Parasites, Hosts and Diseases

"flow cytometry"

Brief Communication

Effect of Epigallocatechin Gallate on Viability of Kudoa septempunctata: Sang Phil Shin, Hyun Ki Hong, Chang Nam Jin, Hanchang Sohn, Kwang Sik Choi, Jehee Lee; Korean J Parasitol 2020;58(5):593-597.
Published online October 22, 2020; DOI: https://doi.org/10.3347/kjp.2020.58.5.593

Abstract
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Kudoa septempunctata have been reported as a causative agent for acute transient gastrointestinal troubles after eating raw olive flounder (Paralichthys olivaceus). It raised public health concerns and quarantine control in several countries. Quantitative evaluation on viability of K. septempunctata is crucial to develop effective chemotherapeutics against it. A cytometry using fluorescent stains was employed to assess effect of three compounds on viability of K. septempunctata. Epigallocatechin gallate reduced markedly viability of K. septempunctata at 0.5 mM or more, and damaged K. septempunctata spores by producing cracks.

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Original Articles

Non-specific Defensive Factors of the Pacific Oyster Crassostrea gigas against Infection with Marteilioides chungmuensis: A Flow-Cytometric Study: Hee Jung Choi, Jee Youn Hwang, Dong Lim Choi, Min Do Huh, Young Baek Hur, Nam-Sil Lee, Jung Soo Seo, Mun Gyeong Kwon, Hye-Sung Choi, Myoung Ae Park; Korean J Parasitol 2011;49(3):229-234.
Published online September 30, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.3.229

Abstract
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In order to assess changes in the activity of immunecompetency present in Crassostrea gigas infected with Marteilioides chungmuensis (Protozoa), the total hemocyte counts (THC), hemocyte populations, hemocyte viability, and phagocytosis rate were measured in oysters using flow cytometry. THC were increased significantly in oysters infected with M. chungmuensis relative to the healthy appearing oysters (HAO) (P<0.05). Among the total hemocyte composition, granulocyte levels were significantly increased in infected oysters as compared with HAO (P<0.05). In addition, the hyalinocyte was reduced significantly (P<0.05). The hemocyte viability did not differ between infected oysters and HAO. However, the phagocytosis rate was significantly higher in infected oysters relative to HAO (P<0.05). The measurement of alterations in the activity of immunecompetency in oysters, which was conducted via flow cytometry in this study, might be a useful biomarker of the defense system for evaluating the effects of ovarian parasites of C. gigas.

Citations

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Transcriptome-wide identification, functional characterization, and expression analysis of two novel invertebrate-type Toll-like receptors from disk abalone (Haliotis discus discus)
Thanthrige Thiunuwan Priyathilaka, S.D.N.K. Bathige, Seongdo Lee, Bo-Hye Nam, Jehee Lee
Fish & Shellfish Immunology.2019; 84: 802. CrossRef
The dynamics of total organic matter (tom) on sangkuriang catfish (clarias gariepinus) farming at upt ptpbp2kp and the effectiveness of freshwater bivalve (anodonta woodiana) in reducing the total organic matter with varying density
D Arfiati, C D G Putra, A H Tullah, S W A Permanasari, A W Puspitasari
IOP Conference Series: Earth and Environmental Science.2019; 236: 012022. CrossRef
The role of copper and zinc accumulation in defense against bacterial pathogen in the fujian oyster (Crassostrea angulata)
Bo Shi, Tian Wang, Zhen Zeng, Long Zhou, Weiwei You, Caihuan Ke
Fish & Shellfish Immunology.2019; 92: 72. CrossRef
Hybridization improved bacteria resistance in abalone: Evidence from physiological and molecular responses
Shuang Liang, Xuan Luo, Weiwei You, Caihuan Ke
Fish & Shellfish Immunology.2018; 72: 679. CrossRef
Morphofunctional parameters of hemocytes in the assessment of the physiological status of bivalves
A. A. Anisimova
Russian Journal of Marine Biology.2013; 39(6): 381. CrossRef
Immunological responses of the mangrove oysters Crassostrea gasar naturally infected by Perkinsus sp. in the Mamanguape Estuary, Paraíba state (Northeastern, Brazil)
Fernando Ramos Queiroga, Luis Fernando Marques-Santos, Hélène Hégaret, Philippe Soudant, Natanael Dantas Farias, Aline Daiane Schlindwein, Patricia Mirella da Silva
Fish & Shellfish Immunology.2013; 35(2): 319. CrossRef
Relative contribution of reproductive investment, thermal stress and Vibrio infection to summer mortality phenomena in Pacific oysters
Carolin C. Wendling, K. Mathias Wegner
Aquaculture.2013; 412-413: 88. CrossRef
Characteristic and Functional Analysis of Toll-like Receptors (TLRs) in the lophotrocozoan, Crassostrea gigas, Reveals Ancient Origin of TLR-Mediated Innate Immunity
Yang Zhang, Xiaocui He, Feng Yu, Zhiming Xiang, Jun Li, Karen L. Thorpe, Ziniu Yu, Irving Coy Allen
PLoS ONE.2013; 8(10): e76464. CrossRef

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Delayed Human Neutrophil Apoptosis by Trichomonas vaginalis Lysate: Hyun-Ouk Song, Young-Su Lim, Sun-Joo Moon, Myoung-Hee Ahn, Jae-Sook Ryu; Korean J Parasitol 2010;48(1):1-7.
Published online March 17, 2010; DOI: https://doi.org/10.3347/kjp.2010.48.1.1

Abstract
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Neutrophils play an important role in the human immune system for protection against such microorganisms as a protozoan parasite, Trichomonas vaginalis; however, the precise role of neutrophils in the pathogenesis of trichomoniasis is still unknown. Moreover, it is thought that trichomonal lysates and excretory-secretory products (ESP), as well as live T. vaginalis, could possibly interact with neutrophils in local tissues, including areas of inflammation induced by T. vaginalis in humans. The aim of this study was to investigate the influence of T. vaginalis lysate on the fate of neutrophils. We found that T. vaginalis lysate inhibits apoptosis of human neutrophils as revealed by Giemsa stain. Less altered mitochondrial membrane potential (MMP) and surface CD16 receptor expression also supported the idea that neutrophil apoptosis is delayed after T. vaginalis lysate stimulation. In contrast, ESP stimulated-neutrophils were similar in apoptotic features of untreated neutrophils. Maintained caspase-3 and myeloid cell leukemia-1 (Mcl-1) in neutrophils co-cultured with trichomonad lysate suggest that an intrinsic mitochondrial pathway of apoptosis was involved in T. vaginalis lysate-induced delayed neutrophil apoptosis; this phenomenon may contribute to local inflammation in trichomoniasis.

Citations

Citations to this article as recorded by

The role of neutrophils in the pathogenesis of Trichomonas vaginalis infection in pregnant women: A review
Emmanuel Ifeanyi Obeagu, Gideon Ikechukwu Okoroiwu, Nwanganga Ihuoma Ubosi, Getrude Uzoma Obeagu, Ebere Emilia Ayogu, Elham Elamin
Medicine.2025; 104(41): e45063. CrossRef
Trichomonas vaginalis excretory secretory proteins reduce semen quality and male fertility
Zhenchao Zhang, Fakun Li, Yangyang Deng, Yuhua Li, Wanxin Sheng, Xiaowei Tian, Zhenke Yang, Shuai Wang, Lihua Guo, Lixia Hao, Xuefang Mei
Acta Tropica.2023; 238: 106794. CrossRef
Trichomonas vaginalis triggers neutrophil extracellular traps reducing parasite integrity and growth
María G. Ramírez-Ledesma, Yordan J. Romero-Contreras, Mayra C. Rodríguez, Ruth Reyes-Cortes, Patricia Cuéllar-Mata, Eva E. Avila
Parasitology Research.2022; 121(5): 1355. CrossRef
Immune cell arrival kinetics to peritoneum and role during murine-experimental trichomoniasis
F. J. Rangel-Mata, E. E. Ávila-Muro, J. E. Reyes-Martínez, L. M. Olmos-Ortiz, M. E. Brunck, L. A. Arriaga-Pizano, P. Cuéllar-Mata
Parasitology.2021; 148(13): 1624. CrossRef
Trichomonas vaginalis induces apoptosis via ROS and ER stress response through ER–mitochondria crosstalk in SiHa cells
Fei Fei Gao, Juan-Hua Quan, Min A. Lee, Wei Ye, Jae-Min Yuk, Guang-Ho Cha, In-Wook Choi, Young-Ha Lee
Parasites & Vectors.2021;[Epub] CrossRef
Molecular investigation of Trichomoniasis in women in Al-Muthana province /Iraq
H R J Al-abodi, K T M Al-Shaibani, E M Shaker
Journal of Physics: Conference Series.2019; 1234(1): 012078. CrossRef
Amaurocine: Anti- Trichomonas vaginalis protein produced by the basidiomycete Amauroderma camerarium
Mariana Duarte, Adriana Seixas, Maira Peres de Carvalho, Tiana Tasca, Alexandre José Macedo
Experimental Parasitology.2016; 161: 6. CrossRef
Trichomonas vaginalis induces cytopathic effect on human lung alveolar basal carcinoma epithelial cell line A549
Daile Meek C. Salvador-Membreve, Sonia D. Jacinto, Windell L. Rivera
Experimental Parasitology.2014; 147: 33. CrossRef
Trichonomas vaginalis Metalloproteinase Induces Apoptosis of SiHa Cells through Disrupting the Mcl-1/Bim and Bcl-xL/Bim Complexes
Juan-Hua Quan, Byung-Hun Kang, Guang-Ho Cha, Wei Zhou, Young-Bok Koh, Jung-Bo Yang, Heon-Jong Yoo, Min-A Lee, Jae-Sook Ryu, Heung-Tae Noh, Jaeyul Kwon, Young-Ha Lee, A. R. M. Ruhul Amin
PLoS ONE.2014; 9(10): e110659. CrossRef
Altered CD16 expression on vaginal neutrophils from women with vaginitis
Joziani Beghini, Paulo C. Giraldo, Ruth Riboldi, Rose L.G. Amaral, José Eleutério, Steven S. Witkin, Fernando Guimarães
European Journal of Obstetrics & Gynecology and Reproductive Biology.2013; 167(1): 96. CrossRef
Leukotriene B4 receptors BLT1 and BLT2 are involved in interleukin-8 production in human neutrophils induced by Trichomonas vaginalis-derived secretory products
Young Hee Nam, Arim Min, Seong Hoon Kim, Young Ah Lee, Kyeong Ah Kim, Kyoung-Ju Song, Myeong Heon Shin
Inflammation Research.2012; 61(2): 97. CrossRef
NF-κB and CREB Are Involved in IL-8 Production of Human Neutrophils Induced byTrichomonas vaginalis-Derived Secretory Products
Young Hee Nam, Deulle Min, Soon-Jung Park, Kyeong Ah Kim, Young Ah Lee, Myeong Heon Shin
The Korean Journal of Parasitology.2011; 49(3): 291. CrossRef
Trichomoniasis
Jae-Sook Ryu
Hanyang Medical Reviews.2010; 30(3): 213. CrossRef

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T cell phenotype and intracellular IFN-γ production in peritoneal exudate cells and gut intraepithelial lymphocytes during acute Toxoplasma gondii infection in mice: Young-Ha Lee, Dae-Whan Shin; Korean J Parasitol 2002;40(3):119-129.
Published online September 30, 2002; DOI: https://doi.org/10.3347/kjp.2002.40.3.119

Abstract
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Although there are many reports on the splenic (systemic) T cell response after Toxoplasma gondii infection, little information is available regarding the local T cell responses of peritoneal exudate cells (PEC) and gut intraepithelial lymphocytes (IEL) following peroral infection with bradyzoites. Mice were infected with 40 cysts of the 76K strain of T. gondii, and then sacrificed at days 0, 1, 4, 7 and 10 postinfection (PI). The cellular composition and T cell responses of PEC and IEL were analyzed. The total number of PEC and IEL per mouse increased after infection, but the ratio of increase was higher in IEL. Lymphocytes were the major component of both PEC and IEL. The relative percentages of PEC macrophages and neutrophils/eosinophils increased significantly at day 1 and 4 PI, whereas those of IEL did not change significantly. The percentage of PEC NK1.1 and γδ T cells peaked at day 4 PI (p < 0.0001), and CD4 and CD8α T cells increased continuously after infection. The percentages of IEL CD8α and γδ T cells decreased slightly at first, and then increased. CD4 and NK1.1 T cells of IEL did not change significantly after infection. IFN-γ-producing PEC NK1.1 T cells increased significantly from day 1 PI, but the other T cell subsets produced IFN-γ abundantly thereafter. The proportion of IEL IFN-γ-producing CD8α and γδ T cells increased significantly after infection, while IEL NK1.1 T cells had similar IFN-γ production patterns. Taken together, CD4 T cells were the major phenotype and the important IFN-γ-producing T cell subsets in PEC after oral infection with T. gondii, whereas CD8α T cells had these roles in IEL. These results suggest that PEC and IEL comprise different cell differentials and T cell responses, and according to infection route these factors may contribute to the different cellular immune responses.

Citations

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Mucosal and systemic T cell response in mice intragastrically infected with Neospora caninum tachyzoites
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A Requirement for the Vγ1+ Subset of Peripheral γδ T Cells in the Control of the Systemic Growth of Toxoplasma gondii and Infection-Induced Pathology
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