Parasites, Hosts and Diseases

"Tae Im Kim"

Original Articles

Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets: Won Gi Yoo, Sun-Min Kim, Eun Jeong Won, Ji-Yun Lee, Fuhong Dai, Ho Choon Woo, Ho-Woo Nam, Tae Im Kim, Jeong-Hee Han, Dongmi Kwak, Yun Sang Cho, Seung-Won Kang, Tong-Soo Kim, Xing-Quan Zhu, Chunren Wang, Heejeong Youn, Sung-Jong Hong; Korean J Parasitol 2018;56(5):437-446.
Published online October 31, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.5.437

Abstract
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To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×103 or 1.0×104 tachyzoites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066-0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative predictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a commercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to supply safe pork on the tables.

Citations

Citations to this article as recorded by

Molecular survey of Toxoplasma gondii B1 gene in pigs from various localities in Korea
Dongmi Kwak, Min-Goo Seo
Parasites, Hosts and Diseases.2024; 62(3): 294. CrossRef
Foodborne transmission of Toxoplasma gondii infection in the last decade. An overview
S. Almeria, J.P. Dubey
Research in Veterinary Science.2021; 135: 371. CrossRef
Descriptive Comparison of ELISAs for the Detection of Toxoplasma gondii Antibodies in Animals: A Systematic Review
K. L. D. Tharaka D. Liyanage, Anke Wiethoelter, Jasmin Hufschmid, Abdul Jabbar
Pathogens.2021; 10(5): 605. CrossRef
All about Toxoplasma gondii infections in pigs: 2009–2020
Jitender P. Dubey, Camila K. Cerqueira-Cézar, Fernando H.A. Murata, Oliver C.H. Kwok, Dolores Hill, Yurong Yang, Chunlei Su
Veterinary Parasitology.2020; 288: 109185. CrossRef
WITHDRAWN: All about Toxoplasma gondii infections in pigs: the past decade
Jitender P. Dubey, Camila K. Cerqueira-Cézar, Fernando H.A. Murata, Oliver C.H. Kwok, Dolores Hill, Yurong R. Yang, Chunlei Su
Veterinary Parasitology.2020; : 109182. CrossRef

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PCR-RFLP for Rapid Subtyping of Plasmodium vivax Korean Isolates: Jung-Mi Kang, Jinyoung Lee, Tae Im Kim, Eun-Ha Koh, Tong-Soo Kim, Woon-Mok Sohn, Byoung-Kuk Na; Korean J Parasitol 2017;55(2):159-165.
Published online April 30, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.2.159

Abstract
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Vivax malaria reemerged in Korea in 1993 and the outbreak has been continued with fluctuating numbers of annual indigenous cases. Understanding the nature of the genetic population of Plasmodium vivax circulating in Korea is beneficial for the knowledge of the nationwide parasite heterogeneity and in the implementation of malaria control programs in the country. Previously, we analyzed polymorphic nature of merozoite surface protein-1 (MSP-1) and MSP-3α in Korean P. vivax population and identified the Korean P. vivax population has been diversifying rapidly, with the appearance of parasites with new genetic subtypes, despite the recent reduction of the disease incidence. In the present study, we developed simple PCR-RFLP methods for rapid subtyping of MSP-1 and MSP-3α of Korean P. vivax isolates. These PCR-RFLP methods were able to easily distinguish each subtype of Korean P. vivax MSP-1 and MSP-3α with high accuracy. The PCR-RFLP subtyping methods developed here would be easily applied to massive epidemiological studies for molecular surveillance to understand genetic population of P. vivax and to supervise the genetic variation of the parasite circulating in Korea.

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Molecular Profiles of Antimalarial Drug Resistance in Plasmodium Species from Asymptomatic Malaria Carriers in Gia Lai Province, Vietnam
Hương Giang Lê, Tuấn Cường Võ, Jung-Mi Kang, Chau Van Khanh, Nguyen Thi Minh Trinh, Nguyen Thi Lien Hanh, Minkyoung Cho, Huynh Hong Quang, Byoung-Kuk Na
Microorganisms.2025; 13(9): 2101. CrossRef
Molecular surveillance of malaria in the Central Highlands, Vietnam
Tuấn Cường Võ, Hương Giang Lê, Jung-Mi Kang, Haung Naw, Chia-Kwung Fan, Nguyen Thi Minh Trinh, Huynh Hong Quang, Byoung-Kuk Na
Parasitology International.2021; 83: 102374. CrossRef

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Identification and Molecular Characterization of Parkin in Clonorchis sinensis: Xuelian Bai, Tae Im Kim, Ji-Yun Lee, Fuhong Dai, Sung-Jong Hong; Korean J Parasitol 2015;53(1):65-75.
Published online February 27, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.1.65

Abstract
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Clonorchis sinensis habitating in the bile duct of mammals causes clonorchiasis endemic in East Asian countries. Parkin is a RING-between-RING protein and has E3-ubiquitin ligase activity catalyzing ubiquitination and degradation of substrate proteins. A cDNA clone of C. sinensis was predicted to encode a polypeptide homologous to parkin (CsParkin) including 5 domains (Ubl, RING0, RING1, IBR, and RING2). The cysteine and histidine residues binding to Zn2⁺ were all conserved and participated in formation of tertiary structural RINGs. Conserved residues were also an E2-binding site in RING1 domain and a catalytic cysteine residue in the RING2 domain. Native CsParkin was determined to have an estimated molecular weight of 45.7 kDa from C. sinensis adults by immunoblotting. CsParkin revealed E3-ubiquitin ligase activity and higher expression in metacercariae than in adults. CsParkin was localized in the locomotive and male reproductive organs of C. sinensis adults, and extensively in metacercariae. Parkin has been found to participate in regulating mitochondrial function and energy metabolism in mammalian cells. From these results, it is suggested that CsParkin play roles in energy metabolism of the locomotive organs, and possibly in protein metabolism of the reproductive organs of C. sinensis.

Citations

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Characterization of a novel organic solute transporter homologue from Clonorchis sinensis
Yanyan Lu, Won Gi Yoo, Fuhong Dai, Ji-Yun Lee, Jhang Ho Pak, Woon-Mok Sohn, Sung-Jong Hong, John Pius Dalton
PLOS Neglected Tropical Diseases.2018; 12(4): e0006459. CrossRef
Molecular and structural characteristics of multidrug resistance-associated protein 7 in Chinese liver fluke Clonorchis sinensis
Fuhong Dai, Won Gi Yoo, Ji-Yun Lee, Yanyan Lu, Jhang Ho Pak, Woon-Mok Sohn, Sung-Jong Hong
Parasitology Research.2017; 116(3): 953. CrossRef

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Mini Review

Functional Genes and Proteins of Clonorchis sinensis: Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong; Korean J Parasitol 2009;47(Suppl):S59.
Published online October 27, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.S.S59

Abstract
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During the past several decades, researches on parasite genetics have progressed from biochemical and serodiagnostic studies to protein chemistry, molecular biology, and functional gene studies. Nowadays, bioinformatics, genomics, and proteomics approaches are being applied by Korean parasitology researchers. As for Clonorchis sinensis, investigations have been carried out to identify its functional genes using forward and reverse genetic approaches and to characterize the biochemical and biological properties of its gene products. The authors review the proteins of cloned genes, which include antigenic proteins, physiologic and metabolic enzymes, and the gene expression profile of Clonorchis sinensis.

Citations

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Clonorchis sinensis ESPs enhance the activation of hepatic stellate cells by a cross-talk of TLR4 and TGF-β/Smads signaling pathway
Bo Li, Chao Yan, Jing Wu, Koda Stephane, Xin Dong, Yu-Zhao Zhang, Yu Zhang, Qian Yu, Kui-Yang Zheng
Acta Tropica.2020; 205: 105307. CrossRef
Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics
Pyo Yun Cho, Ji-Yun Lee, Tae Im Kim, Jin-Ho Song, Sung-Jong Hong, Won Gi Yoo, Takafumi Tsuboi, Kwon-Soo Ha, Jae-Wan Jung, Satoru Takeo, Eun-Taek Han, Banchob Sripa, Sung-Tae Hong, Jong-Yil Chai, Ho-Woo Nam, Jhang Ho Pak, Tong-Soo Kim, Krystyna Cwiklinski
PLOS Neglected Tropical Diseases.2020; 14(12): e0008998. CrossRef
Clonorchiasis
Men-Bao Qian, Jürg Utzinger, Jennifer Keiser, Xiao-Nong Zhou
The Lancet.2016; 387(10020): 800. CrossRef
Current status and perspectives of Clonorchis sinensis and clonorchiasis: epidemiology, pathogenesis, omics, prevention and control
Ze-Li Tang, Yan Huang, Xin-Bing Yu
Infectious Diseases of Poverty.2016;[Epub] CrossRef
Clonorchis sinensis, an oriental liver fluke, as a human biological agent of cholangiocarcinoma: a brief review
Tong-Soo Kim, Jhang Ho Pak, Jong-Bo Kim, Young Yil Bahk
BMB Reports.2016; 49(11): 590. CrossRef
Clonorchis sinensis acetoacetyl-CoA thiolase: identification and characterization of its potential role in surviving in the bile duct
Jinsi Lin, Hongling Qu, Guishan Chen, Lei He, Yanquan Xu, Zhizhi Xie, Mengyu Ren, Jiufeng Sun, Shan Li, Wenjun Chen, Xueqing Chen, Xiaoyun Wang, Xuerong Li, Chi Liang, Yan Huang, Xinbing Yu
Parasites & Vectors.2015;[Epub] CrossRef
Clonorchis sinensis ferritin heavy chain triggers free radicals and mediates inflammation signaling in human hepatic stellate cells
Qiang Mao, Zhizhi Xie, Xiaoyun Wang, Wenjun Chen, Mengyu Ren, Mei Shang, Huali Lei, Yanli Tian, Shan Li, Pei Liang, Tingjin Chen, Chi Liang, Jin Xu, Xuerong Li, Yan Huang, Xinbing Yu
Parasitology Research.2015; 114(2): 659. CrossRef
Biochemical and immunological characterization of annexin B30 from Clonorchis sinensis excretory/secretory products
Lei He, Mengyu Ren, Xueqing Chen, Xiaoyun Wang, Shan Li, Jinsi Lin, Chi Liang, Pei Liang, Yue Hu, Huali Lei, Meng Bian, Yan Huang, Zhongdao Wu, Xuerong Li, Xinbing Yu
Parasitology Research.2014; 113(7): 2743. CrossRef
Identification, immunolocalization, and characterization analyses of an exopeptidase of papain superfamily, (cathepsin C) from Clonorchis sinensis
Pei Liang, Lei He, Yanquan Xu, Xueqing Chen, Yan Huang, Mengyu Ren, Chi Liang, Xuerong Li, Jin Xu, Gang Lu, Xinbing Yu
Parasitology Research.2014; 113(10): 3621. CrossRef
Proteomic identification of potential Clonorchis sinensis excretory/secretory products capable of binding and activating human hepatic stellate cells
Xiaoyun Wang, Fengyu Hu, Xuchu Hu, Wenjun Chen, Yan Huang, Xinbing Yu
Parasitology Research.2014; 113(8): 3063. CrossRef
Characterization of the secreted cathepsin B cysteine proteases family of the carcinogenic liver fluke Clonorchis sinensis
Wenjun Chen, Xiaoyun Wang, Xiaoli Lv, Yanli Tian, Yanquan Xu, Qiang Mao, Mei Shang, Xuerong Li, Yan Huang, Xinbing Yu
Parasitology Research.2014; 113(9): 3409. CrossRef
ClonorESTdb: a comprehensive database for Clonorchis sinensis EST sequences
Dae-Won Kim, Won Gi Yoo, Sanghyun Lee, Myoung-Ro Lee, Yu-Jung Kim, Shin-Hyeong Cho, Won-Ja Lee, Jung-Won Ju
BMC Research Notes.2014;[Epub] CrossRef
Adult Opisthorchis felineus major protein fractions deduced from transcripts: Comparison with liver flukes Opisthorchis viverrini and Clonorchis sinensis
Mikhail Pomaznoy, Sergey Tatkov, Alexey Katokhin, Dmitry Afonnikov, Vladimir Babenko, Dagmara Furman, Ilya Brusentsov, Pavel Belavin, Alexandr Najakshin, Sergey Guselnikov, Gennady Vasiliev, Anton Sivkov, Egor Prokhortchouk, Konstantin Skryabin, Viatchesl
Experimental Parasitology.2013; 135(2): 297. CrossRef
Biochemical characterization and functional analysis of fructose-1,6-bisphosphatase from Clonorchis sinensis
Pei Liang, Jiufeng Sun, Yan Huang, Fan Zhang, Juanjuan Zhou, Yue Hu, Xiaoyun Wang, Chi Liang, Minghui Zheng, Yanquan Xu, Qiang Mao, Xuchu Hu, Xuerong Li, Jin Xu, Gang Lu, Xinbing Yu
Molecular Biology Reports.2013; 40(7): 4371. CrossRef
Genetic diversity of nuclear ITS1–5.8S–ITS2 rDNA sequence in Clonorchis sinensis Cobbold, 1875 (Trematoda: Opisthorchidae) from the Russian Far East
Yulia V. Tatonova, Galina N. Chelomina, Vladimir V. Besprosvannykh
Parasitology International.2012; 61(4): 664. CrossRef
Genomics and molecular genetics of Clonorchis sinensis: Current status and perspectives
Si-Yang Huang, Guang-Hui Zhao, Bao-Quan Fu, Min-Jun Xu, Chun-Ren Wang, Song-Ming Wu, Feng-Cai Zou, Xing-Quan Zhu
Parasitology International.2012; 61(1): 71. CrossRef
Molecular characterization of Clonorchis sinensis tetraspanin 2 extracellular loop 2
Tae Yun Kim, Eun Joo Chung, Woon-Mok Sohn, Sung-Hong Hong, Tai-Soon Yong
Parasitology Research.2012; 110(2): 707. CrossRef
Developmental Transcriptomic Features of the Carcinogenic Liver Fluke, Clonorchis sinensis
Won Gi Yoo, Dae-Won Kim, Jung-Won Ju, Pyo Yun Cho, Tae Im Kim, Shin-Hyeong Cho, Sang-Haeng Choi, Hong-Seog Park, Tong-Soo Kim, Sung-Jong Hong, Banchob Sripa
PLoS Neglected Tropical Diseases.2011; 5(6): e1208. CrossRef
Fish-borne Parasitic Diseases
Jong-Yil Chai
Hanyang Medical Reviews.2010; 30(3): 223. CrossRef

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Brief Communication

Metacercarial proteins interacting with WD40-repeat protein of Clonorchis sinensis: Pyo Yun Cho, Tae Im Kim, Shunyu Li, Sung-Jong Hong, Min-Ho Choi, Sung-Tae Hong, Yong Je Chung; Korean J Parasitol 2007;45(3):229-232.
Published online September 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.3.229

Abstract
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The WD40-repeat proteins serve as a platform coordinating partner proteins and are involved in a range of regulatory cellular functions. A WD40-repeat protein (CsWD1) of Clonorchis sinensis previously cloned is expressed stage-specifically in the tegumental syncytium of C. sinensis metacercariae. In the present study, interacting proteins with the CsWD1 protein was purified by immunoprecipitation and 2 dimension gel electrophoresis from the C. sinensis metacercaria soluble extract, and tryptic peptides were analyzed by LC/ESI-MS. Putative partner proteins were annotated to be actin-2, glyceraldehyde-3-phosphate dehydrogenase, and hypothetical and unmanned proteins. The CsWD1 protein was predicted to contain 3 conserved actin-interacting residues on its functional surface. With these results, the CsWD1 protein is suggested to be an actin-interacting protein of C. sinensis.

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