Allyl isothiocyanate (AITC) is a natural product commonly used in food preservation and pharmaceutical applications. Toxoplasmosis, caused by the protozoan pathogen Toxoplasma gondii, is prevalent globally while the impact of AITC on toxoplasmosis is unclear. We explored the effect of AITC on acute toxoplasmosis. We infected C57BL/6 mice with T. gondii type I RH strain following AITC administration. On the 4th day after infection, which corresponds to the initial stage of infection, we collected serum for the determination of inflammatory cytokine levels. The mice serum of the AITC-administered group contained significantly lower levels of granulocyte colony-stimulating factor, interferon-gamma, interleukin (IL)-23 subunit p19, IL-4, IL-6, and monocyte chemoattractant protein-1. The lifespan of the mice in the AITC-administered group was significantly reduced. In vitro experiments showed that AITC promoted the proliferation of intracellular T. gondii accompanied by the inhibition of IL-4, IL-1β, and IL-6 production in RAW264.7 macrophages. Our results showed that AITC facilitated T. gondii infection in the early stage by inhibiting the production of several inflammatory cytokines.
Alexandre Alberto Tonin, Aleksandro Schafer da Silva, Maria Luiza Thorstenberg, L?via Gelain Castilhos, Raqueli Teresinha Fran?a, Daniela Bitencourt Rosa Leal, Marta Maria Medeiros Frescura Duarte, Fernanda Silveira Flores Vogel, Mario Luiz de La Rue, Sonia Terezinha dos Anjos Lopes
Korean J Parasitol 2013;51(4):421-426. Published online August 30, 2013
Several studies have shown the mechanisms and importance of immune responses against Toxoplasma gondii infection and the notable role of cholinesterases in inflammatory reactions. However, the association between those factors has not yet been investigated. Therefore, the aim of this study was to evaluate the acetylcholinesterase (AChE) activity in blood and lymphocytes and the activity of butyrylcholinesterase (BChE) in serum of rats experimentally infected with T. gondii during the acute phase of infection. For that, an in vivo study was performed with evaluations of AChE and BChE activities on days 5 and 10 post-infection (PI). The activity of AChE in blood was increased on day 5 PI, while in lymphocytes its activity was enhanced on days 5 and 10 PI (P<0.05). No significant difference was observed between groups regarding to the activity of BChE in serum. A positive (P<0.01) correlation was observed between AChE activity and number of lymphocytes. The role of AChE as an inflammatory marker is well known in different pathologies; thus, our results lead to the hypothesis that AChE has an important role in modulation of early immune responses against T. gondii infection.
Citations
Citations to this article as recorded by
A meta-analysis of changes in cholinesterase activity among experimental animals infected with Toxoplasma gondii F. K. Mohammad, S. H. Eassa, R. G. Abdullah BULGARIAN JOURNAL OF VETERINARY MEDICINE.2025; 28(2): 195. CrossRef
Variation in Gene Expression Across Infection Status and Elevation in a Hawaiian Honeycreeper Loren Cassin‐Sackett, Katherine M. McClure, Taylor E. Callicrate, Eben H. Paxton, Robert C. Fleischer Ecology and Evolution.2025;[Epub] CrossRef
Plasma Cholinesterase Activity in Patients With Rheumatoid Arthritis and Toxoplasmosis Rabie G Abdullah, Souzan H Eassa, Fouad K Mohammad Cureus.2023;[Epub] CrossRef
Toxoplasmosis: Targeting neurotransmitter systems in psychiatric disorders Tooran Nayeri, Shahabeddin Sarvi, Ahmad Daryani Metabolic Brain Disease.2022; 37(1): 123. CrossRef
Toxoplasma gondii: A possible etiologic agent for Alzheimer's disease Tooran Nayeri, Shahabeddin Sarvi, Mehdi Sharif, Ahmad Daryani Heliyon.2021; 7(6): e07151. CrossRef
Diagnostic significance of C-reactive protein and hematological parameters in acute toxoplasmosis Vanessa Sandri, Itamar Luís Gonçalves, Gustavo Machado das Neves, Marisa Lucia Romani Paraboni Journal of Parasitic Diseases.2020; 44(4): 785. CrossRef
STUDY ON THE PREVALENCE AND HEMATOLOGICAL ALTERATIONS IN TOXOPLASMA GONDII INFECTED CAPTIVE PHEASANT SPECIES OF BAHAWALPUR ZOO, PAKISTAN M.H. Lashari, M Bibi, U Farooq, F Afzal, A Ali, M Safdar, M.S. Akhtar, A.A. Farooq, S Masood, M Ayaz, M.I. Khan The Journal of Animal and Plant Sciences.2020; 31(2): 625. CrossRef
Is There a Cholinergic Survival Incentive for Neurotropic Parasites in the Brain? Abdul Mannan Baig ACS Chemical Neuroscience.2017; 8(12): 2574. CrossRef
Sulfamethoxazole-trimethoprim associated with resveratrol for the treatment of toxoplasmosis in mice: Influence on the activity of enzymes involved in brain neurotransmission Nathieli B. Bottari, Matheus D. Baldissera, Alexandre A. Tonin, Virginia C. Rech, Vivian S.K. Nishihira, Gustavo R. Thomé, Maria Rosa C. Schetinger, Vera M. Morsch, Giovana Camillo, Fernanda F. Vogel, Camila Tochetto, Rafael Fighera, Gustavo Machado, Leni Microbial Pathogenesis.2015; 79: 17. CrossRef
First
Prev
