Parasites, Hosts and Diseases

"excretory/secretory proteins"

Brief Communication

Molecular cloning of a rhoptry protein (ROP6) secreted from Toxoplasma gondii: Hye-Jin Ahn, Sehra Kim, Ho-Woo Nam; Korean J Parasitol 2006;44(3):251-254.
Published online September 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.3.251

Abstract
PDF

Monoclonal antibody (mAb) Tg786 against Toxoplasma gondii has been found to detect a 42-kDa rhoptry protein (ROP6) which showed protease activity and host cell binding characteristics after secretion. Using the mAb, a colony containing a 3'-UTR was probed in a T. gondii cDNA expression library. A full length cDNA sequence of the rhoptry protein was completed after 5'-RACE, which consisted of 1,908 bp with a 1,443 bp ORF. The deduced amino acid sequence of ROP6 consisted of a polypeptide of 480 amino acids without significant homology to any other known proteins. This sequence contains an amino terminal stop transfer sequence downstream of a short neutral sequence, hydrophilic middle sequence, and hydrophobic carboxy terminus. It is suggested that the ROP6 is inserted into the rhoptry membrane with both N- and C-termini.

Citations

Citations to this article as recorded by

A systematic review of Toxoplasma gondii antigens to find the best vaccine candidates for immunization
Fatemeh Rezaei, Shahabeddin Sarvi, Mahdi Sharif, Seyed Hossein Hejazi, Abdol sattar Pagheh, Sargis A. Aghayan, Ahmad Daryani
Microbial Pathogenesis.2019; 126: 172. CrossRef
Toxoplasma gondii Secretory Proteins and their Role in Invasion and Pathogenesis
Yang Zhang, Bo Shiun Lai, Mario Juhas, Yun Zhang
Microbiological Research.2019;[Epub] CrossRef
A systematic review on the role of GRA proteins of Toxoplasma gondii in host immunization
Fatemeh Rezaei, Mahdi Sharif, Shahabeddin Sarvi, Seyed Hossein Hejazi, Sargis Aghayan, Abdol Sattar Pagheh, Samira Dodangeh, Ahmad Daryani
Journal of Microbiological Methods.2019; 165: 105696. CrossRef
Rhoptry protein 6 from Toxoplasma gondii is an intrinsically disordered protein
Won-Kyu Lee, Hye-Jin Ahn, Yeon Gyu Yu, Ho-Woo Nam
Protein Expression and Purification.2014; 101: 146. CrossRef
Electrophoretic Patterns of Toxoplasma gondii Excreted/Secreted Antigens and Their Role in Induction of the Humoral Immune Response
Ahmad Daryani, Mehdi Sharif, Hamed Kalani, Alireza Rafiei, Farzad Kalani, Ehsan Ahmadpour
Jundishapur Journal of Microbiology.2014;[Epub] CrossRef
Kiss and spit: the dual roles of Toxoplasma rhoptries
John C. Boothroyd, Jean-Francois Dubremetz
Nature Reviews Microbiology.2008; 6(1): 79. CrossRef
Characterization of a New Gene <italic>wx2</italic> in <italic>Toxoplasma gondii</italic>
Xiang Wu, Qiong Zhang, Kui Tan, Ronghua Xie, Jiubo Fan, Hengping Shu, Shiping Wang
Acta Biochimica et Biophysica Sinica.2007; 39(7): 475. CrossRef

7,419 View
52 Download
Crossref

Original Articles

Protease activity of 80 kDa protein secreted from the apicomplexan parasite Toxoplasma gondii: Kyoung-Ju Song, Ho-Woo Nam; Korean J Parasitol 2003;41(3):165-169.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.165

Abstract
PDF

This study describes the characterization of 80 kDa protease showing gelationlytic property among three proteases in the excretory/secretory proteins (ESP) from Toxoplasma gondii. The protease activity was detected in the ESP but not in the somatic extract of RH tachyzoites. This protease was active only in the presence of calcium ion but not other divalent cationic ions such as Cu²⁺, Zn²⁺, Mg²⁺, and Mn²⁺, implying that Ca²⁺ is critical factor for the activation of the protease. The 80 kDa protease was optimally active at pH 7.5. Its gelatinolytic activity was maximal at 37℃, and significant level of enzyme activity of the protease remained after heat treatment at 56℃ for 30 min or 100℃ for 10 min. This thermostable enzyme was strongly inhibited by metal chelators, i.e., EDTA, EGTA, and 1,10-phenanthroline. Thus, the 80 kDa protease n the ESP secreted by T. gondii was classified as a calcium dependent neutral metalloprotease.

Citations

Citations to this article as recorded by

Purification and characterization of cysteine protease of Sarcocystis fusiformis from infected Egyptian water buffaloes
Amal Z. Barakat, Azza M. Abdel-Aty, Marwa K. Ibrahim, Hala A. Salah, Usama M. Hegazy, Rasha A. M. Azouz, Roqaya I. Bassuiny, Raafat M. Shaapan, Saleh A. Mohamed
Scientific Reports.2023;[Epub] CrossRef
In vitro activity of N-phenyl-1,10-phenanthroline-2-amines against tachyzoites and bradyzoites of Toxoplasma gondii
Erica S. Martins-Duarte, Juliana de Araujo Portes, Ramon Borges da Silva, Hugo Silva Pires, Simon J. Garden, Wanderley de Souza
Bioorganic & Medicinal Chemistry.2021; 50: 116467. CrossRef
Characterization of metalloproteases and serine proteases of Toxoplasma gondii tachyzoites and their effect on epithelial cells
Carlos J. Ramírez-Flores, Rosalba Cruz-Mirón, Rossana Arroyo, Mónica E. Mondragón-Castelán, Tais Nopal-Guerrero, Sirenia González-Pozos, Emmanuel Ríos-Castro, Ricardo Mondragón-Flores
Parasitology Research.2019; 118(1): 289. CrossRef
Host-Toxoplasma gondii Coadaptation Leads to Fine Tuning of the Immune Response
Thaís Rigueti Brasil, Celio Geraldo Freire-de-Lima, Alexandre Morrot, Andrea Cristina Vetö Arnholdt
Frontiers in Immunology.2017;[Epub] CrossRef
Toxoplasma gondii: Effects of neuwiedase, a metalloproteinase from Bothrops neuwiedi snake venom, on the invasion and replication of human fibroblasts in vitro
Luciana Machado Bastos, Robson J. Oliveira Júnior, Deise Aparecida Oliveira Silva, José Roberto Mineo, Carlos Ueira Vieira, David Nascimento Silva Teixeira, Maria Inês Homsi-Brandeburgo, Veridiana Melo Rodrigues, Amélia Hamaguchi
Experimental Parasitology.2008; 120(4): 391. CrossRef

8,609 View
68 Download
Crossref

Detection and characterization of excretory/secretory proteins from Toxoplasma gondii by monoclonal antibodies: Eui-Sun Son, Ho-Woo Nam; Korean J Parasitol 2001;39(1):49-56.
Published online March 31, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.1.49

Abstract
PDF

Excretory/secretory proteins (ESP) from Toxoplasma gondii were analyzed to define the function in the penetration process into host cells. Whole ESP obtained at 37℃ were composed of 15 bands with molecular mass of 110, 97, 86, 80, 70, 60, 54, 42, 40, 36, 30, 28, 26, 22, and 19 kDa. Five ESP of 86, 80, 42, 36, and 28 kDa were reacted with monoclonal antibodies (mAb), named as Tg386 (microneme), Tg485 (surface membrane), Tg786 (rhoptry), Tg378, and Tg556 (both dense granules), respectively. The ESP was released by a temperature-dependent/-independent manner and all at once whenever ready to pour out except Tg786. Each ESP was not exhausted within the parasite but the amount was limited. Tg786 was released continuously with increment, whereas Tg378 and Tg556 were ceased to release after 3 and 4 hr. Dense granular Tg378 and Tg556 were released spontaneously and constitutively before the entry into host cells also. The entry of T. gondii was inhibited by all the mAbs differentially. And the parasite deprived of ESP was inhibited to enter exponentially up to 90.1%. It is suggested that ESP play an essential function to provide appropriate environment for the entry of the parasite into host cells.

Citations

Citations to this article as recorded by

T. gondii excretory proteins promote the osteogenic differentiation of human bone mesenchymal stem cells via the BMP/Smad signaling pathway
Mingzhu Deng, Feifei Gao, Tianfeng Liu, Weiqiang Zhan, Juanhua Quan, Ziquan Zhao, Xuyang Wu, Zhuolan Zhong, Hong Zheng, Jiaqi Chu
Journal of Orthopaedic Surgery and Research.2024;[Epub] CrossRef
FAF1 downregulation by Toxoplasma gondii enables host IRF3 mobilization and promotes parasite growth
Fei‐Fei Gao, Juan‐Hua Quan, In‐Wook Choi, Yeon‐Jae Lee, Seul‐Gi Jang, Jae‐Min Yuk, Young‐Ha Lee, Guang‐Ho Cha
Journal of Cellular and Molecular Medicine.2021; 25(19): 9460. CrossRef
Toxoplasma gondii Induces Apoptosis via Endoplasmic Reticulum Stress-Derived Mitochondrial Pathway in Human Small Intestinal Epithelial Cell-Line
Hao Wang, Chunchao Li, Wei Ye, Zhaobin Pan, Jinhui Sun, Mingzhu Deng, Weiqiang Zhan, Jiaqi Chu
The Korean Journal of Parasitology.2021; 59(6): 573. CrossRef
FOXO1-Mediated NOX4 Expression is Required for Host to Defense in the Model of Ocular Toxoplasmosis
Wei Zhou, Fei-Fei Gao, Jang Ho Lee, Im-Soon Lee, Jae-Min Yuk, Kyoung Sang Cho, Young-Ha Lee, Guang-Ho Cha
SSRN Electronic Journal .2021;[Epub] CrossRef
The Role of PI3K/AKT Pathway and NADPH Oxidase 4 in Host ROS Manipulation by Toxoplasma gondii
Hei Gwon Choi, Fei-Fei Gao, Wei Zhou, Pu-Reum Sun, Jae-Min Yuk, Young-Ha Lee, Guang-Ho Cha
The Korean Journal of Parasitology.2020; 58(3): 237. CrossRef
Evaluation of Toxoplasma gondii soluble, whole and excretory/secretary antigens for diagnosis of toxoplasmosis by ELISA test
S. Pishkari, S. Shojaee, H. Keshavarz, M. Salimi, M. Mohebali
Journal of Parasitic Diseases.2017; 41(1): 289. CrossRef
A Human Proteome Array Approach to Identifying Key Host Proteins Targeted by Toxoplasma Kinase ROP18
Zhaoshou Yang, Yongheng Hou, Taofang Hao, Hee-Sool Rho, Jun Wan, Yizhao Luan, Xin Gao, Jianping Yao, Aihua Pan, Zhi Xie, Jiang Qian, Wanqin Liao, Heng Zhu, Xingwang Zhou
Molecular & Cellular Proteomics.2017; 16(3): 469. CrossRef
Investigation of Toxoplasma gondii Infection in Cutaneous Leishmaniasis Patients of the Isfahan Province
Abbasali Eskandarian, Somayeh Jahani, Hossein Hejazi, Hossein Yousefi, Vahid Raissi
International Journal of Infection.2016;[Epub] CrossRef
Investigation of Toxoplasma gondii Infection in Cutaneous Leishmaniasis Patients of the Isfahan Province
Abbasali Eskandarian, Somayeh Jahani, Hossein Hejazi, Hossein Yousefi, Vahid Raissi
International Journal of Infection.2016;[Epub] CrossRef
Electrophoretic Patterns of Toxoplasma gondii Excreted/Secreted Antigens and Their Role in Induction of the Humoral Immune Response
Ahmad Daryani, Mehdi Sharif, Hamed Kalani, Alireza Rafiei, Farzad Kalani, Ehsan Ahmadpour
Jundishapur Journal of Microbiology.2014;[Epub] CrossRef
Rhoptry protein 6 from Toxoplasma gondii is an intrinsically disordered protein
Won-Kyu Lee, Hye-Jin Ahn, Yeon Gyu Yu, Ho-Woo Nam
Protein Expression and Purification.2014; 101: 146. CrossRef
Comprehensive Proteome Analysis of the Excretory/Secretory Proteins of Toxoplasma gondii
Won-Kyu Lee, Hye-Jin Ahn, Je-Hyun Baek, Chong-Heon Lee, Yeon Gyu Yu, Ho-Woo Nam
Bulletin of the Korean Chemical Society.2014; 35(10): 3071. CrossRef
High Expression of Water-Soluble Recombinant Antigenic Domains ofToxoplasma gondii Secretory Organelles
Zhaoshou Yang, Hye-Jin Ahn, Ho-Woo Nam
The Korean Journal of Parasitology.2014; 52(4): 367. CrossRef
Heat Shock Protein 90 of Bonamia ostreae: Characterization and Possible Correlation with Infection of the Flat Oyster, Ostrea edulis
Maria Prado‐Alvarez, Bruno Chollet, Yann Couraleau, Benjamin Morga, Isabelle Arzul
Journal of Eukaryotic Microbiology.2013; 60(3): 257. CrossRef
Kinetics of IL-23 and IL-12 Secretion in Response to Toxoplasma gondii Antigens from THP-1 Monocytic Cells
Juan-Hua Quan, Wei Zhou, Guang-Ho Cha, In-Wook Choi, Dae-Whan Shin, Young-Ha Lee
The Korean Journal of Parasitology.2013; 51(1): 85. CrossRef
Toxoplasma gondii Proliferation Require Down-Regulation of Host Nox4 Expression via Activation of PI3 Kinase/Akt Signaling Pathway
Wei Zhou, Juan-Hua Quan, Young-Ha Lee, Dae-Whan Shin, Guang-Ho Cha, Ira J. Blader
PLoS ONE.2013; 8(6): e66306. CrossRef
Evaluation of Excreted/Secreted Antigens Derived from Peritoneal of Toxoplasma Infected Small Mice to Detect IgG Against Toxoplasma
Seyed Hossein Abdollahi, Mohammad Kazemi Arababadi, Gholamhossein Hassanshah
Pakistan Journal of Biological Sciences.2009; 12(6): 530. CrossRef
Interaction between Parasitophorous Vacuolar Membrane-associated GRA3 and Calcium Modulating Ligand of Host Cell Endoplasmic Reticulum in the Parasitism of Toxoplasma gondii
Ji Yeon Kim, Hye-Jin Ahn, Kyung Ju Ryu, Ho-Woo Nam
The Korean Journal of Parasitology.2008; 46(4): 209. CrossRef
Host cell binding of GRA10, a novel, constitutively secreted dense granular protein from Toxoplasma gondii
Hye-Jin Ahn, Sehra Kim, Ho-Woo Nam
Biochemical and Biophysical Research Communications.2005; 331(2): 614. CrossRef
USE OF MONOCLONAL ANTIBODIES FOR FLOW CYTOMETRIC DETECTION OF INTRACELLULAR TOXOPLASMA GONDII IN MURINE SPLENIC LYMPHOCYTES
Eun-Hee Shin, Sung-Bum Kim, Ho-Woo Nam, Eun-Taek Han, Jae-Hwan Park, Hye-Jin Ahn, Jong-Yil Chai
Journal of Parasitology.2004; 90(1): 161. CrossRef
Protease activity of 80 kDa protein secreted from the apicomplexan parasite Toxoplasma gondii
Kyoung-Ju Song, Ho-Woo Nam
The Korean Journal of Parasitology.2003; 41(3): 165. CrossRef
Molecular cloning of ribosomal P protein in Toxoplasma gondii and the availability to detect antibody against recombinant protein in toxoplasmosis patients
Hye-Jin Ahn, Sera Kim, Ho-Woo Nam
The Korean Journal of Parasitology.2003; 41(2): 89. CrossRef
Molecular cloning of the 82-kDa heat shock protein (HSP90) of Toxoplasma gondii associated with the entry into and growth in host cells
Hye-Jin Ahn, Sera Kim, Ho-Woo Nam
Biochemical and Biophysical Research Communications.2003; 311(3): 654. CrossRef
Protease Activity and Host Cell Binding of the 42-kDa Rhoptry Protein from Toxoplasma gondii after Secretion
Hye-Jin Ahn, Kyoung-Ju Song, Eui-Sun Son, Jong-Chul Shin, Ho-Woo Nam
Biochemical and Biophysical Research Communications.2001; 287(3): 630. CrossRef
Molecular cloning and characterization of peroxiredoxin from Toxoplasma gondii
Eui-Sun Son, Kyoung-Ju Song, Jong-Chul Shin, Ho-Woo Nam
The Korean Journal of Parasitology.2001; 39(2): 133. CrossRef
Effects of specific monoclonal antibodies to dense granular proteins on the invasion of Toxoplasma gondii in vitro and in vivo
Dong Yeob Cha, In Kwan Song, Gye Sung Lee, Ok-Sun Hwang, Hyung-Jun Noh, Seung-Dong Yeo, Dae-Whan Shin, Young-Ha Lee
The Korean Journal of Parasitology.2001; 39(3): 233. CrossRef