Parasites, Hosts and Diseases

"Tiantian Zhang"

Brief Communications

Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae): Tiantian Zhang, Xuejiao Cui, Jincheng Zhang, Hui Wang, Meng Wu, Hua Zeng, Yuanyuan Cao, Jingze Liu, Yonghong Hu; Korean J Parasitol 2015;53(6):789-793.
Published online December 31, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.6.789

Abstract
PDF

In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum.

Citations

Citations to this article as recorded by

Characterization of tick salivary gland and saliva alphagalactome reveals candidate alpha-gal syndrome disease biomarkers
Margarita Villar, Iván Pacheco, Lourdes Mateos-Hernández, Alejandro Cabezas-Cruz, Ala E. Tabor, Manuel Rodríguez-Valle, Albert Mulenga, Katherine M. Kocan, Edmour F. Blouin, José de La Fuente
Expert Review of Proteomics.2021; 18(12): 1099. CrossRef
Extracellular vesicles induce protective immunity against Trichuris muris
R. K. Shears, A. J. Bancroft, G. W. Hughes, R. K. Grencis, D. J. Thornton
Parasite Immunology.2018;[Epub] CrossRef
New approaches and omics tools for mining of vaccine candidates against vector-borne diseases
Josipa Kuleš, Anita Horvatić, Nicolas Guillemin, Asier Galan, Vladimir Mrljak, Mangesh Bhide
Molecular BioSystems.2016; 12(9): 2680. CrossRef

9,219 View
89 Download
5 Web of Science
Crossref

Gene Cloning, Expression and Immunogenicity of the Protective Antigen Subolesin in Dermacentor silvarum: Yonghong Hu, Hua Zeng, Jincheng Zhang, Duo Wang, Dongming Li, Tiantian Zhang, Shujie Yang, Jingze Liu; Korean J Parasitol 2014;52(1):93-97.
Published online February 19, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.1.93

Abstract
PDF

Subolesin (4D8), the ortholog of insect akirins, is a highly conserved protective antigen and thus has the potential for development of a broad-spectrum vaccine against ticks and mosquitoes. To date, no protective antigens have been characterized nor tested as candidate vaccines against Dermacentor silvarum bites and transmission of associated pathogens. In this study, we cloned the open reading frame (ORF) of D. silvarum 4D8 cDNA (Ds4D8), which consisted of 498 bp encoding 165 amino acid residues. The results of sequence alignments and phylogenetic analysis demonstrated that D. silvarum 4D8 (Ds4D8) is highly conserved showing more than 81% identity of amino acid sequences with those of other hard ticks. Additionally, Ds4D8 containing restriction sites was ligated into the pET-32(a+) expression vector and the recombinant plasmid was transformed into Escherichia coli rosetta. The recombinant Ds4D8 (rDs4D8) was induced by isopropyl β-D-thiogalactopyranoside (IPTG) and purified using Ni affinity chromatography. The SDS-PAGE results showed that the molecular weight of rDs4D8 was 40 kDa, which was consistent with the expected molecular mass considering 22 kDa histidine-tagged thioredoxin (TRX) protein from the expression vector. Western blot results showed that rabbit anti-D. silvarum serum recognized the expressed rDs4D8, suggesting an immune response against rDs4D8. These results provided the basis for developing a candidate vaccine against D. silvarum ticks and transmission of associated pathogens.

Citations

Citations to this article as recorded by

Subolesin gene structure and mRNA isoform diversity in South African R. microplus ticks: Relevance for understanding subolesin-based tick vaccines
Elsje Christine Rabie, Christine Maritz-Olivier
Ticks and Tick-borne Diseases.2025; 16(4): 102502. CrossRef
Transmission-Blocking Vaccines: Focus on Anti-Vector Vaccines against Tick-Borne Diseases
Girish Neelakanta, Hameeda Sultana
Archivum Immunologiae et Therapiae Experimentalis.2015; 63(3): 169. CrossRef
Screening and Identification of Antigenic Proteins from the Hard Tick <i>Dermacentor silvarum</i> (Acari: Ixodidae)
Tiantian Zhang, Xuejiao Cui, Jincheng Zhang, Hui Wang, Meng Wu, Hua Zeng, Yuanyuan Cao, Jingze Liu, Yonghong Hu
The Korean Journal of Parasitology.2015; 53(6): 789. CrossRef

9,284 View
95 Download
3 Web of Science
Crossref

Original Article

Screening and Molecular Cloning of a Protective Antigen from the Midgut of Haemaphysalis longicornis: Yonghong Hu, Jincheng Zhang, Shujie Yang, Hui Wang, Hua Zeng, Tiantian Zhang, Jingze Liu; Korean J Parasitol 2013;51(3):327-334.
Published online June 30, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.3.327

Abstract
PDF

Vaccination is considered a promising alternative for controlling tick infestations. Haemaphysalis longicornis midgut proteins separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane were screened for protective value against bites. The western blot demonstrated the immunogenicity of 92 kDa protein (P92). The analysis of the P92 amino acid sequence by LC-MS/MS indicated that it was a H. longicornis paramyosin (Hl-Pmy). The full lenghth cDNA of Hl-Pmy was obtained by rapid amplification of cDNA ends (RACE) which consisted of 2,783 bp with a 161 bp 3' untranslated region. Sequence alignment of tick paramyosin (Pmy) showed that Hl-Pmy shared a high level of conservation among ticks. Comparison with the protective epitope sequence of other invertebrate Pmy, it was calculated that the protective epitope of Hl-Pmy was a peptide (LEEAEGSSETVVEMNKKRDTE) named LEE, which was close to the N-terminal of Hl-Pmy protein. The secondary structure analysis suggested that LEE had non-helical segments within an α-helical structure. These results provide the basis for developing a vaccine against biting H. longicornis ticks.

Citations

Citations to this article as recorded by

The current strategies and underlying mechanisms in the control of the vector tick, Haemaphysalis longicornis: Implications for future integrated management
Chuks F. Nwanade, Min Wang, Sisi Li, Zhijun Yu, Jingze Liu
Ticks and Tick-borne Diseases.2022; 13(2): 101905. CrossRef
Evaluation on two types of paramyosin vaccines for the control of Haemaphysalis longicornis infestations in rabbits
Pin-Xing Wu, Xue-Jiao Cui, Mi-Xue Cao, Li-Hong Lv, Hong-Meng Dong, Shu-Wen Xiao, Jing-Ze Liu, Yong-Hong Hu
Parasites & Vectors.2021;[Epub] CrossRef
Morphology and ultrastructure changes of Haemaphysalis longicornis Neumann (Acari: Ixodidae) female adult ticks at different developmental stages
Fang Wang, Duo Wang, Chao-Zhong Jiang, Na Liang, Yong-Hong Hu, Jing-Ze Liu
International Journal of Acarology.2017; 43(4): 308. CrossRef
Evaluation of immune protection induced by DNA vaccines from Haemaphysalis longicornis paramyosin in rabbits
Tian-Tian Zhang, Jin-Cheng Zhang, Xue-Jiao Cui, Jing-Jing Zheng, Ru Li, Fang Wang, Jing-Ze Liu, Yong-Hong Hu
Parasites & Vectors.2017;[Epub] CrossRef
Paramyosin of canine Onchocerca lupi: usefulness for the diagnosis of a neglected zoonotic disease
Bronwyn Campbell, Helder Cortes, Giada Annoscia, Alessio Giannelli, Antonio Parisi, Maria Stefania Latrofa, Filipe Dantas-Torres, Luís Cardoso, Domenico Otranto
Parasites & Vectors.2016;[Epub] CrossRef
Screening and Identification of Antigenic Proteins from the Hard Tick <i>Dermacentor silvarum</i> (Acari: Ixodidae)
Tiantian Zhang, Xuejiao Cui, Jincheng Zhang, Hui Wang, Meng Wu, Hua Zeng, Yuanyuan Cao, Jingze Liu, Yonghong Hu
The Korean Journal of Parasitology.2015; 53(6): 789. CrossRef