples
S. M. Mazidur Rahman, Hyun Beom Song, Yan Jin, Jin-Kyoung Oh, Min Kyung Lim, Sung-Tae Hong, Min-Ho Choi, Justin V. Remais
PLOS Neglected Tropical Diseases.2017; 11(10): e0005995. CrossRef
Cohort Profile: Community-based prospective cohort from the National Cancer Center, Korea
Jin-Kyoung Oh, Min Kyung Lim, E Hwa Yun, Min-Ho Choi, Sung-Tae Hong, Soung-Hoon Chang, Sue Kyung Park, Sung-Il Cho, Dong-Hyun Kim, Keun-Young Yoo, Hai-Rim Shin
International Journal of Epidemiology.2015; : dyv302. CrossRef
An EF-handed Ca2+-binding protein of Chinese liver fluke Clonorchis sinensis
Eun Joo Chung, Tae Yun Kim, Sung-Jong Hong, Tai-Soon Yong
Parasitology Research.2013; 112(12): 4121. CrossRef
Clonorchis sinensis and clonorchiasis, an update
Sung-Tae Hong, Yueyi Fang
Parasitology International.2012; 61(1): 17. CrossRef
Coproantigen capture ELISA for detection of Clonorchis sinensis infection in experimentally infected rats
S.M. Mazidur Rahman, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong
Parasitology International.2012; 61(1): 203. CrossRef
Oriental Cholangiohepatitis (Clonorchiasis Infestation) Caused by Clonorchis Sinensis
Sarah Klemencic, Michael Phelan, Robert Patrick, Noushin Vahdat
The Journal of Emergency Medicine.2012; 43(2): e107. CrossRef
Early detection and estimation of infection burden by real-time PCR in rats experimentally infected with Clonorchis sinensis
S. M. Mazidur Rahman, Young Mee Bae, Sung-Tae Hong, Min-Ho Choi
Parasitology Research.2011; 109(2): 297. CrossRef
Hypersensitive Reaction to Praziquantel in a Clonorchiasis Patient
Jung-Min Lee, Hyun-Sul Lim, Sung-Tae Hong
The Korean Journal of Parasitology.2011; 49(3): 273. CrossRef
Obstructive Jaundice Caused by Clonorchiasis-associated Duodenal Papillitis: A Case Report
Jun Uk Lim, Kwang Ro Joo, Hyun Phil Shin, Jae Myung Cha, Joung Il Lee, Sung Jig Lim
Journal of Korean Medical Science.2011; 26(1): 135. CrossRef
Correlation between Discharged Worms and Fecal Egg Counts in Human Clonorchiasis
Jae-Hwan Kim, Min-Ho Choi, Young Mee Bae, Jin-Kyoung Oh, Min Kyung Lim, Sung-Tae Hong, Akira Ito
PLoS Neglected Tropical Diseases.2011; 5(10): e1339. CrossRef