Parasites, Hosts and Diseases

"immunoelectron microscopy"

Original Articles

Ultrastructural localization of 28 kDa glutathione S-transferase in adult Clonorchis sinensis: Sung-Jong Hong, Jae-Ran Yu, Shin-Yong Kang; Korean J Parasitol 2002;40(4):173-176.
Published online December 30, 2002; DOI: https://doi.org/10.3347/kjp.2002.40.4.173

Abstract
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Glutathione S-transferase (28GST) with molecular mass of 28 kDa is an anti-oxidant enzyme abundant in Clonorchis sinensis. In adult C. sinensis, 28GST was localized in tegumental syncytium, cytons, parenchyma, and sperm tails examined by immunoelectron microscopy. C. sinensis 28GST was earlier found to neutralize bio-reactive compounds and to be rich in eggs. Accordingly, it is suggested that 28GST plays important roles in phase II defense system and physiological roles in worm fecundity of C. sinensis.

Citations

Citations to this article as recorded by

Heat-killed Propionibacterium acnes augment the protective effect of 28-kDa glutathione S-transferases antigen against Schistosoma mansoni infection
Ho Yin Pekkle Lam, Ting-Hua Yang, Ting-Ruei Liang, Po-Ching Cheng, Kai-Chih Chang, Shih-Yi Peng
Acta Tropica.2021; 222: 106033. CrossRef
Adult Opisthorchis felineus major protein fractions deduced from transcripts: Comparison with liver flukes Opisthorchis viverrini and Clonorchis sinensis
Mikhail Pomaznoy, Sergey Tatkov, Alexey Katokhin, Dmitry Afonnikov, Vladimir Babenko, Dagmara Furman, Ilya Brusentsov, Pavel Belavin, Alexandr Najakshin, Sergey Guselnikov, Gennady Vasiliev, Anton Sivkov, Egor Prokhortchouk, Konstantin Skryabin, Viatchesl
Experimental Parasitology.2013; 135(2): 297. CrossRef
Identification and biochemical characterization of two novel peroxiredoxins in a liver fluke,Clonorchis sinensis
Y.-A. BAE, S.-H. KIM, E.-G. LEE, W.-M. SOHN, Y. KONG
Parasitology.2011; 138(9): 1143. CrossRef
Progress on the transcriptomics of carcinogenic liver flukes of humans—Unique biological and biotechnological prospects
Neil D. Young, Aaron R. Jex, Cinzia Cantacessi, Bronwyn E. Campbell, Thewarach Laha, Woon-Mok Sohn, Banchob Sripa, Alex Loukas, Paul J. Brindley, Robin B. Gasser
Biotechnology Advances.2010; 28(6): 859. CrossRef
Functional Genes and Proteins of Clonorchis sinensis
Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
The Korean Journal of Parasitology.2009; 47(Suppl): S59. CrossRef
Xenobiotic metabolizing enzymes and metabolism of anthelminthics in helminths
Viktor Cvilink, Jiri Lamka, Lenka Skálová
Drug Metabolism Reviews.2009; 41(1): 8. CrossRef
Opisthorchis viverrini: Gene expression profiling of carcinogenic adult liver fluke worms using 5′ SAGE
Nopporn Chutiwitoonchai, Yan Shen, Huajun Zheng, Hui Xiong, Guoping Zhao, Kanokwan Imtawil, Pewpan M. Intapan, Sopit Wongkham, Chaisiri Wongkham
Experimental Parasitology.2008; 120(4): 306. CrossRef
Clonorchiasis: an update
H.-J. Rim
Journal of Helminthology.2005; 79(3): 269. CrossRef

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Immunoelectron microscopic localization of partially purified antigens in adult Paragonimus iloktsuenensis: Ok-Ran Lee, Pyung-Rim Chung; Korean J Parasitol 2001;39(2):119-132.
Published online June 30, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.2.119

Abstract
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An immunoelectron microscopy employing immunogold labeling method was performed to detect tissue origin of D1 fraction (D1A) among 5 antigenic protein fractions partially purified by DEAE-anion exchange chromatography from water-soluble crude antigen (PIWA) of adult Paragonimus iloktsuenensis. Immune reactions of adult worm tissues with rabbit serum immunoglobulin immunized with crude antigen (PI-Ig) and D1 antigen (D1-Ig), as well as rat serum immunoglobulin infected with P. iloktsuenensis were observed. D1A showed strong antigenicity in the intestinal epithelium of the worms during the early infection period of 2-4 weeks after infection. The vitellaria also showed stronger antigenicity than the other tissue sites in immune reaction of tissues against all immunoglobulins from 4 to 33 weeks after vitelline development. Therefore, it is suggested that D1A was mainly originated from the intestinal epithelial tissues before the development of vitelline gland of the parasites. Immuno-reactivity of two immunoglobulins (PI-Ig, D1-Ig) was significantly different in intestinal epithelial cytoplasmic protrusions (CP) and intestinal epithelial secretory granules (SG). In the experimental group with D1-Ig, gold particles were labeled significantly in CP than in SG when compared to the PI-Ig group. Thus, the major antigenic materials in D1 antigen having a strong antigenicity in the early infection period was considered to be originated from the intestinal epithelial tissue.

Citations

Citations to this article as recorded by

Nuclear ribosomal transcription units in Asian Paragonimus species (Paragonimidae: Platyhelminthes): genetic characteristics, polymorphism, and implications for intersuperfamilial phylogeny
Khue Thi Nguyen, Huong Thi Thanh Doan, Linh Thi Khanh Pham, Do Thi Roan, Takeshi Agatsuma, Pham Ngoc Doanh, Thanh Hoa Le
Parasitology Research.2024;[Epub] CrossRef
Mitogenomic and nuclear ribosomal transcription unit datasets support the synonymy of Paragonimus iloktsuenensis and P. ohirai (Paragonimidae: Platyhelminthes)
Thanh Hoa Le, Khue Thi Nguyen, Linh Thi Khanh Pham, Huong Thi Thanh Doan, Do Thi Roan, Xuyen Thi Kim Le, Takeshi Agatsuma, David Blair
Parasitology Research.2023; 122(7): 1531. CrossRef
Paragonimus westermani infection manifesting as a pulmonary cavity and adrenal gland mass: A case report
Yong Shik Kwon, Hye Won Lee, Hyun Jung Kim
Journal of Infection and Chemotherapy.2019; 25(3): 200. CrossRef
Ion-exchange protocol to obtain antigenic fractions with potential for serodiagnosis of strongyloidiasis
H. T. GONZAGA, C. VILA-VERDE, D. S. NUNES, V. S. RIBEIRO, J. P. CUNHA-JÚNIOR, J. M. COSTA-CRUZ
Parasitology.2013; 140(1): 69. CrossRef