Parasites, Hosts and Diseases

"Schistosoma mekongi"

Original Article

Molecular Differentiation of Schistosoma japonicum and Schistosoma mekongi by Real-Time PCR with High Resolution Melting Analysis: Amornmas Kongklieng, Worasak Kaewkong, Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Tongjit Thanchomnang, Viraphong Lulitanond, Pusadee Sri-Aroon, Yanin Limpanont, Wanchai Maleewong; Korean J Parasitol 2013;51(6):651-656.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.651

Abstract
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Human schistosomiasis caused by Schistosoma japonicum and Schistosoma mekongi is a chronic and debilitating helminthic disease still prevalent in several countries of Asia. Due to morphological similarities of cercariae and eggs of these 2 species, microscopic differentiation is difficult. High resolution melting (HRM) real-time PCR is developed as an alternative tool for the detection and differentiation of these 2 species. A primer pair was designed for targeting the 18S ribosomal RNA gene to generate PCR products of 156 base pairs for both species. The melting points of S. japonicum and S. mekongi PCR products were 84.5±0.07℃ and 85.7±0.07℃, respectively. The method permits amplification from a single cercaria or an egg. The HRM real-time PCR is a rapid and simple tool for differentiation of S. japonicum and S. mekongi in the intermediate and final hosts.

Citations

Citations to this article as recorded by

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Mini Review

Modeling the Dynamics and Control of Transmission of Schistosoma japonicum and S. mekongi in Southeast Asia: Hirofumi Ishikawa, Hiroshi Ohmae; Korean J Parasitol 2009;47(1):1-5.
Published online March 12, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.1.1

Abstract
PDF

A mathematical model for transmission of schistosomes is useful to predict effects of various control measures on suppression of these parasites. This review focuses on epidemiological and environmental factors in Schistosoma japonicum and Schistosoma mekongi infections and recent advances in mathematical models of Schistosoma transmission.

Citations

Citations to this article as recorded by

Current advances in serological and molecular diagnosis of Schistosoma mekongi infection
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Tropical Medicine and Health.2024;[Epub] CrossRef
Trematodoses in Cattle and Buffalo Around Schistosomiasis Endemic Areas in Central Sulawesi Province of Indonesia
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O. SANPOOL, P. M. INTAPAN, T. THANCHOMNANG, P. SRI-AROON, V. LULITANOND, L. SADAOW, W. MALEEWONG
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