Parasites, Hosts and Diseases

"Smarn Tesana"

Brief Communications

Update on the risk factors for opisthorchiasis and cholangiocarcinoma in Thailand: Sattrachai Prasopdee, Thittinan Rojthongpond, Yanwadee Chitkoolsamphan, Montinee Pholhelm, Siraphatsorn Yusuk, Junya Pattaraarchachai, Kritiya Butthongkomvong, Jutharat Kulsantiwong, Teva Phanaksri, Anthicha Kunjantarachot, Smarn Tesana, Thanakrit Sathavornmanee, Veerachai Thitapakorn; Parasites Hosts Dis 2023;61(4):463-470.
Published online November 28, 2023; DOI: https://doi.org/10.3347/PHD.23032

Abstract
PDF

This study aimed to identify the recent risk factors for Opisthorchis viverrini infection and cholangiocarcinoma (CCA) to improve disease prevention. The participants were divided into the following 3 groups based on their health status: healthy control (nonOV and nonCCA), those with O. viverrini infection (OV), and those with CCA. A questionnaire was used to explore their lifestyle and behaviors. Multivariate logistic regression and backward elimination were used to identify the significant risk factors. The results showed that the significant risk factors for both O. viverrini infection and CCA were age>50 years (odd ratio (OR)=8.44, p<0.001, 95% confidence intervals (CI) 2.98–23.90 and OR=43.47, p=0.001, 95% CI 14.71–128.45, respectively) and raw fish consumption (OR=8.48, p< 0.001, 95% CI 3.18–22.63 and OR=3.15, p=0.048, 95% CI 1.01–9.86, respectively). A history of O. viverrini infection was identified as an additional risk factor for CCA (OR=20.93, p=0.011, 95% CI 2.04–215.10). This study provided an update on the risk factors for O. viverrini infection and CCA. Asymptomatic patients with O. viverrini infection, particularly those>50 years old, should be carefully monitored to prevent CCA.

Citations

Citations to this article as recorded by

Influence of knowledge, attitude and self‐efficacy on Opisthorchis viverrini and cholangiocarcinoma prevention: A One Health approach in high‐risk areas of Thailand
Worathon Busabong, Nopparat Songserm, Somkiattiyos Woradet, Banchob Sripa
Tropical Medicine & International Health.2025; 30(2): 99. CrossRef
Targeting tumor-associated genes, immune response, and circulating tumor cells in intrahepatic cholangiocarcinoma: Therapeutic potential of Atractylodes lancea (Thunb.) DC
Pongsakorn Martviset, Pathanin Chantree, Nisit Tongsiri, Tullayakorn Plengsuriyakarn, Kesara Na-Bangchang, Keun-Yeong Jeong,
PLOS One.2025; 20(5): e0323732. CrossRef
Large-scale epidemiology of opisthorchiasis in 21 provinces in Thailand based on diagnosis by fecal egg examination and urine antigen assay and analysis of risk factors for infection
Kulthida Y. Kopolrat, Chanika Worasith, Phattharaphon Wongphutorn, Anchalee Techasen, Chatanun Eamudomkarn, Jiraporn Sithithaworn, Watcharin Loilome, Nisana Namwat, Attapol Titapun, Chaiwat Tawarungruang, Bandit Thinkhamrop, Samarn Futrakul, Simon D. Tayl
PLOS Neglected Tropical Diseases.2025; 19(7): e0013095. CrossRef
Chronic opisthorchiasis invasion as a factor aggravating the development of cholangiocellular carcinoma. A literature review
V. V. Ivanov, T. B. Komkova, E. A. Perina, L. Yu. Petrov, E. V. Udut, E. S. Khmelevskaya
Siberian journal of oncology.2025; 24(5): 171. CrossRef
Differential circulating miRNA profiles identified miR-423-5p, miR-93-5p, and miR-4532 as potential biomarkers for cholangiocarcinoma diagnosis
Kittiya Supradit, Sattrachai Prasopdee, Teva Phanaksri, Sithichoke Tangphatsornruang, Montinee Pholhelm, Siraphatsorn Yusuk, Kritiya Butthongkomvong, Kanokpan Wongprasert, Jutharat Kulsantiwong, Amnat Chukan, Smarn Tesana, Veerachai Thitapakorn
PeerJ.2024; 12: e18367. CrossRef

3,611 View
160 Download
4 Web of Science
Crossref

Comparative Characterization of Four Calcium-Binding EF Hand Proteins from Opisthorchis viverrini: Palida Emmanoch, Nanthawat Kosa, Suksiri Vichasri-Grams, Smarn Tesana, Rudi Grams, Amornrat Geadkaew-Krenc; Korean J Parasitol 2018;56(1):81-86.
Published online February 28, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.1.81

Four isoforms of calcium binding proteins containing 2 EF hand motifs and a dynein light chain-like domain in the human liver fluke Opisthorchis viverrini, namely OvCaBP1, 2, 3, and 4, were characterized. They had molecular weights of 22.7, 21.6, 23.7, and 22.5 kDa, respectively and showed 37.2-42.1% sequence identity to CaBP22.8 of O. viverrini. All were detected in 2- and 4-week-old immature and mature parasites. Additionally, OvCaBP4 was found in newly excysted juveniles. Polyclonal antibodies against each isoform were generated to detect the native proteins in parasite extracts by Western blot analysis. All OvCaBPs were detected in soluble and insoluble crude worm extracts and in the excretory-secretory product, at approximate sizes of 21-23 kDa. The ion-binding properties of the proteins were analyzed by mobility shift assays with the divalent cations Ca²⁺, Mg²⁺, Zn²⁺, and Cu²⁺. All OvCaBPs showed mobility shifts with Ca²⁺ and Zn²⁺. OvCaBP1 showed also positive results with Mg²⁺ and Cu²⁺. As tegumental proteins, OvCaBP1, 2, and 3 are interesting drug targets for the treatment of opisthorchiasis.

Citations

Citations to this article as recorded by

Evolutionary analysis of species-specific duplications in flatworm genomes
Mauricio Langleib, Javier Calvelo, Alicia Costábile, Estela Castillo, José F. Tort, Federico G. Hoffmann, Anna V. Protasio, Uriel Koziol, Andrés Iriarte
Molecular Phylogenetics and Evolution.2024; 199: 108141. CrossRef
Cystatins from the Human Liver Fluke Opisthorchis viverrini: Molecular Characterization and Functional Analysis
Amornrat Geadkaew-Krenc, Rudi Grams, Sinee Siricoon, Nanthawat Kosa, Dawid Krenc, Wansika Phadungsil, Pongsakorn Martviset
Pathogens.2023; 12(7): 949. CrossRef
Fasciola gigantica tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes
Muhammad Ehsan, Rui-Si Hu, Jun-Ling Hou, Hany M. Elsheikha, Xiao-Dong Li, Pan-Hong Liang, Xing-Quan Zhu
Parasites & Vectors.2021;[Epub] CrossRef

8,698 View
140 Download
3 Web of Science
Crossref

Original Articles

Molecular and Biochemical Characterization of Opisthorchis viverrini Calreticulin: Wanlapa Chaibangyang, Amornrat Geadkaew-Krenc, Suksiri Vichasri-Grams, Smarn Tesana, Rudi Grams; Korean J Parasitol 2017;55(6):643-652.
Published online December 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.6.643

Abstract
PDF

Calreticulin (CALR), a multifunctional protein thoroughly researched in mammals, comprises N-, P-, and C-domain and has roles in calcium homeostasis, chaperoning, clearance of apoptotic cells, cell adhesion, and also angiogenesis. In this study, the spatial and temporal expression patterns of the Opisthorchis viverrini CALR gene were analyzed, and calcium-binding and chaperoning properties of recombinant O. viverrini CALR (OvCALR) investigated. OvCALR mRNA was detected from the newly excysted juvenile to the mature parasite by RT-PCR while specific antibodies showed a wide distribution of the protein. OvCALR was localized in tegumental cell bodies, testes, ovary, eggs, Mehlis’ gland, prostate gland, and vitelline cells of the mature parasite. Recombinant OvCALR showed an in vitro suppressive effect on the thermal aggregation of citrate synthase. The recombinant OvCALR C-domain showed a mobility shift in native gel electrophoresis in the presence of calcium. The results imply that OvCALR has comparable function to the mammalian homolog as a calcium-binding molecular chaperone. Inferred from the observed strong immunostaining of the reproductive tissues, OvCALR should be important for reproduction and might be an interesting target to disrupt parasite fecundity. Transacetylase activity of OvCALR as reported for calreticulin of Haemonchus contortus could not be observed.

Citations

Citations to this article as recorded by

Recombinant Toxoplasma gondii Calreticulin protein provides partial protection against acute and chronic toxoplasmosis
Jing Zhang, Zhenke Yang, Hangbin Ma, Xiaowei Tian, Jiani Li, Zihang Zhang, Longxin Yang, Mengchuang Feng, Xuefang Mei, Zhenchao Zhang, Xingrui Li, Shuai Wang
Acta Tropica.2023; 241: 106883. CrossRef
The many faces of parasite calreticulin
Diego Esperante, Ana Flisser, Fela Mendlovic
Frontiers in Immunology.2023;[Epub] CrossRef
Two calcium‐binding chaperones from the fat body of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) involved in diapause
Cansu Doğan, Sabine Hänniger, David G. Heckel, Cathy Coutu, Dwayne D. Hegedus, Linda Crubaugh, Russell L. Groves, Şerife Bayram, Umut Toprak
Archives of Insect Biochemistry and Physiology.2021;[Epub] CrossRef
Trypanosoma cruzi Calreticulin: Immune Evasion, Infectivity, and Tumorigenesis
Galia Ramírez-Toloza, Eduardo Sosoniuk-Roche, Carolina Valck, Lorena Aguilar-Guzmán, Viviana P. Ferreira, Arturo Ferreira
Trends in Parasitology.2020; 36(4): 368. CrossRef
The Interactions of Parasite Calreticulin With Initial Complement Components: Consequences in Immunity and Virulence
Galia Ramírez-Toloza, Lorena Aguilar-Guzmán, Carolina Valck, Viviana P. Ferreira, Arturo Ferreira
Frontiers in Immunology.2020;[Epub] CrossRef
Diallyl disulfide down‐regulates calreticulin and promotes C/EBPα expression in differentiation of human leukaemia cells
Jing Sun, Hongxiang Mu, Jia Yu, Linwei Li, Hongxia Yan, Guoqing Li, Hui Tan, Nanyang Yang, Xiaoyan Yang, Lan Yi
Journal of Cellular and Molecular Medicine.2019; 23(1): 194. CrossRef
Evaluation of Opisthorchis viverrini calreticulin for potential host modulation
Wanlapa Chaibangyang, Amornrat Geadkaew-Krenc, Peter M. Smooker, Smarn Tesana, Rudi Grams
Acta Tropica.2018; 187: 175. CrossRef

9,784 View
149 Download
8 Web of Science
Crossref

Distribution and Abundance of Opisthorchis viverrini Metacercariae in Cyprinid Fish in Northeastern Thailand: Somchai Pinlaor, Sudarat Onsurathum, Thidarut Boonmars, Porntip Pinlaor, Nuttanan Hongsrichan, Apisit Chaidee, Ornuma Haonon, Wutipong Limviroj, Smarn Tesana, Sasithorn Kaewkes, Paiboon Sithithaworn; Korean J Parasitol 2013;51(6):703-710.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.703

Abstract
PDF

To increase public health awareness for prevention of opisthorchiasis caused by eating raw freshwater fish, the distribution and abundance of Opisthorchis viverrini metacercariae (OV MC) was investigated in freshwater fish obtained from 20 provinces in northeastern Thailand between April 2011 and February 2012. A cross-sectional survey was conducted on 12,890 fish consisting of 13 species randomly caught from 26 rivers, 10 dams, and 38 ponds/lakes. Fish, were collected in each of the rainy and winter seasons from each province. Fish were identified, counted, weighed, and digested using pepsin-HCl. Samples were examined for OV MC by a sedimentation method, and metacercariae were identified under a stereomicroscope. OV MC were found in 6 species of fish; i.e., Cyclocheilichthys armatus, Puntius orphoides, Hampala dispar, Henicorhynchus siamensis, Osteochilus hasselti, and Puntioplites proctozysron from localities in 13 provinces. Among the sites where OV MC-infected fish were found, 70.0% were dams, 23.7% were ponds/lakes, and 7.7% were rivers. The mean intensity of OV MC ranged from 0.01 to 6.5 cysts per fish (or 1.3-287.5 cysts per kg of fish). A high mean intensity of OV MC per fish (>3 cysts) was found in 5 provinces: Amnat Charoen (6.5 cysts), Nakhon Phanom (4.3), Mukdahan (4.1), Khon Kaen, (3.5) and Si Sa Ket (3.4). In conclusion, OV MC are prevalent in natural cyprinid fish, with the infection rate varying according to fish species and habitats.

Citations

Citations to this article as recorded by

Spatial prediction of the probability of liver fluke infection using a geographic weighted regression (GWR) model in waterways connecting the Mekong River, Sakon Nakhon of Thailand
Benjamabhorn Pumhirunroj, Patiwat Littidej, Thidarut Boonmars, Atchara Artchayasawat, Nutchanat Buasri, Donald Slack
One Health.2026; : 101320. CrossRef
High-fat/high-fructose diet and Opisthorchis viverrini infection promote metabolic dysfunction-associated steatotic liver disease via inflammation, fibrogenesis, and metabolic dysfunction
Lakhanawan Charoensuk, Phonpilas Thongpon, Chutima Sitthirach, Apisit Chaidee, Kitti Intuyod, Chawalit Pairojkul, Ei Htet Htet Khin, Chanakan Jantawong, Kanjana Thumanu, Porntip Pinlaor, Nuttanan Hongsrichan, Somchai Pinlaor
Acta Tropica.2025; 261: 107491. CrossRef
Development of a Dielectric Heating System for Selective Thermal Targeting of Liver Fluke Regions in Cirrhinus microlepis
Supatinee Kornsing, Sirigiet Phunklang, Chanchai Thongsopa, Piyaporn Krachodnok, Nuchanart Santalunai, Samran Santalunai
Applied Sciences.2025; 15(10): 5466. CrossRef
Distribution of Opisthorchis viverrini metacercariae and cyprinoid fish abundance in Nong Han Wetland, Thailand
Wiwat Kaensa, Lakhanawan Charoensuk, Chadon Nakmai, Somchai Pinlaor, Apiporn T. Suwannatrai, Umawadee Laothong, Suksanti Prakobwong
Parasite Epidemiology and Control.2025; 30: e00447. CrossRef
Unpacking the black box: I. Knowledge production and public health narratives on liver fluke infection and cholangiocarcinoma in Northeast Thailand
Yee Ting Lee, Alan D. Ziegler
South East Asia Research.2025; 33(2): 133. CrossRef
Gene Editing of a Carcinogenic Liver Fluke Tetraspanin Impairs Parasite Surface Biogenesis and Extracellular Vesicle Uptake by Human Host Cells
Sujittra Chaiyadet, Wannaporn Ittiprasert, Michael J Smout, Ladawan Khowawisetsut, Apisit Ruangsuwast, Paul J Brindley, Alex Loukas, Thewarach Laha
The Journal of Infectious Diseases.2025;[Epub] CrossRef
Transcriptomics analysis of in vivo Opisthorchis viverrini: Stage-specific gene expression and novel genes with stable expression in mammalian stages for opisthorchiasis intervention
Waraporn Taweesin, Siriyakorn Kulwong, Wassana Jamnongkan, Wichit Taron, Autchasai Siriprayong, Sirinya Sitthirak, Anchalee Techasen, Opal Pitaksakulrat, Nisana Namwat, Poramate Klanrit, Watcharin Loilome, Arporn Wangwiwatsin, Eduardo José Lopes-Torres
PLOS Neglected Tropical Diseases.2025; 19(12): e0013714. CrossRef
Linking Parasite, People and Places: The Food Networks of Liver Fluke Infection
Yi-Chen Wang, Andrea Law, Jutamas Namsanor, Paiboon Sithithaworn, Narong Khuntikeo
Annals of the American Association of Geographers.2024; 114(8): 1781. CrossRef
Opisthorchis viverrini excretory-secretory products suppress GLUT8 of cholangiocytes
Sandy Kha, Sujittra Chaiyadet, Prasert Saichua, Sirikachorn Tangkawatana, Banchob Sripa, Sutas Suttiprapa
Parasitology Research.2024;[Epub] CrossRef
Hight prevalent of Opisthorchis viverrini infection and coincident Haplorchis metacercariae in cyprinid fishes in upper northeastern region of Thailand
Naiyana Senasri, Nattiya Chumnanka, Patcharawalai Sriyasak, Supannee Suwanpakdee, Sugunya Kumla, Kosit Sreeputhorn
Parasite Epidemiology and Control.2024; 25: e00348. CrossRef
Exploring the second intermediate hosts and morphology of human- and cat-specific Opisthorchis viverrini-like populations
Vania Agustina, Prasert Saichua, Thewarach Laha, Sirikachorn Tangkawatana, Suksanti Prakobwong, Nonglak Laoprom, Wanrak Kamphasri, Chonteera Chareonchai, David Blair, Sutas Suttiprapa
International Journal for Parasitology.2024; 54(10): 497. CrossRef
Integrated One-Health approach for prevention and control of Opisthorchis viverrini infection in rural Thailand: a 3-year study
Lakhanawan Charoensuk, Kacha Chedtabud, Suwit Chaipibool, Umawadee Laothong, Apiporn Suwannatrai, Somchai Pinlaor, Suksanti Prakobwong
Parasitology Research.2024;[Epub] CrossRef
Spatial Predictive Modeling of Liver Fluke Opisthorchis viverrine (OV) Infection under the Mathematical Models in Hexagonal Symmetrical Shapes Using Machine Learning-Based Forest Classification Regression
Benjamabhorn Pumhirunroj, Patiwat Littidej, Thidarut Boonmars, Atchara Artchayasawat, Narueset Prasertsri, Phusit Khamphilung, Satith Sangpradid, Nutchanat Buasri, Theeraya Uttha, Donald Slack
Symmetry.2024; 16(8): 1067. CrossRef
Transcriptome changes of liver fluke Opisthorchis viverrini in diabetic hamsters
Apisit Chaidee, Naruechar Charoenram, Chatchawan Sengthong, Rungtiwa Dangtakot, Porntip Pinlaor, Thatsanapong Pongking, Somchai Pinlaor
Parasite.2024; 31: 54. CrossRef
Spatial prediction of the probability of liver fluke infection in water resource within sub-basin using an optimized geographically-weighted regression model
Benjamabhorn Pumhirunroj, Patiwat Littidej, Thidarut Boonmars, Atchara Artchayasawat, Nutchanat Buasri, Donald Slack
Frontiers in Veterinary Science.2024;[Epub] CrossRef
Temperature and Preparation Time Intervals on Survival of Opisthorchis viverrini Metacercariae in Pickled Fish (Pla‐som)
Naiyana Senasri, Nattiya Chumnanka, Wiboonsuk Talkul, Zheng-Fei Yan
International Journal of Food Science.2024;[Epub] CrossRef
Re‐evaluation of certain aspects of the EFSA Scientific Opinion of April 2010 on risk assessment of parasites in fishery products, based on new scientific data. Part 2
Ana Allende, Avelino Alvarez‐Ordóñez, Valeria Bortolaia, Sara Bover‐Cid, Alessandra De Cesare, Wietske Dohmen, Laurent Guillier, Lieve Herman, Liesbeth Jacxsens, Maarten Nauta, Lapo Mughini‐Gras, Jakob Ottoson, Luisa Peixe, Fernando Perez‐Rodriguez, Panag
EFSA Journal.2024;[Epub] CrossRef
A study protocol for a multi-country cluster randomized controlled trial of the impact of a multi-component One Health strategy to eliminate Opisthorchis viverrini and soil transmitted helminths in the Lower Mekong Basin
Mary Lorraine Mationg, Archie C. A. Clements, Gail M. Williams, Matthew Kelly, Donald E. Stewart, Catherine A. Gordon, Kinley Wangdi, Sirikachorn Tangkawattana, Apiporn T. Suwannatrai, Vanathom Savathdy, Visal Khieu, Sangduan Wannachart, Suji Yoo O’Connor
Trials.2024;[Epub] CrossRef
Examining ecosystem (dis-)services on liver fluke infection in rural Northeast Thailand
Yi-Chen Wang, Andrea Law, Jutamas Namsanor, Paiboon Sithithaworn
Infectious Diseases of Poverty.2023;[Epub] CrossRef
Modelling morbidity for neglected tropical diseases: the long and winding road from cumulative exposure to long-term pathology
Anna Borlase, Joaquin M. Prada, Thomas Crellen
Philosophical Transactions of the Royal Society B.2023;[Epub] CrossRef
Machine-Learning-Based Forest Classification and Regression (FCR) for Spatial Prediction of Liver Fluke Opisthorchis viverrini (OV) Infection in Small Sub-Watersheds
Benjamabhorn Pumhirunroj, Patiwat Littidej, Thidarut Boonmars, Kanokwan Bootyothee, Atchara Artchayasawat, Phusit Khamphilung, Donald Slack
ISPRS International Journal of Geo-Information.2023; 12(12): 503. CrossRef
Infection rate of Opisthorchis viverrini metacercariae in cyprinoid fish from the markets and its association to human opisthorchiasis in the local community in the Northeast Thailand
Lakhanawan Charoensuk, Alexis Ribas, Kacha Chedtabud, Suksanti Prakobwong
Acta Tropica.2022; 225: 106216. CrossRef
High diversity of trematode metacercariae that parasitize freshwater gastropods in Bangkok, Thailand, and their infective situations, morphologies and phylogenetic relationships
Pichit Wiroonpan, Thapana Chontananarth, Jong-Yil Chai, Watchariya Purivirojkul
Parasitology.2022; 149(7): 913. CrossRef
Knockout of liver fluke granulin, Ov-grn-1, impedes malignant transformation during chronic infection with Opisthorchis viverrini
Sujittra Chaiyadet, Sirikachorn Tangkawattana, Michael J. Smout, Wannaporn Ittiprasert, Victoria H. Mann, Raksawan Deenonpoe, Patpicha Arunsan, Alex Loukas, Paul J. Brindley, Thewarach Laha, Kenneth D. Vernick
PLOS Pathogens.2022; 18(9): e1010839. CrossRef
Curcumin-loaded nanocomplexes ameliorate the severity of nonalcoholic steatohepatitis in hamsters infected with Opisthorchis viverrini
Chutima Sitthirach, Lakhanawan Charoensuk, Chawalit Pairojkul, Apisit Chaidee, Kitti Intuyod, Thatsanapong Pongking, Phonpilas Thongpon, Chanakan Jantawong, Nuttanan Hongsrichan, Sakda Waraasawapati, Manachai Yingklang, Somchai Pinlaor, Michael W. Greene
PLOS ONE.2022; 17(9): e0275273. CrossRef
Association of Red Blood Cell Life Span with Abnormal Changes in Cardiac Structure and Function in Non-Dialysis Patients with Chronic Kidney Disease Stages 3–5
Siyi Rao, Jing Zhang, Jiaqun Lin, Jianxin Wan, Yi Chen
Journal of Clinical Medicine.2022; 11(24): 7373. CrossRef
Very low prevalence of Opisthorchis viverrini s.l. cercariae in Bithynia siamensis siamensis snails from the canal network system in the Bangkok Metropolitan Region, Thailand
Phuphitchan Rachprakhon, Watchariya Purivirojkul
Parasite.2021; 28: 2. CrossRef
Current status of human liver fluke infections in the Greater Mekong Subregion
Banchob Sripa, Apiporn T. Suwannatrai, Somphou Sayasone, Dung Trung Do, Virak Khieu, Yichao Yang
Acta Tropica.2021; 224: 106133. CrossRef
Infection Dynamics of Opisthorchis viverrini Metacercariae in Cyprinid Fishes from Two Endemic Areas in Thailand and Lao PDR
Jutamas Namsanor, Nadda Kiatsopit, Thewarach Laha, Ross H. Andrews, Trevor N. Petney, Paiboon Sithithaworn
The American Journal of Tropical Medicine and Hygiene.2020; 102(1): 110. CrossRef
Digenean Holostephanus (Trematoda: Digenea: Cyathocotylidae) metacercariae in common carp (Cyprinus carpio Linnaeus, 1758) muscle: zoonotic potential and sensitivity to physico-chemical treatments
D. Sándor, M. Gyöngy, K. Nyeste, I. Czeglédi, C. Székely, K. Buchmann, G. Cech
Journal of Helminthology.2020;[Epub] CrossRef
Discovery of Carcinogenic Liver Fluke Metacercariae in Second Intermediate Hosts and Surveillance on Fish-Borne Trematode Metacercariae Infections in Mekong Region of Myanmar
Ei Ei Phyo Myint, Amornpun Sereemaspun, Joacim Rocklöv, Choosak Nithikathkul
International Journal of Environmental Research and Public Health.2020; 17(11): 4108. CrossRef
Reduction of Reinfection Rates with Opisthorchis viverrini through a Three-Year Management Program in Endemic Areas of Northeastern Thailand
Suksanti Prakobwong, Kulwadee Suwannatrai
The Korean Journal of Parasitology.2020; 58(5): 527. CrossRef
Programmed knockout mutation of liver fluke granulin attenuates virulence of infection-induced hepatobiliary morbidity
Patpicha Arunsan, Wannaporn Ittiprasert, Michael J Smout, Christina J Cochran, Victoria H Mann, Sujittra Chaiyadet, Shannon E Karinshak, Banchob Sripa, Neil David Young, Javier Sotillo, Alex Loukas, Paul J Brindley, Thewarach Laha
eLife.2019;[Epub] CrossRef
A comprehensive review of omics and host-parasite interplays studies, towards control of Opisthorchis viverrini infection for prevention of cholangiocarcinoma
Sattrachai Prasopdee, Veerachai Thitapakorn, Thanakrit Sathavornmanee, Smarn Tesana
Acta Tropica.2019; 196: 76. CrossRef
Modeling liver fluke transmission in northeast Thailand: Impacts of development, hydrology, and control
Tomás M. León, Travis C. Porco, Christina S. Kim, Sasithorn Kaewkes, Wanlop Kaewkes, Banchob Sripa, Robert C. Spear
Acta Tropica.2018; 188: 101. CrossRef
The rapid detection method by polymerase chain reaction for minute intestinal trematodes: Haplorchis taichui in intermediate snail hosts based on 18s ribosomal DNA
Thapana Chontananarth, Sothorn Anucherngchai, Thanawan Tejangkura
Journal of Parasitic Diseases.2018; 42(3): 423. CrossRef
New locality record for Haplorchoides mehrai and possible interactions with Opisthorchis viverrini metacercariae in cyprinid fishes in Northeast Thailand
Yupin Manpratum, Wanlop Kaewkes, Pierre Echaubard, Banchob Sripa, Sasithorn Kaewkes
Parasitology Research.2017; 116(2): 601. CrossRef
Effects of albendazole, artesunate, praziquantel and miltefosine, on Opisthorchis viverrini cercariae and mature metacercariae
Phornphitcha Pechdee, Monticha Chaiyasaeng, Chanisala Sereewong, Jukkrid Chaiyos, Apiporn Suwannatrai, Sutee Wongmaneeprateep, Smarn Tesana
Asian Pacific Journal of Tropical Medicine.2017; 10(2): 126. CrossRef
Ultrastructural characters of the spermatozoon of the liver fluke Opisthorchis viverrini (Poirier, 1886) (Opisthorchiidae)
Jordi Miquel, Zdzisław Świderski, Banchob Sripa, Alexis Ribas
Parasitology Research.2017; 116(9): 2499. CrossRef
Epidemiology of Opisthorchis viverrini in an endemic area of Thailand, an integrative approach
S. Prakobwong, W. Gunnula, S. Chaipibool, B. Nimala, J. Sangthopo, N. Sirivetthumrong, A. Ribas
Helminthologia.2017; 54(4): 298. CrossRef
Contamination of Opisthorchis viverrini and Haplorchis taichui metacercariae in fermented fish products in northeastern Thailand markets
Sudarat Onsurathum, Porntip Pinlaor, Lakhanawan Charoensuk, Ornuma Haonon, Apisit Chaidee, Kitti Intuyod, Porntip Laummaunwai, Thidarut Boonmars, Wanlop Kaewkes, Somchai Pinlaor
Food Control.2016; 59: 493. CrossRef
Effects of fermentation time and low temperature during the production process of Thai pickled fish (pla-som) on the viability and infectivity of Opisthorchis viverrini metacercariae
Sudarat Onsurathum, Porntip Pinlaor, Ornuma Haonon, Apisit Chaidee, Lakhanawan Charoensuk, Kitti Intuyod, Thidarut Boonmars, Porntip Laummaunwai, Somchai Pinlaor
International Journal of Food Microbiology.2016; 218: 1. CrossRef
Comparison of infectivity, metacercarial burden and host mortality induced byOpisthorchis viverrinisensu lato cercariae from Lao PDR compared with Thailand in cyprinid fish,Barbonymus gonionotus
Kulthida Kopolrat, Paiboon Sithithaworn, Nadda Kiatsopit, Opal Pitaksakulrat, Smarn Tesana, Ross H. Andrews, Trevor N. Petney
Transactions of The Royal Society of Tropical Medicine and Hygiene.2016; 110(1): 46. CrossRef
Plasma Peptidome as a Source of Biomarkers for Diagnosis of Cholangiocarcinoma
Kanawut Kotawong, Veerachai Thitapakorn, Sittiruk Roytrakul, Narumon Phaonakrop, Vithoon Viyanant, Kesara Na-Bangchang
Asian Pacific Journal of Cancer Prevention.2016; 17(3): 1163. CrossRef
Chronic Opisthorchis viverrini Infection Changes the Liver Microbiome and Promotes Helicobacter Growth
Upsornsawan Itthitaetrakool, Porntip Pinlaor, Somchai Pinlaor, Chariya Chomvarin, Rungtiwa Dangtakot, Apisit Chaidee, Chotechana Wilailuckana, Arunnee Sangka, Aroonlug Lulitanond, Puangrat Yongvanit, Paulo Lee Ho
PLOS ONE.2016; 11(11): e0165798. CrossRef
Opisthorchiasis in Northeastern Thailand: Effect of local environment and culture
Beuy Joob, Viroj Wiwanitkit
Asian Pacific Journal of Tropical Disease.2015; 5: S96. CrossRef

13,715 View
186 Download
Crossref

Histopathological Changes in Tissues of Bithynia siamensis goniomphalos Incubated in Crude Extracts of Camellia Seed and Mangosteen Pericarp: Ratchadawan Aukkanimart, Thidarut Boonmars, Somchai Pinlaor, Smarn Tesana, Surasit Aunpromma, Chantana Booyarat, Pranee Sriraj, Porntip Laummaunwai, Wiyada Punjaruk; Korean J Parasitol 2013;51(5):537-544.
Published online October 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.5.537

Abstract
PDF

The present study was performed to observe histopathological changes in tissues of Bithynia siamensis goniomphalos (Gastropoda, Bithyniidae) incubated in crude extract solutions of camellia (Camellia oleifera) seed and mangosteen (Garcinia mangostana) pericarp, and furthermore to estimate the molluscicidal effects of 2 plant substances. Substantial numbers of bithyniid snails were incubated in various concentrations of 2 plant solution for 24 hr. As the positive control, snails incubated in various concentrations of niclosamide, a chemical molluscicide, were used. The histopathological findings were observed in sectioned snail specimens of each experimental and control groups. The results showed that both camellia and mangosteen extracts had molluscicidal effects at 24 hr with 50% lethal concentration (LC₅₀) at concentrations of 0.003 and 0.002 g/ml, respectively, while niclosamide had LC₅₀ at concentrations 0.599 ppm. B. siamensis goniomphalos snail tissues (foot, gill, and digestive system) showed disruption of columnar muscle fibers of the foot, reduction of the length and number of gill cilia, numerous mucous vacuoles, and irregularly shaped of epithelial cells. Irregular apical and calciferous cells, dilatation of the digestive gland tubule, and large hemolymphatic spaces, and irregular apical surfaces, detachment of cilia, and enlargement of lysosomal vacuoles of epidermis were also shown in all groups. By the present study, it is confirmed that 2 plants, camellia and mangosteen, are keeping some substance having molluscicidal effects, and histopathological findings obtained in this study will provide some clues in further studies on their action mechanisms to use them as natural molluscicides.

Citations

Citations to this article as recorded by

Molluscicidal property of symbiotic bacteria associated with entomopathogenic nematodes against Indoplanorbis exustus and Radix rubiginosa, the intermediate hosts of trematode parasites
Abdulhakam Dumidae, Chanatinart Homkeaw, Chanakan Subkrasae, Jiranun Ardpairin, Supawan Pansri, Raxsina Polseela, Ittipon Phoungpetchara, Tewarat Kumchantuek, Sarunporn Tandhavanan, Aunchalee Thanwisai, Apichat Vitta
Parasite Epidemiology and Control.2024; 27: e00375. CrossRef
Symbiotic bacteria associated with entomopathogenic nematodes showed molluscicidal activity against Biomphalaria glabrata, an intermediate host of Schistosoma mansoni
Jiranun Ardpairin, Chanakan Subkrasae, Abdulhakam Dumidae, Supawan Pansri, Chanatinat Homkaew, Wipanee Meesil, Tewarat Kumchantuek, Ittipon Phoungpetchara, Adler R. Dillman, Coralie Pavesi, Helge B. Bode, Sarunporn Tandhavanant, Aunchalee Thanwisai, Apich
Parasites & Vectors.2024;[Epub] CrossRef
Biocontrol of snail-borne parasites with the glossiphoniid leech, Helobdella austinensis
Naim Saglam, Diana S. Melissaratos, Daniel H. Shain
Biology Letters.2023;[Epub] CrossRef
Research progress and management strategies of fungal diseases in Camellia oleifera
Xingzhou Chen, Yuan He, Zhikai Wang, Anqi Niu, Yi Xue, Diao Zhou, Guoying Zhou, Junang Liu
Frontiers in Microbiology.2023;[Epub] CrossRef
Snail-borne parasitic diseases: an update on global epidemiological distribution, transmission interruption and control methods
Xiao-Ting Lu, Qiu-Yun Gu, Yanin Limpanont, Lan-Gui Song, Zhong-Dao Wu, Kamolnetr Okanurak, Zhi-Yue Lv
Infectious Diseases of Poverty.2018;[Epub] CrossRef
Toxic effect of clove oil on the survival and histology of various tissues of pestiferous land snail Achatina fulica (Bowdich, 1822)
Yadnya A. Parvate, Liji Thayil
Journal of Experimental Biology and Agricultural Sciences.2017; 5(4): 492. CrossRef
Linalool, derived from Cinnamomum camphora (L.) Presl leaf extracts, possesses molluscicidal activity against Oncomelania hupensis and inhibits infection of Schistosoma japonicum
Fan Yang, Erping Long, Juhua Wen, Lei Cao, Chengcheng Zhu, Huanxin Hu, Ying Ruan, Kamolnetr Okanurak, Huiling Hu, Xiaoxia Wei, Xiangyun Yang, Chaofan Wang, Limei Zhang, Xiaoying Wang, Pengyu Ji, Huanqin Zheng, Zhongdao Wu, Zhiyue Lv
Parasites & Vectors.2014;[Epub] CrossRef

10,952 View
139 Download
Crossref

Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda): Min-Ho Choi, Jin-Sook Ryu, Mejeong Lee, Shunyu Li, Byung-Suk Chung, Jong-Yil Chai, Paiboon Sithithaworn, Smarn Tesana, Sung-Tae Hong; Korean J Parasitol 2003;41(3):155-163.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.155

Abstract
PDF

The antigenic characterizations and serological reactions of human liver flukes, Clonorchis sinensis and Opisthorchis viverrini, were analyzed by immunoblot. The antigenic profiles of the crude extract of Clonorchis contained major proteins of 8, 26-28, 34-37, 43, and 70 kDa, and those of Opisthorchis 34-37, 43, 70, and 100 kDa. Of these, the 8, 26-28 and 34-37 kDa bands of Clonorchis and the 100 kDa of Opisthorchis were major components of each excretory-secretory antigen. The 8 and 26-28 kDa bands were specific to Clonorchis but the 100 kDa of Opisthorchis cross-reacted with the sera of clonorchiasis, and the 34-37, 70 and 100 kDa bands cross-reacted with sera of other helminthiases. The frequency and intensity of the immunoblot reactions were positively correlated with the intensity of the liver fluke infection.

Citations

Citations to this article as recorded by

Construction and validation of a novel multi-epitope in silico vaccine design against the paramyosin protein of Opisthorchis viverrini using immunoinformatics analyses
Alok Kafle, Jan Clyden B. Tenorio, Roshan Kumar Mahato, Sahara Dhakal, Muhammad F. Heikal, Sutas Suttiprapa
Acta Tropica.2024; 260: 107389. CrossRef
Antigenic components, identification, and characterization of whole worm extract of Platynosomum illiciens
Shin Moe Aoke, Amaya Watthanadirek, Napassorn Poolsawat, Nitipon Srionrod, Pornpiroon Nooroong, Sutthida Minsakorn, Sitthichok Lacharoje, Woraporn Sukhumavasi, Panat Anuracpreeda
Veterinary Parasitology.2022; 310: 109774. CrossRef
A first attempt at determining the antibody-specific pattern of Platynosomum fastosum crude antigen and identification of immunoreactive proteins for immunodiagnosis of feline platynosomiasis
Babi Kyi Soe, Poom Adisakwattana, Onrapak Reamtong, Panat Anuracpreeda, Woraporn Sukhumavasi
Veterinary World.2022; : 2029. CrossRef
Current Perspectives on Opisthorchiasis Control and Cholangiocarcinoma Detection in Southeast Asia
Narong Khuntikeo, Attapol Titapun, Watcharin Loilome, Puangrat Yongvanit, Bandit Thinkhamrop, Nittaya Chamadol, Thidarat Boonmars, Teerachai Nethanomsak, Ross H. Andrews, Trevor N. Petney, Paiboon Sithithaworn
Frontiers in Medicine.2018;[Epub] CrossRef
Screening US Vietnam Veterans for Liver Fluke Exposure 5 Decades After the End of the War
George Psevdos, Florence M. Ford, Sung-Tae Hong
Infectious Diseases in Clinical Practice.2018; 26(4): 208. CrossRef
Comments on “Screening US Vietnam Veterans for Liver Fluke Exposure 5 Decades After the End of the War”
Theodore E. Nash, David Sullivan, Edward Mitre, Eric Garges, Victoria J. Davey, Gary Roselle, Stephen J. Davies, Peter D. Rumm
Infectious Diseases in Clinical Practice.2018; 26(4): 240. CrossRef
Responding to Letter “Comments on ‘Screening US Vietnam Veterans for Liver Fluke Exposure 5 Decades After the End of the War’” by Theodore E. Nash, David Sullivan, Edward Mitre, et al
George Psevdos, Florence M. Ford, Sung-Tae Hong
Infectious Diseases in Clinical Practice.2018; 26(4): 242. CrossRef
Characterization of immunogenic Clonorchis sinensis protein fractions by gel filtration chromatography
Duan Pham Ngoc, Yuji Arimatsu, Sasithorn Kaewkes, Banchob Sripa
Asian Pacific Journal of Tropical Disease.2015; 5(4): 284. CrossRef
Eosinophilia in Infectious Diseases
Elise M. O’Connell, Thomas B. Nutman
Immunology and Allergy Clinics of North America.2015; 35(3): 493. CrossRef
Predominance of IL-10 and TGF-β production from the mouse macrophage cell line, RAW264.7, in response to crude antigens from Clonorchis sinensis
Hae Joo Wi, Yan Jin, Min-Ho Choi, Sung-Tae Hong, Young Mee Bae
Cytokine.2012; 59(2): 237. CrossRef
The identification of antigenic proteins: 14-3-3 protein and propionyl-CoA carboxylase in Clonorchis sinensis
Myoung-Ro Lee, Yu-Jung Kim, Dae-Won Kim, Won Gi Yoo, Shin-Hyeong Cho, Kwang Yeon Hwang, Jung-Won Ju, Won-Ja Lee
Molecular and Biochemical Parasitology.2012; 182(1-2): 1. CrossRef
Detection of salivary antibodies to crude antigens of Opisthorchis viverrini in opisthorchiasis and cholangiocarcinoma patients
Ponlatham Chaiyarit, Paiboon Sithithaworn, Chanitra Thuwajit, Puangrat Yongvanit
Clinical Oral Investigations.2011; 15(4): 477. CrossRef
Clonorchis sinensis infection is positively associated with atopy in endemic area
M‐H Choi, Y‐S Chang, M. K. Lim, Y. M. Bae, S‐T Hong, J‐K Oh, E. H. Yun, M‐J Bae, H‐S Kwon, S‐M Lee, H‐W Park, K‐U Min, Y‐Y Kim, S‐H Cho
Clinical & Experimental Allergy.2011; 41(5): 697. CrossRef
Identification of a serodiagnostic antigen, legumain, by immunoproteomic analysis of excretory‐secretory products of Clonorchis sinensis adult worms
Jung‐Won Ju, Hyun‐Na Joo, Myoung‐Ro Lee, Shin‐Hyeong Cho, Hyeng‐Il Cheun, Jung‐Yeon Kim, Young‐Hee Lee, Kwang‐Jun Lee, Woon‐Mok Sohn, Dong‐Min Kim, Il‐Chul Kim, Byoung Chul Park, Tong‐Soo Kim
PROTEOMICS.2009; 9(11): 3066. CrossRef
Resistance of cholangiocarcinoma cells to parthenolide-induced apoptosis by the excretory–secretory products of Clonorchis sinensis
Young Ju Kim, Min-Ho Choi, Sung-Tae Hong, Young Mee Bae
Parasitology Research.2009; 104(5): 1011. CrossRef
Serodiagnostic applicability of recombinant antigens of Clonorchis sinensis expressed by wheat germ cell-free protein synthesis system
Chenghua Shen, Jong-Ae Lee, Sonia Refaat Ahmed Allam, Young Mee Bae, Eun-Taek Han, Satoru Takeo, Takafumi Tsuboi, Sung-Tae Hong, Min-Ho Choi
Diagnostic Microbiology and Infectious Disease.2009; 64(3): 334. CrossRef
Proliferative effects of excretory/secretory products from Clonorchis sinensis on the human epithelial cell line HEK293 via regulation of the transcription factor E2F1
Young Ju Kim, Min-Ho Choi, Sung-Tae Hong, Young Mee Bae
Parasitology Research.2008; 102(3): 411. CrossRef
A Bile Based Study of Clonorchis sinensis Infections in Patients with Biliary Tract Diseases in Ulsan, Korea
Kwang Ro Joo, Sung-Jo Bang
Yonsei Medical Journal.2005; 46(6): 794. CrossRef
Organ-specific antigens of Clonorchis sinensis
Shunyu Li, Byung-Suk Chung, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2004; 42(4): 169. CrossRef
Evaluation of Clonorchis sinensis Recombinant 7-Kilodalton Antigen for Serodiagnosis of Clonorchiasis
Qin-Ping Zhao, Sung-Ung Moon, Hyeong-Woo Lee, Byoung-Kuk Na, Seung-Yull Cho, Yoon Kong, Ming-Sen Jiang, Ai-Hua Li, Tong-Soo Kim
Clinical and Vaccine Immunology.2004; 11(4): 814. CrossRef