Echinococcus granulosus sensu lato (s.l.) is a causative agent of cystic echinococcosis or cystic hydatid disease in humans and domestic and wild animals. The disease is a serious health problem in countries associated with poverty and poor hygiene practices, particularly in livestock raising. We introduced a practical algorism for genotyping the parasite, which may be useful to many developing countries. To evaluate the efficiency of the algorism, we genotyped 3 unknown strains isolated from human patients. We found that unknowns 1 and 3 were included in G1, G2, and G3 genotypes group and unknown 2 was included in G4 genotype (Echinococcus equinus) according to the algorisms. We confirmed
these results by sequencing the 3 unknown isolates cox1 and nad1 PCR products. In conclusion, these new algorisms are very fast genotype identification tools that are suitable for evaluating E. granulosus s.l. isolated from livestock or livestock holders, particularly in developing countries.
Citations
Citations to this article as recorded by
Primary cerebral cystic echinococcosis in a child from Roman countryside: Source attribution and scoping review of cases from the literature Adriano Casulli, Stefania Pane, Franco Randi, Paola Scaramozzino, Andrea Carvelli, Carlo Efisio Marras, Andrea Carai, Azzurra Santoro, Federica Santolamazza, Francesca Tamarozzi, Lorenza Putignani, Solange Bresson-Hadni PLOS Neglected Tropical Diseases.2023; 17(9): e0011612. CrossRef
A validated method to identify Echinococcus granulosus sensu lato at species level F. Santolamazza, A. Santoro, A. Possenti, S.M. Cacciò, A. Casulli Infection, Genetics and Evolution.2020; 85: 104575. CrossRef
A pilot comparative study between serological and genetic investigations in relationship to clinical outcomes on patients with cystic echinococcosis Nu. Altintas, S. A. Karamil, O. Turkum, M. Akil, A. Sakarya, H. Bozkaya, J. Šoltys, Na. Altintas Helminthologia.2020; 57(2): 91. CrossRef
Evaluation of PCR‐HRM, RFLP, and direct sequencing as simple and cost‐effective methods to detect common EGFR mutations in plasma cell–free DNA of non–small cell lung cancer patients Jamal Zaini, Elisna Syahruddin, Muhammad Yunus, Sita Laksmi Andarini, Achmad Hudoyo, Najmiatul Masykura, Refniwita Yasril, Asep Ridwanuloh, Heriawaty Hidajat, Fariz Nurwidya, Sony Suharsono, Ahmad R.H. Utomo Cancer Reports.2019;[Epub] CrossRef
First
Prev
