This is the first study to report Chrysomya pinguis (Walker) and Lucilia porphyrina (Walker) (Diptera: Calliphoridae) as forensically important blow fly species from human cadavers in Thailand, in addition to Chrysomya villeneuvi (Patton) already known in Thailand. In 2016, a fully decomposed body of an unknown adult male was discovered in a high mountainous forest during winter in Chiang Mai province. The remains were infested heavily with thousands of blow fly larvae feeding simultaneously on them. Morphological identification of adults reared from the larvae, and molecular analysis based on sequencing of 1,247 bp partial mitochondrial cytochrome c oxidase subunit 1 gene (CO1) of the larvae and puparia, confirmed the above mentioned 3 species. The approving forensic fly evidence by molecular approach was described for the first time in Thailand. Moreover, neighbor-joining phylogenetic analysis of the CO1 was performed to compare the relatedness of the species, thereby affirming the accuracy of identification. As species of entomofauna varies among cases in different geographic and climatic circumstances, C. pinguis and L. porphyrina were added to the list of Thai forensic entomology caseworks, including colonizers of human remains in open, high mountainous areas during winter. Further research should focus on these 3 species, for which no developmental data are currently available.
Citations
Citations to this article as recorded by
Origins and Diversification of Myiasis Across Blowflies Gisele Antoniazzi Cardoso, Vanessa A. S. Cunha, Bruno C. Genevcius, Tais Madeira‐Ott, Bárbara Maria de Andrade Costa, Daniela Munhoz Rossoni, Patricia Jacqueline Thyssen, Tatiana Teixeira Torres Ecology and Evolution.2025;[Epub] CrossRef
Carrion‐breeding flies of Australia and New Zealand: A review and key to adults Nikolas P. Johnston, Nathan J. Butterworth, Kelly A. Meiklejohn, Andrzej Grzywacz, Thomas Pape, Krzysztof Szpila, James F. Wallman Austral Entomology.2025;[Epub] CrossRef
Initial insights into using outline-based geometric morphometrics of the larval cephaloskeleton to identify forensically important calliphorids in Thailand Wanida Kanta, Sangob Sanit, Kabkaew L Sukontason, Jean-Pierre Dujardin, Sebastien Dujardin, Kwankamol Limsopatham, Ian Dadour Journal of Medical Entomology.2025;[Epub] CrossRef
Geometry of posterior larval spiracles to identify medically and forensically important calliphorids in Thailand Wanida Kanta, Kwankamol Limsopatham, Kabkaew L. Sukontason, Kom Sukontason, Jean-Pierre Dujardin, Sebastien Dujardin, Sangob Sanit Acta Tropica.2024; 252: 107126. CrossRef
The Study of Forensically Important Insects Recovered from Human Corpses in Taiwan Wei-Lun Yan, Chiou-Herr Yang, Siew Hwa Tan, Chung-Yen Pai, Kan-Kun Li, Chen-Chou Chung Insects.2023; 14(4): 346. CrossRef
Wing morphometrics as a tool for the identification of forensic important Lucilia spp. (Diptera: Calliphoridae) Kwankamol Limsopatham, Chatnaphat Tanajitaree, Sangob Sanit, Kom Sukontason, Pradya Somboon, Jens Amendt, Nina Feddern, Kabkaew L. Sukontason Acta Tropica.2022; 226: 106242. CrossRef
Mitogenome-wise codon usage pattern from comparative analysis of the first mitogenome of Blepharipa sp. (Muga uzifly) with other Oestroid flies Debajyoti Kabiraj, Hasnahana Chetia, Adhiraj Nath, Pragya Sharma, Ponnala Vimal Mosahari, Deepika Singh, Palash Dutta, Kartik Neog, Utpal Bora Scientific Reports.2022;[Epub] CrossRef
Chrysomya pinguis (Walker) (Diptera: Calliphoridae), blow fly of forensic importance: A review of bionomics and forensic entomology appraisal Kabkaew L. Sukontason, Sangob Sanit, Kwankamol Limsopatham, Anchalee Wannasan, Pradya Somboon, Kom Sukontason Acta Tropica.2022; 232: 106506. CrossRef
ORIGIN OF MYIASIS Alen J Salerian INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH.2022; : 39. CrossRef
Stay cool or get hot? An applied primer for using temperature in forensic entomological case work Lena Lutz, Jens Amendt Science & Justice.2020; 60(5): 415. CrossRef
Review of Molecular Identification Techniques for Forensically Important Diptera M Denise Gemmellaro, George C Hamilton, Jessica L Ware Journal of Medical Entomology.2019; 56(4): 887. CrossRef
Gene expression during the intra-puparial stage of Chrysomya megacephala: Implications for postmortem interval estimation Yu Wang, Zhiya Gu, Liangliang Li, Jiangfeng Wang Journal of Asia-Pacific Entomology.2019; 22(3): 841. CrossRef
Temperature-dependent development of the blow fly
Chrysomya pinguis
and its significance in estimating postmortem interval
Yingna Zhang, Yu Wang, Jian Sun, Guoliang Hu, Man Wang, Jens Amendt, Jiangfeng Wang Royal Society Open Science.2019; 6(9): 190003. CrossRef
Chrysomya chani Kurahashi (Diptera: Calliphoridae), a blow fly species of forensic importance: morphological characters of the third larval instar and a case report from Thailand Kabkaew L. Sukontason, Tanin Bhoopat, Anchalee Wannasan, Narin Sontigun, Sangob Sanit, Jens Amendt, Chutharat Samerjai, Kom Sukontason Forensic Sciences Research.2018; 3(1): 83. CrossRef
Predicting Geographic Distribution of Forensically Significant Blow Flies of Subfamily Chrysomyinae (Diptera: Calliphoridae) in Northern Thailand Tunwadee Klong-klaew, Ratchadawan Ngoen-klan, Kittikhun Moophayak, Kom Sukontason, Kim N. Irvine, Jeffery K. Tomberlin, Pradya Somboon, Theeraphap Chareonviriyaphap, Hiromu Kurahashi, Kabkaew L. Sukontason Insects.2018; 9(3): 106. CrossRef
Molecular Analysis of Forensically Important Blow Flies in Thailand Narin Sontigun, Kabkaew L. Sukontason, Jens Amendt, Barbara K. Zajac, Richard Zehner, Kom Sukontason, Theeraphap Chareonviriyaphap, Anchalee Wannasan Insects.2018; 9(4): 159. CrossRef
Spatial Distribution of Forensically Significant Blow Flies in Subfamily Luciliinae (Diptera: Calliphoridae), Chiang Mai Province, Northern Thailand: Observations and Modeling Using GIS Tunwadee Klong-klaew, Ratchadawan Ngoen-klan, Kittikhun Moophayak, Kom Sukontason, Kim N. Irvine, Jeffery K. Tomberlin, Hiromu Kurahashi, Theeraphap Chareonviriyaphap, Pradya Somboon, Kabkaew L. Sukontason Insects.2018; 9(4): 181. CrossRef
Morphology of immature stages of blow fly, Lucilia sinensis Aubertin (Diptera: Calliphoridae), a potential species of forensic importance Sangob Sanit, Kom Sukontason, Hiromu Kurahashi, Jeffery K. Tomberlin, Anchalee Wannasan, Rungroj Kraisittipanit, Kabkaew L. Sukontason Acta Tropica.2017; 176: 395. CrossRef
This study aimed to investigate the morphology and reconstruct the phylogenetic relationships of Centrocestus formosanus originating from 5 species of freshwater fish, i.e., Esomus metallicus, Puntius brevis, Anabas testudineus, Parambassis siamensis, and Carassius auratus, in Chiang Mai province, Thailand. Sequence-related amplified polymorphism (SRAP) and phylogeny based on internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (CO1) were performed. The results showed similar morphologies of adult C. formosanus from day 5 after infection in chicks. C. formosanus originated from 4 species of freshwater fish had the same number of circumoral spines on the oral sucker, except for those from C. auratus which revealed 34 circumoral spines. The phylogenetic tree obtained from SRAP profile and the combination of ITS2 and CO1 sequence showed similar results that were correlated with the number of circumoral spines in adult worms. Genetic variability of C. formosanus also occurred in different species of freshwater fish hosts. However, more details of adult worm morphologies and more sensitive genetic markers are needed to confirm the species validity of C. formosanus with 34 circumoral spines originating from C. auratus in the future.
Citations
Citations to this article as recorded by
Loop‐Mediated Isothermal Amplification Combined With Lateral‐Flow Dipstick for Detection of Centrocestus formosanus in Ornamental Fish Metawee Sabaijai, Thanawan Tejangkura, Thapana Chontananarth Journal of Fish Diseases.2025;[Epub] CrossRef
Revision of Cryptogonimus Osborn, 1903 and Caecincola Marshall et Gilbert, 1905 (Digenea: Cryptogonimidae), supplemental description of Cryptogonimus chili Osborn, 1903, and description of a new species of Caecincola infecting basses (Centrarchiformes: Ce Triet N. Truong, Stephen S. Curran, Brandon L. Simcox, Alex P. Bybel, Stephen A. Bullard Folia Parasitologica.2025;[Epub] CrossRef
Innovative molecular and immunological approaches of heterophyiasis infecting some Egyptian marketed fishes Olfat A. Mahdy, Reem M. Ramadan, Mai A. Salem BMC Veterinary Research.2024;[Epub] CrossRef
Multiplex PCR development for the simultaneous and rapid detection of two pathogenic flukes, Dactylogyrus spp. and Centrocestus formosanus, in ornamental fishes Phonkawin Jaruboonyakorn, Thanawan Tejangkura, Thapana Chontananarth Aquaculture.2022; 548: 737660. CrossRef
Efficacy of Acriflavine for controlling parasitic diseases in farmed Nile tilapia with emphasis on fish health, gene expression analysis, oxidative stress, and histopathological alterations Mahmoud Abou-Okada, Huda O. AbuBakr, Azza Hassan, Shimaa Abdel-Radi, Samira H. Aljuaydi, Mohamed Abdelsalam, Engy Taha, Nehal A. Younis, Dalia A. Abdel-Moneam Aquaculture.2021; 541: 736791. CrossRef
A review of molecular identification tools for the opisthorchioidea Maureen Duflot, Thomas Setbon, Graziella Midelet, Thomas Brauge, Mélanie Gay Journal of Microbiological Methods.2021; 187: 106258. CrossRef
Streptococcus, Centrocestus formosanus and Myxobolus tilapiae concurrent infections in farmed Nile tilapia (Oreochromis niloticus) Alaa Eldin Eissa, Marwa M. Attia, Mamdouh Y. Elgendy, Gehad A. Ismail, Nader M. Sabry, Abdelbary Prince, Mahmoud A. Mahmoud, Ghada O. El-Demerdash, Mohamed Abdelsalam, Hassan I.M. Derwa Microbial Pathogenesis.2021; 158: 105084. CrossRef
Molecular classification of rumen fluke eggs in fecal specimens from Suphanburi Province, Thailand, based on cytochrome C oxidase subunit 1 Sothorn Anucherngchai, Thapana Chontananarth, Thanawan Tejangkura, Chalobol Wongsawad Veterinary Parasitology: Regional Studies and Reports.2020; 20: 100382. CrossRef
Infections of Digenetic Trematode Metacercariae in Wrestling Halfbeak, Dermogenys pusilla from Bangkok Metropolitan Region in Thailand Laddawan Patarwut, Thapana Chontananarth, Jong-Yil Chai, Watchariya Purivirojkul The Korean Journal of Parasitology.2020; 58(1): 27. CrossRef
Diagnosis of Centrocestus formosanus Infection in Zebrafish (Danio rerio) in Italy: A Window to a New Globalization-Derived Invasive Microorganism Antonino Pace, Ludovico Dipineto, Serena Aceto, Maria Concetta Censullo, Maria Carmen Valoroso, Lorena Varriale, Laura Rinaldi, Lucia Francesca Menna, Alessandro Fioretti, Luca Borrelli Animals.2020; 10(3): 456. CrossRef
The life cycle of a zoonotic parasite reassessed: Experimental infection of Melanoides tuberculata (Mollusca: Thiaridae) with Centrocestus formosanus (Trematoda: Heterophyidae) Hudson A. Pinto, Nicole Q. Gonçalves, Danimar López-Hernandez, Eduardo A. Pulido-Murillo, Alan L. Melo, Petr Heneberg PLOS ONE.2018; 13(4): e0194161. CrossRef
Prevalence of Centrocestus formosanus Metacercariae in Ornamental Fish from Chiang Mai, Thailand, with Molecular Approach Using ITS2 Atcharaphan Wanlop, Chalobol Wongsawad, Pongphol Prattapong, Pheravut Wongsawad, Thapana Chontananarth, Jong-Yil Chai The Korean Journal of Parasitology.2017; 55(4): 445. CrossRef
First
Prev
