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Case Report

Imported human babesiosis in the Republic of Korea, 2019: two case reports
Hyun Jung Kim, Min Jae Kim, Hyun-Il Shin, Jung-Won Ju, Hee-Il Lee
Parasites Hosts Dis 2023;61(1):72-77.
Published online February 22, 2023
DOI: https://doi.org/10.3347/PHD.22168
Human babesiosis is a tick-borne disease induced by the genus Babesia and has been significantly reported in the Republic of Korea. This report shows the cases of 2 patients with human babesiosis who traveled to the USA in 2019. The 2 patients experienced fever and had travel histories to babesiosis-endemic regions. The diagnoses of both cases were verified by the identification of Babesia-infected red blood cells on blood smears. One patient was found to be infected with Babesia microti using polymerase chain reaction (PCR) for 18S rRNA, which discovered the phylogenetic link to the B. microti strain endemic in the USA. The 2 patients recovered from fever with subsequent hemoparasite clearance. Babesiosis could be diagnosed in anyone with histories of travel to babesiosis-endemic countries and tick bites. Furthermore, Babesia-specific PCR is required for determining geno-and phenotypic characteristics.

Citations

Citations to this article as recorded by  Crossref logo
  • Imported parasitic diseases in the Republic of Korea: status and issues
    Jong-Yil Chai
    Journal of the Korean Medical Association.2025; 68(1): 52.     CrossRef
  • Nationwide investigation of eukaryotic pathogens in ticks from cattle and sheep in Kyrgyzstan using metabarcoding
    Singeun Oh, Nathalie Amvongo-Adjia, Hyun Jung Kim, Jun Ho Choi, Xavier Chavarria, Myung-hee Yi, Arwa Shatta, Bekbolsun Aknazarov, Ju Yeong Kim, Jung-Won Ju, Bekir Oguz
    PLOS One.2025; 20(8): e0327953.     CrossRef
  • Detection of US and Kobe‐type Babesia microti in ticks collected from small mammals of the Republic of Korea
    Tae Yun Kim, Seong Yoon Kim, Jiye Seo, Hee IL Lee, Wook‐Gyo Lee, Hyunwoo Kim
    Entomological Research.2024;[Epub]     CrossRef
  • Human Babesia odocoilei and Bartonella spp. co-infections in the Americas
    Ricardo G. Maggi, Ana Cláudia Calchi, Charlotte O. Moore, Emily Kingston, Edward B. Breitschwerdt
    Parasites & Vectors.2024;[Epub]     CrossRef
  • Development of Multiplex Assays for the Identification of Zoonotic Babesia Species
    Ana Cláudia Calchi, Charlotte O. Moore, Lillianne Bartone, Emily Kingston, Marcos Rogério André, Edward B. Breitschwerdt, Ricardo G. Maggi
    Pathogens.2024; 13(12): 1094.     CrossRef
  • 3,807 View
  • 180 Download
  • 5 Web of Science
  • Crossref

Brief Communication

Diagnosis and Molecular Analysis on Imported Plasmodium ovale curtisi and P. ovale wallikeri Malaria Cases from West and South Africa during 2013-2016
Hyun-Il Shin, Bora Ku, Yu Jung Kim, Tae Yun Kim, Shin-Hyeong Cho, Sang-Eun Lee
Korean J Parasitol 2020;58(1):61-65.
Published online February 29, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.1.61
Majority of the imported malaria cases in Korea is attributed to Plasmodium falciparum and P. vivax infections, whereas P. malariae and P. ovale infections are very rare. Falciparum and ovale malaria are mostly imported from Africa, while most of the vivax malaria cases are imported from Southeast Asia. Here, we report 6 Korean imported ovale malaria cases (4 males and 2 females) who had visited in Africa during 2013-2016. These subjects were diagnosed with P. ovale based on microscopic findings, Plasmodium species-specific nested-PCR, and phylogenetic clade using 18S rRNA gene sequences. We identified 2 P. ovale subtypes, 1 P. ovale curtisi (classic type) and 5 P. ovale wallikeri (variant type). All patients were treated with chloroquine and primaquine, and no relapse or recrudescence was reported for 1 year after treatment. With increase of travelers to the countries where existing Plasmodium species, the risk of Plasmodium infection is also increasing. Molecular monitoring for imported malaria parasites should be rigorously and continuously performed to enable diagnosis and certification of Plasmodium spp.

Citations

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  • Identification of Pharmacophore Groups with Antimalarial Potential in Flavonoids by QSAR-Based Virtual Screening
    Adriana de Oliveira Fernandes, Valéria Vieira Moura Paixão, Yria Jaine Andrade Santos, Eduardo Borba Alves, Ricardo Pereira Rodrigues, Daniela Aparecida Chagas-Paula, Aurélia Santos Faraoni, Rosana Casoti, Marcus Vinicius de Aragão Batista, Marcel Bermude
    Drugs and Drug Candidates.2025; 4(3): 33.     CrossRef
  • Epidemiological Profile and Risk Factors for Malaria in Rural Communities Before the Operationalization of the Singrobo–Ahouaty Dam, Southern Côte d’Ivoire
    Taki Jean Deles Avenié, Kigbafori Dieudonné Silué, Négnorogo Guindo-Coulibaly, Naférima Koné, Sadikou Touré, Kouamé Laurent Valian, Kouassi Séraphin Kouadio, Alloua Marie Joelle Bédia, Boza Fulgence Déabo, Klotcholman Diabagaté, Christian Nsanzabana, Jean
    Tropical Medicine and Infectious Disease.2025; 10(7): 197.     CrossRef
  • Missed Plasmodium ovale Infections Among Symptomatic Persons in Angola, Mozambique, and Ethiopia
    Colleen M Leonard, Jimee Hwang, Ashenafi Assefa, Rose Zulliger, Baltazar Candrinho, Pedro Rafael Dimbu, Abuchahama Saifodine, Mateusz Plucinski, Eric Rogier
    Open Forum Infectious Diseases.2022;[Epub]     CrossRef
  • Elevated plasma α1-antichymotrypsin is a biomarker candidate for malaria patients
    Young Yil Bahk, Sang Bong Lee, Jong Bo Kim, Tong-Soo Kim, Sung-Jong Hong, Dong Min Kim, Sungkeun Lee
    BMB Reports.2022; 55(11): 571.     CrossRef
  • Recent Spatial and Temporal Trends of Malaria in Korea
    Yeong Hoon Kim, Hye-Jin Ahn, Dongjae Kim, Sung-Jong Hong, Tong-Soo Kim, Ho-Woo Nam
    The Korean Journal of Parasitology.2021; 59(6): 585.     CrossRef
  • 7,063 View
  • 152 Download
  • 4 Web of Science
  • Crossref

Original Articles

Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets
Won Gi Yoo, Sun-Min Kim, Eun Jeong Won, Ji-Yun Lee, Fuhong Dai, Ho Choon Woo, Ho-Woo Nam, Tae Im Kim, Jeong-Hee Han, Dongmi Kwak, Yun Sang Cho, Seung-Won Kang, Tong-Soo Kim, Xing-Quan Zhu, Chunren Wang, Heejeong Youn, Sung-Jong Hong
Korean J Parasitol 2018;56(5):437-446.
Published online October 31, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.5.437
To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×103 or 1.0×104 tachyzoites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066-0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative predictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a commercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to supply safe pork on the tables.

Citations

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  • Molecular survey of Toxoplasma gondii B1 gene in pigs from various localities in Korea
    Dongmi Kwak, Min-Goo Seo
    Parasites, Hosts and Diseases.2024; 62(3): 294.     CrossRef
  • Foodborne transmission of Toxoplasma gondii infection in the last decade. An overview
    S. Almeria, J.P. Dubey
    Research in Veterinary Science.2021; 135: 371.     CrossRef
  • Descriptive Comparison of ELISAs for the Detection of Toxoplasma gondii Antibodies in Animals: A Systematic Review
    K. L. D. Tharaka D. Liyanage, Anke Wiethoelter, Jasmin Hufschmid, Abdul Jabbar
    Pathogens.2021; 10(5): 605.     CrossRef
  • All about Toxoplasma gondii infections in pigs: 2009–2020
    Jitender P. Dubey, Camila K. Cerqueira-Cézar, Fernando H.A. Murata, Oliver C.H. Kwok, Dolores Hill, Yurong Yang, Chunlei Su
    Veterinary Parasitology.2020; 288: 109185.     CrossRef
  • WITHDRAWN: All about Toxoplasma gondii infections in pigs: the past decade
    Jitender P. Dubey, Camila K. Cerqueira-Cézar, Fernando H.A. Murata, Oliver C.H. Kwok, Dolores Hill, Yurong R. Yang, Chunlei Su
    Veterinary Parasitology.2020; : 109182.     CrossRef
  • 9,862 View
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  • 4 Web of Science
  • Crossref
Characteristics of Imported Malaria and Species of Plasmodium Involved in Shandong Province, China (2012-2014)
Chao Xu, Qing-Kuan Wei, Jin Li, Ting Xiao, Kun Yin, Chang-Lei Zhao, Yong-Bin Wang, Xiang-Li Kong, Gui-Hua Zhao, Hui Sun, Xin Liu, Bing-Cheng Huang
Korean J Parasitol 2016;54(4):407-414.
Published online August 31, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.4.407
Malaria remains a serious public health problem in Shandong Province, China; therefore, it is important to explore the characteristics of the current malaria prevalence situation in the province. In this study, data of malaria cases reported in Shandong during 2012-2014 were analyzed, and Plasmodium species were confirmed by smear microscopy and nested-PCR. A total of 374 malaria cases were reported, 80.8% of which were reported from 6 prefectures. Of all cases, P. falciparum was dominant (81.3%), followed by P. vivax (11.8%); P. ovale and P. malariae together accounted for 6.4% of cases. Notably, for the first time since 2012, no indigenous case had been reported in Shandong Province, a situation that continued through 2014. Total 95.2% of cases were imported from Africa. The ratio of male/female was 92.5:1, and 96.8% of cases occurred in people 20-54 years of age. Farmers or laborers represented 77.5% of cases. No significant trends of monthly pattern were found in the reported cases. All patients were in good condition after treatment, except for 3 who died. These results indicate that imported malaria has increased significantly since 2012 in Shandong Province, especially for P. falciparum, and there is an emergence of species diversity.

Citations

Citations to this article as recorded by  Crossref logo
  • Toward the Elimination of Malaria in China: A Retrospective Analysis of Malaria-Endemic Characteristics and Prevention Effects in Yantai, Shandong Province, 1951 to 2017
    Xiao Song, Yang Wang, Xiangli Kong, Haifang Wang, Xiaodan Huang, Hongmei Liu, Lijuan Liu, Xiuxia Guo, Chongxing Zhang, Yuqiang Zhao, Jingxuan Kou, Huaiwei Wang, Peng Cheng, Maoqing Gong
    Vector-Borne and Zoonotic Diseases.2020; 20(3): 197.     CrossRef
  • The impact of imported malaria by gold miners in Roraima: characterizing the spatial dynamics of autochthonous and imported malaria in an urban region of Boa Vista
    Jaime Louzada, Nathália Coelho Vargas de Almeida, Joao Luiz Pereira de Araujo, Júlio Silva, Thiago M Carvalho, Ananias A Escalante, Joseli Oliveira-Ferreira
    Memórias do Instituto Oswaldo Cruz.2020;[Epub]     CrossRef
  • Molecular epidemiological surveillance of Africa and Asia imported malaria in Wuhan, Central China: comparison of diagnostic tools during 2011–2018
    Yiting Xie, Kai Wu, Weijia Cheng, Tingting Jiang, Yi Yao, Mingxing Xu, Yan Yang, Huabing Tan, Jian Li
    Malaria Journal.2020;[Epub]     CrossRef
  • Misidentification of Plasmodium ovale as Plasmodium vivax malaria by a microscopic method: a meta-analysis of confirmed P. ovale cases
    Manas Kotepui, Frederick Ramirez Masangkay, Kwuntida Uthaisar Kotepui, Giovanni De Jesus Milanez
    Scientific Reports.2020;[Epub]     CrossRef
  • Monitoring and evaluation of intervals from onset of fever to diagnosis before “1-3-7” approach in malaria elimination: a retrospective study in Shanxi Province, China from 2013 to 2018
    Ting Wang, Shui-Sen Zhou, Jun Feng, Myo Minn Oo, Jing Chen, Chang-Fu Yan, Yi Zhang, Ping Tie
    Malaria Journal.2019;[Epub]     CrossRef
  • Surveillance of Antimalarial Resistance Pfcrt, Pfmdr1, and Pfkelch13 Polymorphisms in African Plasmodium falciparum imported to Shandong Province, China
    Chao Xu, Qingkuan Wei, Kun Yin, Hui Sun, Jin Li, Ting Xiao, Xiangli Kong, Yongbin Wang, Guihua Zhao, Song Zhu, Jingxuan Kou, Ge Yan, Bingcheng Huang
    Scientific Reports.2018;[Epub]     CrossRef
  • Epidemiological analysis of 133 malaria cases in Shanxian county, Shandong Province, China
    Qi-Qi Shi, Peng Cheng, Chong-Xing Zhang, Xiu-Xia Guo, Li-Juan Liu, Hai-Fang Wang, Jing-Xuan Kou, Xiao-Dan Huang, Huai-Wei Wang, Mao-Qing Gong
    Asian Pacific Journal of Tropical Medicine.2017; 10(8): 802.     CrossRef
  • 11,209 View
  • 150 Download
  • 8 Web of Science
  • Crossref
Imported Malaria over Fifteen Years in an Inner City Teaching Hospital of Washington DC
Sri Lakshmi Hyndavi Yeruva, Archana Sinha, Mariam Sarraf-Yazdy, Jhansi Gajjala
Korean J Parasitol 2016;54(3):261-264.
Published online June 30, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.3.261
As endemic malaria is not commonly seen in the United States, most of the cases diagnosed and reported are associated with travel to and from the endemic places of malaria. As the number of imported cases of malaria has been increasing since 1973, it is important to look into these cases to study the morbidity and mortality associated with this disease in the United States. In this study, we would like to share our experience in diagnosing and treating these patients at our institution. We did a retrospective chart review of 37 cases with a documented history of imported malaria from 1998 to 2012. Among them, 16 patients had complicated malaria during that study period, with a mean length of hospital stay of 3.5 days. Most common place of travel was Africa, and chemoprophylaxis was taken by only 11% of patients. Travel history plays a critical role in suspecting the diagnosis and in initiating prompt treatment.

Citations

Citations to this article as recorded by  Crossref logo
  • A Systematic Review and Meta-Analysis of the Proportion Estimates of Disseminated Intravascular Coagulation (DIC) in Malaria
    Thitinat Duangchan, Manas Kotepui, Suriyan Sukati, Yanisa Rattanapan, Kinley Wangdi
    Tropical Medicine and Infectious Disease.2023; 8(6): 289.     CrossRef
  • Prevalence, anti-malarial chemoprophylaxis and causes of deaths for severe imported malaria: A systematic review and meta-analysis
    Aongart Mahittikorn, Wanida Mala, Polrat Wilairatana, Sukhontha Siri, Frederick Ramirez Masangkay, Kwuntida Uthaisar Kotepui, Manas Kotepui
    Travel Medicine and Infectious Disease.2022; 49: 102408.     CrossRef
  • Rapid Discrimination of Malaria- and Dengue-Infected Patients Sera Using Raman Spectroscopy
    Sandip K. Patel, Nishant Rajora, Saurabh Kumar, Aditi Sahu, Sanjay K. Kochar, C. Murali Krishna, Sanjeeva Srivastava
    Analytical Chemistry.2019; 91(11): 7054.     CrossRef
  • The surveillance of four mosquito-borne diseases in international travelers arriving at Guangzhou Baiyun International Airport, China, 2016–2017
    Xue Feng, Wei Sun, Guthrie S. Birkhead, Xuanzhuo Wang, Zhongmin Guo, Jiahai Lu
    Travel Medicine and Infectious Disease.2019; 32: 101513.     CrossRef
  • 8,632 View
  • 111 Download
  • 4 Web of Science
  • Crossref

Case Reports

An Imported Case of Severe Falciparum Malaria with Prolonged Hemolytic Anemia Clinically Mimicking a Coinfection with Babesiosis
Young Ju Na, Jong-Yil Chai, Bong-Kwang Jung, Hyun Jung Lee, Ji Young Song, Ji Hye Je, Ji Hye Seo, Sung Hun Park, Ji Seon Choi, Min Ja Kim
Korean J Parasitol 2014;52(6):667-672.
Published online December 23, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.6.667

While imported falciparum malaria has been increasingly reported in recent years in Korea, clinicians have difficulties in making a clinical diagnosis as well as in having accessibility to effective anti-malarial agents. Here we describe an unusual case of imported falciparum malaria with severe hemolytic anemia lasting over 2 weeks, clinically mimicking a coinfection with babesiosis. A 48-year old Korean man was diagnosed with severe falciparum malaria in France after traveling to the Republic of Benin, West Africa. He received a 1-day course of intravenous artesunate and a 7-day course of Malarone (atovaquone/proguanil) with supportive hemodialysis. Coming back to Korea 5 days after discharge, he was readmitted due to recurrent fever, and further treated with Malarone for 3 days. Both the peripheral blood smears and PCR test were positive for Plasmodium falciparum. However, he had prolonged severe hemolytic anemia (Hb 5.6 g/dl). Therefore, 10 days after the hospitalization, Babesia was considered to be potentially coinfected. A 7-day course of Malarone and azithromycin was empirically started. He became afebrile within 3 days of this babesiosis treatment, and hemolytic anemia profiles began to improve at the completion of the treatment. He has remained stable since his discharge. Unexpectedly, the PCR assays failed to detect DNA of Babesia spp. from blood. In addition, during the retrospective review of the case, the artesunate-induced delayed hemolytic anemia was considered as an alternative cause of the unexplained hemolytic anemia.

Citations

Citations to this article as recorded by  Crossref logo
  • Imported parasitic diseases in the Republic of Korea: status and issues
    Jong-Yil Chai
    Journal of the Korean Medical Association.2025; 68(1): 52.     CrossRef
  • Current Status of Trypanosoma grosi and Babesia microti in Small Mammals in the Republic of Korea
    Hyun Jung Kim, BoGyeong Han, Hee-Il Lee, Jung-Won Ju, Hyun-Il Shin
    Animals.2024; 14(7): 989.     CrossRef
  • Imported human babesiosis in the Republic of Korea, 2019: two case reports
    Hyun Jung Kim, Min Jae Kim, Hyun-Il Shin, Jung-Won Ju, Hee-Il Lee
    Parasites, Hosts and Diseases.2023; 61(1): 72.     CrossRef
  • Babesia microti alleviates disease manifestations caused by Plasmodium berghei ANKA in murine co-infection model of complicated malaria
    Iqra Zafar, Tomoyo Taniguchi, Hanadi B. Baghdadi, Daisuke Kondoh, Mohamed Abdo Rizk, Eloiza May Galon, Shengwei Ji, Shimaa Abd El-Salam El-Sayed, Thom Do, Hang Li, Moaz M. Amer, Ma Zhuowei, Ma Yihong, Jinlin Zhou, Noboru Inoue, Xuenan Xuan
    Frontiers in Cellular and Infection Microbiology.2023;[Epub]     CrossRef
  • A conserved protein of Babesia microti elicits partial protection against Babesia and Plasmodium infection
    Yao Wang, Qianqian Zhang, Wanruo Zhang, Junhu Chen, Jianfeng Dai, Xia Zhou
    Parasites & Vectors.2023;[Epub]     CrossRef
  • Babesia microti Confers Macrophage-Based Cross-Protective Immunity Against Murine Malaria
    Artemis Efstratiou, Eloiza May S. Galon, Guanbo Wang, Kousuke Umeda, Daisuke Kondoh, Mohamad Alaa Terkawi, Aiko Kume, Mingming Liu, Aaron Edmond Ringo, Huanping Guo, Yang Gao, Seung-Hun Lee, Jixu Li, Paul Franck Adjou Moumouni, Yoshifumi Nishikawa, Hirosh
    Frontiers in Cellular and Infection Microbiology.2020;[Epub]     CrossRef
  • Two Imported Cases of Babesiosis with Complication or Co-Infection with Lyme Disease in Republic of Korea
    Hea Yoon Kwon, Jae Hyoung Im, Yun-Kyu Park, Areum Durey, Jin-Soo Lee, Ji Hyeon Baek
    The Korean Journal of Parasitology.2018; 56(6): 609.     CrossRef
  • Delayed Diagnosis of Falciparum Malaria with Acute Kidney Injury
    Iee Ho Choi, Pyoung Han Hwang, Sam Im Choi, Dae-Yeol Lee, Min Sun Kim
    Journal of Korean Medical Science.2016; 31(9): 1499.     CrossRef
  • Malaria endemicity and co-infection with tissue-dwelling parasites in Sub-Saharan Africa: a review
    Nyamongo W. Onkoba, Moses J. Chimbari, Samson Mukaratirwa
    Infectious Diseases of Poverty.2015;[Epub]     CrossRef
  • Artesunate

    Reactions Weekly.2015; 1574(1): 36.     CrossRef
  • 10,646 View
  • 107 Download
  • 9 Web of Science
  • Crossref
A Case of Plasmodium ovale wallikeri Infection in a Chinese Worker Returning from West Africa
Yuchun Li, Guangze Wang, Dingwei Sun, Feng Meng, Shigan Lin, Ximin Hu, Shanqing Wang
Korean J Parasitol 2013;51(5):557-562.
Published online October 31, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.5.557

In contrast to the gradual reduction in the number of locally transmitted malaria cases in China, the number of imported malaria cases has been increasing since 2008. Here, we report a case of a 39-year-old Chinese man who acquired Plasmodium ovale wallikeri infection while staying in Ghana, West Africa for 6 months in 2012. Microscopic examinations of Giemsa-stained thin and thick blood smears indicated Plasmodium vivax infection. However, the results of rapid diagnostic tests, which were conducted 3 times, were not in agreement with P. vivax. To further check the diagnosis, standard PCR analysis of the small-subunit rRNA gene was conducted, based on which a phylogeny tree was constructed. The results of gene sequencing indicated that this malaria is a variant of P. ovale (P. ovale wallikeri). The infection in this patient was not a new infection, but a relapse of the infection from the one that he had contracted in West Africa.

Citations

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  • Geographical origin of Plasmodium vivax in the Hainan Island, China: insights from mitochondrial genome
    Yuchun Li, Xiaomin Huang, Ling Qing, Wen Zeng, Xiangjie Zeng, Feng Meng, GuangZe Wang, Yan Chen
    Malaria Journal.2023;[Epub]     CrossRef
  • An innovative three-layer strategy in response to a quartan malaria outbreak among forest goers in Hainan Island, China: a retrospective study
    Yuchun Li, Yingjuan Huang, Renqiang Chen, Weizhen Huang, Huanzhi Xu, Rongshen Ye, Shaoling Huang, Ji Zhen, Xiaodan Wen, Guoyi Wang, Yong Liu, Haishan Li, Zaichun Zheng, Jian Wang, Guoshen Wang, Chong Chen, Wen Zeng, Feng Meng, Xiaoming Huang, Guangze Wang
    Infectious Diseases of Poverty.2022;[Epub]     CrossRef
  • High frequency of the Duffy-negative genotype and absence of Plasmodium vivax infections in Ghana
    Charles A. Brown, Prince J. Pappoe-Ashong, Nancy Duah, Anita Ghansah, Harry Asmah, Edwin Afari, Kwadwo A. Koram
    Malaria Journal.2021;[Epub]     CrossRef
  • A comparison of two PCR protocols for the differentiation of Plasmodium ovale species and implications for clinical management in travellers returning to Germany: a 10-year cross-sectional study
    Hagen Frickmann, Christine Wegner, Stefanie Ruben, Ulrike Loderstädt, Egbert Tannich
    Malaria Journal.2019;[Epub]     CrossRef
  • Indel-informed Bayesian analysis suggests cryptic population structure between Plasmodium knowlesi of humans and long-tailed macaques (Macaca fascicularis) in Malaysian Borneo
    JustinJ.S. Wilcox, Abigail Kerschner, Hope Hollocher
    Infection, Genetics and Evolution.2019; 75: 103994.     CrossRef
  • Detection of malaria with light microscopy and Nested polymerase chain reaction (Nested PCR) methods in peripheral blood expansions and investigation of the genetic diversity of Plasmodium species by 18S rRNA gene in Southeast of Iran
    Ahmad Taghdiri, Pooya Ghasemi Nejad Almani, Iraj Sharifi, Mohammad Ali Mohammadi, Samira Salari
    Microbial Pathogenesis.2019; 137: 103782.     CrossRef
  • WITHDRAWN: Indel-informed bayesian analysis suggests cryptic divisions between Plasmodium knowlesi of humans and long-tailed macaques (Macaca fascicularis) in Malaysian Borneo
    Justin J.S. Wilcox, Abigail Kerschner, Hope Hollocher
    Infection, Genetics and Evolution.2018;[Epub]     CrossRef
  • Diagnosis of an imported Plasmodium ovale wallikeri infection in Malaysia
    Jonathan Wee Kent Liew, Rohela Mahmud, Lian Huat Tan, Yee Ling Lau
    Malaria Journal.2016;[Epub]     CrossRef
  • Clinical implications of a gradual dormancy concept in malaria
    Joachim Richter, Gabriele Franken, Martha C. Holtfreter, Susanne Walter, Alfons Labisch, Heinz Mehlhorn
    Parasitology Research.2016; 115(6): 2139.     CrossRef
  • Genetic diversity of Plasmodium vivax population before elimination of malaria in Hainan Province, China
    Yu-Chun Li, Guang-Ze Wang, Feng Meng, Wen Zeng, Chang-hua He, Xi-Min Hu, Shan-Qing Wang
    Malaria Journal.2015;[Epub]     CrossRef
  • Molecular characterization of misidentified Plasmodium ovale imported cases in Singapore
    Jean-Marc Chavatte, Sarah Bee Hui Tan, Georges Snounou, Raymond Tzer Pin Valentine Lin
    Malaria Journal.2015;[Epub]     CrossRef
  • Characterization of Plasmodium ovale curtisi and P. ovale wallikeri in Western Kenya Utilizing a Novel Species-specific Real-time PCR Assay
    Robin H. Miller, Clifford O. Obuya, Elizabeth W. Wanja, Bernhards Ogutu, John Waitumbi, Shirley Luckhart, V. Ann Stewart, Alain Debrabant
    PLOS Neglected Tropical Diseases.2015; 9(1): e0003469.     CrossRef
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A Case of Plasmodium ovale Malaria Imported from West Africa
Yunjung Kang, Jinyoung Yang
Korean J Parasitol 2013;51(2):213-218.
Published online April 25, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.2.213

Malaria is a parasitic infection caused by Plasmodium species. Most of the imported malaria in Korea are due to Plasmodium vivax and Plasmodium falciparum, and Plasmodium ovale infections are very rare. Here, we report a case of a 24-year-old American woman who acquired P. ovale while staying in Ghana, West Africa for 5 months in 2010. The patient was diagnosed with P. ovale malaria based on a Wright-Giemsa stained peripheral blood smear, Plasmodium genus-specific real-time PCR, Plasmodium species-specific nested PCR, and sequencing targeting 18S rRNA gene. The strain identified had a very long incubation period of 19-24 months. Blood donors who have malaria with a very long incubation period could be a potential danger for propagating malaria. Therefore, we should identify imported P. ovale infections not only by morphological findings but also by molecular methods for preventing propagation and appropriate treatment.

Citations

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  • A case of an asymptomatic Plasmodium falciparum infection followed by a symptomatic Plasmodium ovale infection in a soldier deployed to South Sudan
    Choon Mee Kim, Jun-Won Seo, Da Young Kim, Na Ra Yun, Beomgi Lee, You Mi Lee, Munawir Muhammad, Dong-Min Kim
    Malaria Journal.2025;[Epub]     CrossRef
  • Plasmodium ovale: Exploring an Atypical Presentation
    Priscila Lopez, Samah Suleiman, Mario Valdez Imbert, Mark N Sayegh, Tjark Schliep
    Cureus.2024;[Epub]     CrossRef
  • Diagnosis and Molecular Analysis on Imported Plasmodium ovale curtisi and P. ovale wallikeri Malaria Cases from West and South Africa during 2013-2016
    Hyun-Il Shin, Bora Ku, Yu Jung Kim, Tae Yun Kim, Shin-Hyeong Cho, Sang-Eun Lee
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    Joachim Richter, Gabriele Franken, Martha C. Holtfreter, Susanne Walter, Alfons Labisch, Heinz Mehlhorn
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    Yun-Jung Kang, Moon-Jung Shim, Jung-Yeon Kim, So-Young Ji, Won-Ja Lee, Jinyoung Yang
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    Miles B. Markus
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  • A molecular survey of acute febrile illnesses reveals Plasmodium vivax infections in Kedougou, southeastern Senegal
    Makhtar Niang, Laty Gaye Thiam, Abdourahmane Sow, Cheikh Loucoubar, Ndeye Sakha Bob, Fode Diop, Babacar Diouf, Oumy Niass, Annick Mansourou, Marie Louise Varela, Ronald Perraut, Amadou A Sall, Aissatou Toure-Balde
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  • New type of SSUrDNA sequence was detected from both Plasmodium ovale curtisi and Plasmodium ovale wallikeri samples
    Mei Li, Zhigui Xia, He Yan
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    Heather Senn, Nadia Alattas, Andrea K Boggild, Shaun K Morris
    Malaria Journal.2014;[Epub]     CrossRef
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    Yuchun Li, Guangze Wang, Dingwei Sun, Feng Meng, Shigan Lin, Ximin Hu, Shanqing Wang
    The Korean Journal of Parasitology.2013; 51(5): 557.     CrossRef
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Brief Communication

Imported Malaria in Korea: a 13-Year Experience in a Single Center
Hae Suk Cheong, Ki-Tae Kwon, Ji-Young Rhee, Seong Yeol Ryu, Dong Sik Jung, Sang Taek Heo, Sang Yop Shin, Doo Ryun Chung, Kyong Ran Peck, Jae-Hoon Song
Korean J Parasitol 2009;47(3):299-302.
Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.299

The incidence of imported malaria has been increasing in Korea. We reviewed data retrospectively to evaluate the epidemiology, clinical features, and outcomes of imported malaria from 1995 to 2007 in a university hospital. All patients diagnosed with imported malaria were included. Imported malaria was defined as a positive smear for malaria that was acquired in a foreign country. A total of 49 patients (mean age, 35.7 year; M : F = 38 : 11) were enrolled. The predominant malarial species was Plasmodium falciparum (73.5%), and the most frequent area of acquisition was Africa (55.1%), followed by Southeast Asia (22.4%) and South Asia (18.4%). Fourteen-patients (30.6%) suffered from severe malaria caused by P. falciparum and 1 patient (2.0%) died of multiorgan failure. Most of the patients were treated with mefloquine (79.2%) or quinine (10.2%); other antimalarial agents had to be given in 13.2% treated with mefloquine and 44.4% with quinine due to adverse drug events (ADEs). P. falciparum was the most common cause of imported malaria, with the majority of cases acquired from Africa, and a significant number of patients had severe malaria. Alternative antimalarial agents with lower rates of ADEs might be considered for effective treatment instead of mefloquine and quinine.

Citations

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  • Imported parasitic diseases in the Republic of Korea: status and issues
    Jong-Yil Chai
    Journal of the Korean Medical Association.2025; 68(1): 52.     CrossRef
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    Mile Bosilkovski, Bachir Khezzani, Kostadin Poposki, Vesna Semenakova-Cvetkovska, Ivan Vidinic, Arlinda Osmani Lloga, Dejan Jakimovski, Marija Dimzova
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  • Prevalence, anti-malarial chemoprophylaxis and causes of deaths for severe imported malaria: A systematic review and meta-analysis
    Aongart Mahittikorn, Wanida Mala, Polrat Wilairatana, Sukhontha Siri, Frederick Ramirez Masangkay, Kwuntida Uthaisar Kotepui, Manas Kotepui
    Travel Medicine and Infectious Disease.2022; 49: 102408.     CrossRef
  • Malaria Cases in a Tertiary Hospital in Kuala Lumpur, Malaysia: A 16-Year (2005–2020) Retrospective Review
    Nor Diyana Dian, Ahmad Firdaus Mohd Salleh, Mohd Amirul Fitri A Rahim, Mohd Bakhtiar Munajat, Siti Nor Azreen Abd Manap, Nuraffini Ghazali, Noor Wanie Hassan, Zulkarnain Md Idris
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  • Genetic Diversity and Distribution ofBlastocystisSubtype 3 in Human Populations, with Special Reference to a Rural Population in Central Mexico
    Liliana Rojas-Velázquez, Patricia Morán, Angélica Serrano-Vázquez, Leonardo D. Fernández, Horacio Pérez-Juárez, Augusto C. Poot-Hernández, Tobías Portillo, Enrique González, Eric Hernández, Oswaldo Partida-Rodríguez, Miriam E. Nieves-Ramírez, Ulises Magañ
    BioMed Research International.2018; 2018: 1.     CrossRef
  • An Imported Case of Severe Falciparum Malaria with Prolonged Hemolytic Anemia Clinically Mimicking a Coinfection with Babesiosis
    Young Ju Na, Jong-Yil Chai, Bong-Kwang Jung, Hyun Jung Lee, Ji Young Song, Ji Hye Je, Ji Hye Seo, Sung Hun Park, Ji Seon Choi, Min Ja Kim
    The Korean Journal of Parasitology.2014; 52(6): 667.     CrossRef
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    N Ben Alaya-Bouafif, MK Chahed, H El Bez, H Bellali, L Ayari, N Achour
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    Myoung-Hee Ahn
    Infection and Chemotherapy.2010; 42(5): 271.     CrossRef
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Original Articles

Imported Malaria in United Arab Emirates: Evaluation of a New DNA Extraction Technique Using Nested PCR
Doaa M. Sultan, Marwa M. Khalil, Ahmed S. Abdouh, Wafaa F. Doleh, Abdul Aziz M. Al Muthanna
Korean J Parasitol 2009;47(3):227-233.
Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.227

Local malaria transmission in the United Arab Emirates (UAE) came to an end in 1997. Nevertheless, UAE has been subjected to substantial importation of malaria cases from abroad, concerning both UAE nationals and immigrants from malarious countries with a total number of 2,119 cases in 2007. To evaluate a new DNA extraction technique using nested PCR, blood samples were collected from 132 individuals who presented to Infectious Diseases Department in Rashid Hospital, Dubai, and Central Department of Malaria Control with fever and persistent headache. Giemsa-stained blood films and ELISA test for malaria antibodies were carried out for detection of Plasmodium infection. Plasmodium infections were identified with the genus-specific primer set and species differentiation using nested PCR. A rapid procedure for diagnosis of malaria infections directly from dried blood spots using for the first time DNA extract from FTA Elute cards was evaluated in contrast to extraction techniques using FTA classic cards and rapid boiling technique. Our new simple technique for DNA extraction using FTA Elute cards was very sensitive giving a sensitivity of 100% compared to 94% using FTA classic cards and 62% in the rapid boiling technique. No complex preparation of blood samples was required prior to the amplification. The production cost of DNA isolation in our PCR assay was much less in comparable to that of other DNA extraction protocols. The nested PCR detected plasmodial infection and could differentiate P. falciparum from P. vivax, and also detected the mixed infection.

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  • Nucleic acid purification from dried blood spot on FTA Elute Card provides template for polymerase chain reaction for highly sensitive Plasmodium detection
    Muneaki Hashimoto, Mika Bando, Jun-ichi Kido, Kazumichi Yokota, Toshihiro Mita, Kazuaki Kajimoto, Masatoshi Kataoka
    Parasitology International.2019; 73: 101941.     CrossRef
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    Mohammed H. Abdelraheem, Devendra Bansal, Mohammed A. Idris, Moawia M. Mukhtar, Muzamil M. Abdel Hamid, Zainb S. Imam, Sisay Getachew, Rakesh Sehgal, Hargobinder Kaur, Amal H. Gadalla, Salam Al-Hamidhi, Zainab Al-Hashami, Ali Al-Jabri, Ali A. Sultan, Hamz
    Scientific Reports.2018;[Epub]     CrossRef
  • A simple, efficient and inexpensive method for malaria parasites’ DNA catching from fixed Giemsa-stained blood slides
    Abbas Ali Eskandarian, Sara Moradi, Saeed Abedi
    Journal of Parasitic Diseases.2016; 40(3): 833.     CrossRef
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    Salama Al-Hamidhi, Mohammed AK Mahdy, Zainab Al-Hashami, Hissa Al-Farsi, Abdulsalam M Al-mekhlafi, Mohamed A Idris, Albano Beja-Pereira, Hamza A Babiker
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    Sepideh Seghatoleslami, Lars Ohlsson, Kristina Hamberg, Peter Carlsson, Dan Ericson, Lennart Ljunggren
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    Tara L. Klassen, Eva-Lotta von Rüden, Janice Drabek, Jeffrey L. Noebels, Alica M. Goldman
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    Hissa M. Al-Farsi, Zainab S. Al-Hashami, Saad M. Bin Dajem, Adel Ali H. Al-Sheikh, Ahmed Al-Qahtani, Albano Beja-Pereira, Mohamed A. Idris, Hamza A. Babiker
    Infection, Genetics and Evolution.2012; 12(6): 1253.     CrossRef
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Travelers' malaria among foreigners at the Hospital for Tropical Diseases, Bangkok, Thailand: a 6-year review (2000-2005)
Watcharapong Piyaphanee, Srivicha Krudsood, Udomsak Silachamroon, Karnchana Pornpininworakij, Phatcharee Danwiwatdecha, Supat Chamnachanan, Polrat Wilairatana, Sornchai Looareesuwan
Korean J Parasitol 2006;44(3):229-232.
Published online September 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.3.229

We retrospectively examined the charts of travelers admitted to the Hospital for Tropical Diseases, Bangkok, Thailand, with malaria during the years 2000-2005. Twenty-one cases of malaria were identified, of which 12 (57%) were Plasmodium vivax infections and 9 (43%) were P. falciparum infections. There was one mixed case with vivax and falciparum infection. Only 1 P. falciparum case had complications. All cases were successfully treated with standard antimalarial drugs. Only 3 of the 21 cases were thought to be acquired in Thailand, the rest were regarded to be imported.

Citations

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  • Epidemiologic trends and clinical outcomes of imported malaria in a tertiary care hospital, Bangkok, Thailand: A retrospective analysis (2013–2022)
    Panita Looareesuwan, Rachata Charoenwisedsil, Punyisa Asawapaithulsert, Phimphan Pisutsan, Viravarn Luvira, Watcharapong Piyaphanee, Wasin Matsee
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    Annelies Wilder-Smith
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    Saranath Lawpoolsri, Irwin F Chavez, Surapon Yimsamran, Supalap Puangsa-art, Nipon Thanyavanich, Wanchai Maneeboonyang, Wuthichai Chaimungkun, Pratap Singhasivanon, James H Maguire, Laura L Hungerford
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    Panita Gosi, Srisin Khusmith, Thareerat Khalambaheti, David E Lanar, Kurt E Schaecher, Mark M Fukuda, Scott R Miller
    Malaria Journal.2008;[Epub]     CrossRef
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  • 86 Download
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Case Report
Three clinical cases of cutaneous larva migrans
Tae-Heung Kim, Byeung-Song Lee, Woon-Mok Sohn
Korean J Parasitol 2006;44(2):145-149.
Published online June 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.2.145

Three cases of cutaneous larva migrans (CLM) were diagnosed in a returnee from a trip to Thailand and in 2 domestic farmers during July and September, 2003. The linear and serpiginous skin lesions on the lower extremities were presented in all 3 cases. Routine laboratory findings were normal. In the imported case, a 650 × 30 ?m sized filariform nematode larva, presumably a species of hookworm, was detected in the lesion. All cases were treated with 400 mg albendazole once daily for 3-5 days, and their skin lesions gradually improved. In the present study, a causative agent of CLM was isolated for the first time in the Republic of Korea. Moreover, we speculate that CLM is prevalent in farmers who are in frequent contact with soil in the Republic of Korea.

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    Jong-Yil Chai
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    Vijayasankar Palaniappan, Hima Gopinath, Kaliaperumal Karthikeyan
    Clinical and Experimental Dermatology.2025;[Epub]     CrossRef
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    Angela Stufano, Caterina Foti, Piero Lovreglio, Paolo Romita, Aurora De Marco, Riccardo Paolo Lia, Domenico Otranto, Roberta Iatta, Adly M.M. Abd-Alla
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    Digestive Diseases and Sciences.2021; 66(1): 105.     CrossRef
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    Selvam Kannathasan, Arumugam Murugananthan, Nadarajah Rajeshkannan, Nilanthi Renuka de Silva, Ana Paula Arez
    PLoS ONE.2013; 8(4): e61816.     CrossRef
  • Cutaneous Larva Migrans among Devotees of the Nallur Temple in Jaffna, Sri Lanka
    Selvam Kannathasan, Arumugam Murugananthan, Nadarajah Rajeshkannan, Nilanthy Renuka de Silva, Jose Antonio Stoute
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    Kyoung-Hwan Joo
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    Myoung-Hee Ahn
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    Israel Alfonso Trujillo, María Antonia Rodríguez García, Sonia Collazo Caballero, Jonathan Arguello Moya
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    Myoung-Hee Ahn
    Journal of the Korean Medical Association.2007; 50(11): 993.     CrossRef
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    Jong-Yil Chai
    Journal of the Korean Medical Association.2007; 50(11): 946.     CrossRef
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