Acanthamoeba spp. commonly cause Acanthamoeba keratitis which is typically associated with the wear of contact lenses. Therefore, finding an economic, efficient, and safe therapy of natural origin is of outmost importance. This study examined the in vitro lethal potential of ethyl acetate and methanol extracts of Helianthemum lippii (L.) (sun roses) against Acanthamoeba castellanii cysts isolated from patients with amoebic keratitis. Both extracts proved to be potent as regard to their lethal effects on A. castellanii cysts with comparable results to chlorhexidine. The ethyl acetate was more promising with cumulative lethality. It showed a highly significant lethal percentage along the duration of treatment. The analysis of the more potent ethyl acetate extract revealed the presence of 2.96 mg/100 g of total phenolics, 0.289 mg/100 ml of total flavonoids and 37 mg/100 mg of total tannins which highlighted their phytomedicinal role.
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Effectiveness of phytoproducts against pathogenic free-living amoebae - A scoping and critical review paving the way toward plant-based pharmaceuticals Beni Jequicene Mussengue Chaúque, Thaisla Cristiane Borella da Silva, Eduardo Brittes Rott, Felipe Brittes Rott, Ana Paula Marçal Copetti Leite, Guilherme Brittes Benitez, Neuana Fernando Neuana, José Roberto Goldim, Marilise Brittes Rott, Régis Adriel Za Fitoterapia.2025; 182: 106404. CrossRef
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In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp. cysts Ghada Mohamed Kadry, Mousa A. M. Ismail, Nagwa Mostafa El-Sayed, Hanan S. El-Kholy, Dina M. Hamdy El-Akkad Journal of Parasitic Diseases.2021; 45(1): 159. CrossRef
In vitro Propagation to Conserve the Local Endemic and Endangered Medicinal Plant Helianthemum germanicopolitanum Bornm. Emine Kapdan, Mehmet Sezgin Brazilian Archives of Biology and Technology.2021;[Epub] CrossRef
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